Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 ...read more
Jurkat human acute T cell leukemia cell line treated with 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more
Smad4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) at 15 µg/mL for 3 hours at room ...read more
E. coli-derived recombinant human Smad4 Pro139-Asp332 Accession # Q13485
Detects human Smad4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) Smad1 and less than 5% cross-reactivity with rhSmad9, rhSmad5, and rhSmad7 is observed.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Smad4 Antibody [Unconjugated]
DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
MAD homolog 4
MADH4mothers against decapentaplegic homolog 4
mothers against decapentaplegic homolog 4
mothers against decapentaplegic, Drosophila, homolog of, 4
SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
SMAD, mothers against DPP homolog 4
Smads are a family of intracellular proteins that transmit transforming
growth factor beta (TGF-beta ) superfamily signals from the cell surface to
the nucleus. Upon signal-induced phosphorylation, Smad subunits
associate with the common-mediator subunit, Smad4. This heteromeric
complex then translocates into the nucleus to exert transcriptional
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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