Recombinant Human BMP-6 Protein

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1 μg/lane of Recombinant Human BMP-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 18 kDa and 32 kDa, respectively.
Recombinant Human BMP-6 (Catalog #507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.02‑0.15 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-6 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.02‑0.15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-6 protein
Gln382-His513
Accession #
N-terminal Sequence
Gln382
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP6
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18 kDa, reducing conditions
Publications
Read Publications using
507-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-6 Protein

  • BMP6
  • BMP-6
  • bone morphogenetic protein 6
  • vegetal related growth factor (TGFB-related)
  • vegetal-related (TGFB related) cytokine
  • VG-1-R
  • VG-1-related protein
  • Vg1-related sequence
  • VGR
  • VGR1
  • Vgr-1

Background

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). Human BMP-6 is synthesized as a 513 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, although it can also form heterodimers with mature BMP-2 (2, 3). Mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (4, 5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP coreceptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).
  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

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Publications for BMP-6 (507-BP)(49)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Ovine.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(43)
In Vivo
(1)
Surface Plasmon Resonance
(3)
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Human
(36)
Mouse
(10)
Bovine
(1)
Ovine
(1)
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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 507-BP Applications Species
A Moghieb, L Tesfay, S Nie, M Gritsenko, TL Fillmore, JM Jacobs, RD Smith, FM Torti, SV Torti, T Shi, C Ansong A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification Sci Rep, 2019;9(1):7264. 2019 [PMID: 31086210] (Bioassay, Human) Bioassay Human
S Nürnberger, C Lindner, J Maier, K Strohmeier, C Wurzer, P Slezak, S Suessner, W Holnthoner, H Redl, S Wolbank, E Priglinger Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction Eur Cell Mater, 2019;37(0):113-133. 2019 [PMID: 30793275] (Bioassay, Human) Bioassay Human
J Lee, SJ Kwon, JH Kim, H Jang, NK Lee, JW Hwang, JH Kim, JW Chang, DL Na Cerebrospinal fluid from Alzheimer&#039;s disease patients as an optimal formulation for therapeutic application of mesenchymal stem cells in Alzheimer&#039;s disease Sci Rep, 2019;9(1):564. 2019 [PMID: 30679696] (Bioassay, Human) Bioassay Human
Y Ogata, Y Mabuchi, K Shinoda, Y Horiike, M Mizuno, K Otabe, EG Suto, N Suzuki, I Sekiya, C Akazawa Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage Regen Ther, 2018;8(0):20-28. 2018 [PMID: 30271862] (Bioassay, Human) Bioassay Human
T Peeters, S Monteagudo, P Tylzanowsk, FP Luyten, R Lories, F Cailotto SMOC2 inhibits calcification of osteoprogenitor and endothelial cells PLoS ONE, 2018;13(6):e0198104. 2018 [PMID: 29897942] (Bioassay, Human) Bioassay Human
SR Pasricha, PJ Lim, TL Duarte, C Casu, D Oosterhuis, K Mleczko-Sa, M Suciu, AR Da Silva, K Al-Hourani, J Arezes, K McHugh, S Gooding, JN Frost, K Wray, A Santos, G Porto, E Repapi, N Gray, SJ Draper, N Ashley, E Soilleux, P Olinga, MU Muckenthal, JR Hughes, S Rivella, TA Milne, AE Armitage, H Drakesmith Hepcidin is regulated by promoter-associated histone acetylation and HDAC3 Nat Commun, 2017;8(1):403. 2017 [PMID: 28864822] (Bioassay, Human) Bioassay Human
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Bioassay, Mouse) Bioassay Mouse
CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2 J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28646109] (Bioassay, Human) Bioassay Human
LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5 PLoS ONE, 2017;12(5):e0177188. 2017 [PMID: 28489883] (Bioassay, Human) Bioassay Human
K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke BMP-9 interferes with liver regeneration and promotes liver fibrosis Gut, 2017;0(0):. 2017 [PMID: 28336518] (Mouse) Mouse
Show All 49 Publications.

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Bioinformatics

Gene Symbol BMP6
Uniprot