Recombinant Human BMP-6 Protein


Recombinant Human BMP-6 (Catalog #507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.02‑0.15 μg/mL.
1 μg/lane of Recombinant Human BMP-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 18 kDa and 32 kDa, respectively.

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Recombinant Human BMP-6 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.02‑0.15 µg/mL.
Mouse myeloma cell line, NS0-derived human BMP-6 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
15 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
18 kDa, reducing conditions
Read Publications using
507-BP in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-6 Protein

  • BMP6
  • BMP-6
  • bone morphogenetic protein 6
  • vegetal related growth factor (TGFB-related)
  • vegetal-related (TGFB related) cytokine
  • VG-1-R
  • VG-1-related protein
  • Vg1-related sequence
  • VGR
  • VGR1
  • Vgr-1


Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). Human BMP-6 is synthesized as a 513 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, although it can also form heterodimers with mature BMP-2 (2, 3). Mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (4, 5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP coreceptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).
  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

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Publications for BMP-6 (507-BP)(70)

We have publications tested in 6 confirmed species: Human, Mouse, Bovine, Goat, Ovine, Porcine.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, In Vivo, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using 507-BP Applications Species
M Bigorra Mi, E Charlebois, S Tsyplenkov, C Fillebeen, K Pantopoulo Cardiac Hamp mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron International Journal of Molecular Sciences, 2023-03-08;24(6):. 2023-03-08 [PMID: 36982241] (Bioassay, Human) Bioassay Human
N Ozeki, M Mizuno, S Yanada, T Okada, R Kubota, Y Kushida, H Furuoka, K Endo, H Katano, K Nakamura, S Ohya, H Koga, I Sekiya Autologous Synovial Mesenchymal Stem Cell Transplantation Suppresses Inflammation Caused by Synovial Harvesting and Promotes Healing in a Micro Minipig Repaired Meniscus Model Transplantation Proceedings, 2023-03-10;0(0):. 2023-03-10 [PMID: 36906438] (Bioassay, Porcine) Bioassay Porcine
L Wang, Y Wang, B Li, Y Zhang, S Song, W Ding, D Xu, Z Zhao BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells Theriogenology, 2022-11-30;197(0):167-176. 2022-11-30 [PMID: 36525856] (Bioassay, Goat) Bioassay Goat
Y Soejima, N Iwata, N Nakayama, S Hirata, Y Nakano, K Yamamoto, A Suyama, K Oguni, T Nada, S Fujisawa, F Otsuka Mutual Effects of Orexin and Bone Morphogenetic Proteins on Gonadotropin Expression by Mouse Gonadotrope Cells International Journal of Molecular Sciences, 2022-08-29;23(17):. 2022-08-29 [PMID: 36077179] (Bioassay, Mouse) Bioassay Mouse
ALM Caldwell, L Sancho, J Deng, A Bosworth, A Miglietta, JK Diedrich, MN Shokhirev, NJ Allen Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders Nature Neuroscience, 2022-08-30;25(9):1163-1178. 2022-08-30 [PMID: 36042312] (Bioassay, Mouse) Bioassay Mouse
Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis Cell Reports, 2022-05-10;39(6):110805. 2022-05-10 [PMID: 35545056] (Bioassay, Human) Bioassay Human
Y Soejima, N Iwata, Y Nakano, K Yamamoto, A Suyama, T Nada, F Otsuka Biphasic Roles of Clock Genes and Bone Morphogenetic Proteins in Gonadotropin Expression by Mouse Gonadotrope Cells International Journal of Molecular Sciences, 2021-10-17;22(20):. 2021-10-17 [PMID: 34681844] (Cell Culture, Human) Cell Culture Human
P Jiang, Y Zhang, B Ru, Y Yang, T Vu, R Paul, A Mirza, G Altan-Bonn, L Liu, E Ruppin, L Wakefield, KW Wucherpfen Systematic investigation of cytokine signaling activity at the tissue and single-cell levels Nature methods, 2021-09-30;18(10):1181-1191. 2021-09-30 [PMID: 34594031] (Bioassay, Human) Bioassay Human
R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily Scientific Reports, 2021-09-15;11(1):18341. 2021-09-15 [PMID: 34526551] (Bioassay, Human) Bioassay Human
E Charlebois, K Pantopoulo Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes PLoS ONE, 2021-06-23;16(6):e0253475. 2021-06-23 [PMID: 34161397] (Cell Culture, Human) Cell Culture Human
Show All 70 Publications.

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Gene Symbol BMP6