Plasminogen Antibody

Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.

Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Plasminogen Antibody Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Plasminogen [Human Plasma] (Uniprot: P00747)
• Localization: Secreted
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen from human plasma.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLG
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody has been assayed against 1.0 ug of Plasminogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H+L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
• Theoretical MW: 90.57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Plasminogen Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15075185).

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Plasminogen Antibody

• DKFZp779M0222
• EC 3.4.21
• EC 3.4.21.7
• plasmin
• Plasminogen
• Plg

Background

Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/L. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plasminogen Antibody (NB600-930)(7)

Publications using NB600-930

• Floden AM, Watt JA, Brissette CA. Borrelia burgdorferi Enolase Is a Surface-Exposed Plasminogen Binding Protein. PLoS ONE 20116(11). [PMID: 22087329]
• Basile D et al. Angiostatin matrix metalloprotease expression following ischemic acute renal failure Am J Physiol: Renal Physiology Jun 26 1905
  
  Details:
  This publication used the HRP conjugated form of this antibody (Cat# NB120-7336).
• Verma A, Brissette CA, Bowman AA et al. Leptospiral endostatin-like protein A is a bacterial cell surface receptor for human plasminogen. Infect Immun;78(5):2053-9. 2010 May. [PMID: 20160016] (ELISA, Human)
• Seling A, Siegel C, Fingerle V et al. Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen. Infect Immun;78(1):39-48. 2010 Jan. [PMID: 19858303] (ELISA, Human)
• Woodman ME, Cooley AE, Avdiushko R et al. Roles for phagocytic cells complement in controlling relapsing fever infection. J Leukoc Biol;86(3):727-736. 2009 [PMID: 19458267]
• Brissette CA, Haupt K, Barthel D et al. Borrelia burgdorferi Infection-Associated Surface Proteins ErpP, ErpA, ErpC Bind Human Plasminogen. Infect Immun;77(1):300-306. 2009 [PMID: 19001079]
• Basile DP, Fredrich K, Weihrauch D, Hattan N, Chilian WM Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am J Physiol Renal Physiol. 2004 [PMID: 15075185] (WB, Rat)

Reviews for Plasminogen Antibody (NB600-930) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Catherine Brissette ELISA Other 03/03/2011

Summary

• Application: ELISA
• Sample Tested: Recombinant protein
• Species: Other
• Lot: 6571

Primary Anitbody

• Dilution Ratio: Primary Ab Incubation Dilution Buffer: PBS, Primary Ab Dilution Ratio: 1:500, Incubation Time:1hr, Incubation Temp: RT

Details

• ELISA Plate Details: ELISA Detection Method: TMB, ELISA Sample Concentration: 10 ug/ml

reviewed by: Ashutosh Verma ELISA Other 07/02/2010

Summary

• Application: ELISA
• Sample Tested: Recombinant protein
• Species: Other
• Comments: ELISA used to measure the binding of human plasminogen to LenA protein of Leptospira.
• Pub Med ID: 20160016

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:2500

Secondary Antibody

• Secondary Description: Secondary Ab: HRP-conjugated protein G

Details

• Detection Notes: Negative Control: Gelatin, Wavelength: 450 nM
• ELISA Plate Details: ELISA Sample Concentration: 1:2500

Comments

• Comments: ELISA used to measure the binding of human plasminogen to LenA protein of Leptospira.

Bioinformatics

• Gene Symbol: PLG
• Entrez:
  • 5340(Human)
• Uniprot:
  • P00747()