Plasminogen Antibody

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Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat ...read more
Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. ...read more
Western Blot: Plasminogen Antibody [NB600-930] - Analysis using HRP conjugate of NB600-930. Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins ...read more

Product Details

Summary
Reactivity Hu, BaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Plasminogen Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Plasminogen from human plasma
Localization
Secreted
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen from human plasma.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLG
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
Application Notes
This product has been assayed against 1.0 ug of Plasminogen [Human Plasma] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Positive Control
Plasminogen Lysate (NBL1-14513)
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-930 in the following applications:

Publications
Read Publications using
NB600-930 in the following applications:

Reactivity Notes

Reactivity with Leptospira and Borrelia reported by a customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Plasminogen Antibody

  • DKFZp779M0222
  • EC 3.4.21
  • EC 3.4.21.7
  • plasmin
  • Plasminogen
  • Plg

Background

Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/L. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-930
Species: Hu, Ba
Applications: WB, ELISA

Publications for Plasminogen Antibody (NB600-930)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(2)
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Filter By Species
Human
(2)
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Showing Publications 1 - 7 of 7.
Publications using NB600-930 Applications Species
Basile D et al. Angiostatin matrix metalloprotease expression following ischemic acute renal failure Am J Physiol: Renal Physiology Jun 26 1905

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB120-7336).
Floden AM, Watt JA, Brissette CA. Borrelia burgdorferi Enolase Is a Surface-Exposed Plasminogen Binding Protein. PLoS ONE 20116(11). [PMID: 22087329]
Verma A, Brissette CA, Bowman AA et al. Leptospiral endostatin-like protein A is a bacterial cell surface receptor for human plasminogen. Infect Immun;78(5):2053-9. 2010 May. [PMID: 20160016] (ELISA, Human) ELISA Human
Seling A, Siegel C, Fingerle V et al. Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen. Infect Immun;78(1):39-48. 2010 Jan. [PMID: 19858303] (ELISA, Human) ELISA Human
Woodman ME, Cooley AE, Avdiushko R et al. Roles for phagocytic cells complement in controlling relapsing fever infection. J Leukoc Biol;86(3):727-736. 2009 [PMID: 19458267]
Brissette CA, Haupt K, Barthel D et al. Borrelia burgdorferi Infection-Associated Surface Proteins ErpP, ErpA, ErpC Bind Human Plasminogen. Infect Immun;77(1):300-306. 2009 [PMID: 19001079]
Basile, D et al. Angiostatin matrix metalloprotease expression following ischemic acute renal failure Am J Physiol: Renal Physiology 2004 [PMID: 15075185]

Details:
This citation used the HRP version of this antibody.

Reviews for Plasminogen Antibody (NB600-930) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB600-930:
Filter by Applications
ELISA
(2)
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Filter by Species
Other
(2)
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Images Ratings Applications Species Date Details
ELISA Plasminogen NB600-930
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reviewed by:
Catherine Brissette
ELISA Other 03/03/2011
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Summary

ApplicationELISA
Sample TestedRecombinant protein
SpeciesOther
Lot6571

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: PBS, Primary Ab Dilution Ratio: 1:500, Incubation Time:1hr, Incubation Temp: RT

Details

ELISA Plate DetailsELISA Detection Method: TMB, ELISA Sample Concentration: 10 ug/ml
 
reviewed by:
Ashutosh Verma
ELISA Other 07/02/2010
View

Summary

ApplicationELISA
Sample TestedRecombinant protein
SpeciesOther
CommentsELISA used to measure the binding of human plasminogen to LenA protein of Leptospira.
Pub Med ID20160016

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2500

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP-conjugated protein G

Details

Detection NotesNegative Control: Gelatin, Wavelength: 450 nM
ELISA Plate DetailsELISA Sample Concentration: 1:2500

Comments

CommentsELISA used to measure the binding of human plasminogen to LenA protein of Leptospira.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Plasminogen Antibody (NB600-930) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Catherine Brissette
03/03/2011
Application: ELISA
Species: Other

 
Ashutosh Verma
07/02/2010
Application: ELISA
Species: Other

Bioinformatics

Gene Symbol PLG
Entrez
Uniprot