Plasminogen Antibody

Images

 
Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat ...read more
Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Plasminogen Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Plasminogen [Human Plasma] (Uniprot: P00747)
Localization
Secreted
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen from human plasma.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLG
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:20000-1:100000
  • Western Blot 1:2000-1:10000
Application Notes
This antibody has been assayed against 1.0 ug of Plasminogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H+L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Theoretical MW
90.57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Plasminogen Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-930 in the following applications:

Publications
Read Publications using
NB600-930 in the following applications:

  • 2 publications
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15075185).

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Plasminogen Antibody

  • DKFZp779M0222
  • EC 3.4.21
  • EC 3.4.21.7
  • plasmin
  • Plasminogen
  • Plg

Background

Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/L. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

DSE100
Species: Hu
Applications: ELISA
1310-SE
Species: Hu
Applications: EnzAct
H00005555-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP2-76966
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47659
Species: Hu
Applications: IHC, IHC-P, WB
H00002038-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP2-23368
Species: Hu
Applications: PAGE
NBP2-01763
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-37477
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB8550
Species: Hu
Applications: CyTOF-ready, Flow, ICC, KO, WB
H00118471-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB100-96916
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DUP00
Species: Hu
Applications: ELISA
D6050
Species: Hu
Applications: ELISA
NB600-930
Species: Hu, Rt
Applications: WB, ELISA

Publications for Plasminogen Antibody (NB600-930)(7)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(2)
WB
(1)
All Applications
Filter By Species
Human
(2)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB600-930 Applications Species
Floden AM, Watt JA, Brissette CA. Borrelia burgdorferi Enolase Is a Surface-Exposed Plasminogen Binding Protein. PLoS ONE 20116(11). [PMID: 22087329]
Basile D et al. Angiostatin matrix metalloprotease expression following ischemic acute renal failure Am J Physiol: Renal Physiology Jun 26 1905

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB120-7336).
Verma A, Brissette CA, Bowman AA et al. Leptospiral endostatin-like protein A is a bacterial cell surface receptor for human plasminogen. Infect Immun;78(5):2053-9. 2010 May. [PMID: 20160016] (ELISA, Human) ELISA Human
Seling A, Siegel C, Fingerle V et al. Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen. Infect Immun;78(1):39-48. 2010 Jan. [PMID: 19858303] (ELISA, Human) ELISA Human
Woodman ME, Cooley AE, Avdiushko R et al. Roles for phagocytic cells complement in controlling relapsing fever infection. J Leukoc Biol;86(3):727-736. 2009 [PMID: 19458267]
Brissette CA, Haupt K, Barthel D et al. Borrelia burgdorferi Infection-Associated Surface Proteins ErpP, ErpA, ErpC Bind Human Plasminogen. Infect Immun;77(1):300-306. 2009 [PMID: 19001079]
Basile DP, Fredrich K, Weihrauch D, Hattan N, Chilian WM Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am J Physiol Renal Physiol. 2004 [PMID: 15075185] (WB, Rat) WB Rat

Reviews for Plasminogen Antibody (NB600-930) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB600-930:
Filter by Applications
ELISA
(2)
All Applications
Filter by Species
Other
(2)
All Species
Images Ratings Applications Species Date Details
ELISA Plasminogen NB600-930
Enlarge
5
reviewed by:
Verified Customer
ELISA Other 03/03/2011
View

Summary

ApplicationELISA
Sample TestedRecombinant protein
SpeciesOther
Lot6571
  5
reviewed by:
Verified Customer
ELISA Other 07/02/2010
View

Summary

ApplicationELISA
Sample TestedRecombinant protein
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Plasminogen Antibody (NB600-930) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Plasminogen Products

Bioinformatics Tool for Plasminogen Antibody (NB600-930)

Discover related pathways, diseases and genes to Plasminogen Antibody (NB600-930). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Plasminogen Antibody (NB600-930)

Discover more about diseases related to Plasminogen Antibody (NB600-930).
 

Pathways for Plasminogen Antibody (NB600-930)

View related products by pathway.

PTMs for Plasminogen Antibody (NB600-930)

Learn more about PTMs related to Plasminogen Antibody (NB600-930).
 

Research Areas for Plasminogen Antibody (NB600-930)

Find related products by research area.

Blogs on Plasminogen

There are no specific blogs for Plasminogen, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
03/03/2011
Application: ELISA
Species: Other

 
Verified Customer
07/02/2010
Application: ELISA
Species: Other

Bioinformatics

Gene Symbol PLG
Entrez
Uniprot