Smad3: Novus Biologicals

NBP1-77836

Smad3 [p Ser423, p Ser425] Antibody - BSA Free

(1 Review)

(13 Publications)

0.1 mg

NBP1-77836

Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody. Marker: Opal Pre-stained ladder

Mechanistic studies (I): Roflumilast prevents TGF beta 1-induced phospho-SMAD3 by the increase of PPM1A, the inhibition of reactive oxygen species (ROS) and phospho-ERK1/2-Protein Kinase A axis. Human keratinocytes were incubated for 30 min with vehicle or roflumilast (Rof, 100 nM) (A-F), the SMAD3 inhibitor (SIS3, 10uM) (A), the anti-oxidant N-acetyl-L-cysteine (NAC, 1 mM) (B, E), the PPM1A inhibitor sanguinarine (San, 3 uM) (D), the proteasome inhibitor MG132 (MG132, 5 uM) (E), the PKA inhibitor (KT5720, 2uM) (C), the ERK1/2 inhibitor (PD98059, 10uM) (F) or combinations as indicated. Next, keratinocytes were stimulated with TGF beta 1 (10 ng/ml) for 30 min and p-SMAD3 and PPM1A proteins were analysed by Western blotting. Data are shown from densitometry as the ratio of target protein compared to beta -actin of three independent experiments per condition. Results of the densitometry are presented as scatter dot blot with median and interquartile range values. P-values are based on Kruskal-Wallis test followed by the Dunn’s post-hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39223490), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Activin A facilitates TGF beta 1-induced type II receptor expression without altering TGF beta 1 receptor sensitivity in MC. a TGF beta 1 for 30 min increased TRII expression and Smad3 activation in whole cell lysate, and both were prevented by 24 h pretreatment with follistatin (n = 6). b There was no change in TRII transcript after 24 h of follistatin with or without TGF beta 1 for 30 min. c Pretreatment with an actA neutralizing antibody for 24 h inhibited acute (30 min) TGF beta 1-induced Smad3 activation and TRII upregulation (n = 6–7). These were similarly inhibited by downregulation of the actA type I receptor ALK4 with siRNA (d) (n = 3). e Pretreatment with actA (20 ng/ml) for 24 h did not affect acute TGF beta 1-induced Smad3 activation (n = 6). *, **, ****P < 0.05, 0.01, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Specific activin A inhibition attenuates TGF beta 1-induced Smad3 activation and profibrotic responses in MC. a ELISA demonstrates that TGF beta 1 (24 h) increases actA and actB secretion (n = 3) to 19.5 ng/ml and 2.5 ng/ml, representing an 8.9- and 1.06-fold induction respectively. b TGF beta 1 increases actA in whole cell lysate by 1.8-fold (n = 3). c ActA (20 ng/ml) upregulates FN (n = 3–4), CTGF (n = 4) and alpha SMA (n = 6) at 48 h. d TGF beta 1 and actA both increase Smad3 transcriptional activity; no synergistic effect is seen (n = 6–12). e An actA neutralizing antibody attenuates TGF beta 1-induced FN (n = 5–6), alpha SMA (n = 5), CTGF (n = 5–6), and Smad3 activation (n = 10–12). f ActA neutralization decreases TGF beta 1-induced Smad3 transcriptional activity at 24 h (n = 9–15), but this is not decreased by actB neutralization (n = 6) (g). h MC were stimulated with TGF beta 1 or actA for 1 h, then treated with their type I receptor inhibitor SB431542 (50 uM). Restimulation with the same ligand shows that cells become refractory to TGF beta 1, but not actA (n = 4). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Activin A neutralization inhibits renal fibrosis in TGF beta 1-overexpressing mice. a TGF beta 1 transcript is increased in mice genetically engineered to overexpress TGF beta 1 (HH) compared with wild-type mice (WT) (n = 6–7, *p ≤ 0.05). b Serum actA is elevated in wild-type and HH mice after UUO. This is decreased by treatment with a neutralizing actA antibody (anti-actA) in HH mice. c Renal actA is increased after UUO, with a greater induction in HH mice. Both are attenuated by actA neutralization. Boxed areas are shown at higher magnification immediately below. ActA increases are seen particularly in tubular epithelial cells. d Renal alpha SMA, fibronectin (FN), pSmad3 and MRTF-A are increased after UUO and this is augmented in HH kidneys. Expression of all is attenuated by actA neutralization in both WT and HH kidneys. (n = 6–9) *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test where there are > 2 groups; t-test for 2 groups Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Specific activin A inhibition attenuates TGF beta 1-induced Smad3 activation and profibrotic responses in MC. a ELISA demonstrates that TGF beta 1 (24 h) increases actA and actB secretion (n = 3) to 19.5 ng/ml and 2.5 ng/ml, representing an 8.9- and 1.06-fold induction respectively. b TGF beta 1 increases actA in whole cell lysate by 1.8-fold (n = 3). c ActA (20 ng/ml) upregulates FN (n = 3–4), CTGF (n = 4) and alpha SMA (n = 6) at 48 h. d TGF beta 1 and actA both increase Smad3 transcriptional activity; no synergistic effect is seen (n = 6–12). e An actA neutralizing antibody attenuates TGF beta 1-induced FN (n = 5–6), alpha SMA (n = 5), CTGF (n = 5–6), and Smad3 activation (n = 10–12). f ActA neutralization decreases TGF beta 1-induced Smad3 transcriptional activity at 24 h (n = 9–15), but this is not decreased by actB neutralization (n = 6) (g). h MC were stimulated with TGF beta 1 or actA for 1 h, then treated with their type I receptor inhibitor SB431542 (50 uM). Restimulation with the same ligand shows that cells become refractory to TGF beta 1, but not actA (n = 4). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Activin inhibition attenuates TGF beta 1-induced fibrotic responses and Smad3 activation in MC. Activin inhibition with follistatin (FST) decreases TGF beta 1-induced: a FN, alpha SMA and CTGF upregulation at 48 h (n = 5), b Smad3 phosphorylation (pSmad3) at 24 h (n = 5), c Smad3 nuclear translocation as assessed using eGFP-Smad3 (n = 3; 25–30 cells quantified per treatment group) at 24 h, and d Smad3 transcriptional activity at 24 h (n = 8). e Time course experiments show increases in pSmad3 occur earlier (30–60 min) with TGF beta 1 (n = 4) compared with actA (n = 4) or actB (n = 3) (18–48 h). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Impact of PAD4 deficiency on TGF‐ beta signalling. (A) Activation of the canonical and non‐canonical pathway by TGF‐ beta was examined by Western blot. TGF‐ beta stimulation (10 ng/ml) for 2 h significantly increased protein levels of pSMAD2 and pSMAD3 in CFs independently on PAD4 deficiency. TGF‐ beta failed to activate STAT3 and Akt in PAD4−/− CFs. n = 6–8. Preincubation of WT CFs with the PAD4 inhibitor Cl‐amidine for 18 h abrogated TGF‐ beta ‐induced activation of Akt (B) and subsequent GSK‐3 beta phosphorylation (C). n = 3. (D) No TGF‐ beta ‐triggered phosphorylation of GSK‐3 beta was detected in PAD4−/− CFs. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control cells for A; #p < 0.05 for B and C Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34523218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77836AF350
Alexa Fluor 405	NBP1-77836AF405
Alexa Fluor 488	NBP1-77836AF488
Alexa Fluor 532	NBP1-77836AF532
Alexa Fluor 594	NBP1-77836AF594
Alexa Fluor 647	NBP1-77836AF647
Alexa Fluor 700	NBP1-77836AF700
Alexa Fluor 750	NBP1-77836AF750
Alexa Fluor Plus 405	NBP1-77836AFP405
Alexa Fluor Plus 488	NBP1-77836AFP488
Alexa Fluor Plus 555	NBP1-77836AFP555
Alexa Fluor Plus 594	NBP1-77836AFP594
Alexa Fluor Plus 647	NBP1-77836AFP647
Alexa Fluor Plus 680	NBP1-77836AFP680
Allophycocyanin	NBP1-77836APC
Biotin	NBP1-77836B
CoraFluor 1	NBP1-77836CL1
DyLight 350	NBP1-77836UV
DyLight 405	NBP1-77836V
DyLight 488	NBP1-77836G
DyLight 550	NBP1-77836R
DyLight 594	NBP1-77836DL594
DyLight 650	NBP1-77836C
DyLight 680	NBP1-77836FR
DyLight 755	NBP1-77836IR
FITC	NBP1-77836F
HRP	NBP1-77836H
Janelia Fluor 525	NBP1-77836JF525
Janelia Fluor 549	NBP1-77836JF549
Janelia Fluor 646	NBP1-77836JF646
Janelia Fluor 669	NBP1-77836JF669
PE	NBP1-77836PE
PerCP	NBP1-77836PCP
mFluor Violet 450 SE	NBP1-77836MFV450
mFluor Violet 500 SE	NBP1-77836MFV500
mFluor Violet 610 SE	NBP1-77836MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77836SS

compare

(select up to 3 products from this page to compare)

NB100-56479

Smad3 Antibody - BSA Free

(5 Reviews)

(8 Publications)

0.1 mg

NB100-56479

Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.

Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at 0.5 ug/mL. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Effects of Sr and SB-505124 on the transforming growth factor beta (TGF beta )/SMAD family member (SMAD) signaling pathway and downstream gene transcription in bovine chondrocytes. Chondrocytes were treated with different doses of Sr (0, 0.1, 1, 10 μg/ml) with or without activin receptor-like kinase 5 (ALK5) kinase inhibitor (10 μM SB-505124). (A) Western blot analysis and relative protein expression levels of SMAD3, pSMAD3, SMAD1/5/9, pSMAD1/5/9, TGF beta , bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2). (B) mRNA expression levels of TGF beta 1, serpin family E member 1 (SERPINE1), ALK5, ALK1, and inhibitor of DNA binding 1 (ID1). All experiments were repeated at least thrice. Data are means +/- SEM (n = 3 in each group) *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35712700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Pm, Ch, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, Ch, Xp, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5848A

Alt Catalog# IMG-5848A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56479SS

compare

(select up to 3 products from this page to compare)

NBP2-67513

Smad3 [p Ser423, p Ser425] Antibody (ST0493)

100 ul

NBP2-67513

Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Phospho-Smad3(S423/S425) on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

<b>Biological Strategies Validation. </b>Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Lane 1: K562 cells, whole cell lysate, 10ug/lane, Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-Smad3(S423/S425) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 48 kDa Observed band size: 55 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds

Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on different lysates with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis on different lysates with NBP2-67513 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: A549 whole cell lysate Lane 2: A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67513 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on different lysates with NBP2-67513 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa starved overnight then treated with 10ng/mL TGF-beta 1 for 30 minutes cell lysate Lane 3: A549 cell lysate Lane 4: A549 treated with 5ng/mL TGF-beta1 for 24 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 starved overnight then treated with 10ng/mL TGF-beta 1 for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67513 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of HeLa lysates with NBP2-67513 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10 ng/ml TGF beta1 for 30 minutes cell lysate Lane 3: HeLa treated with 10 ng/ml TGF beta1 for 30 minutes cell lysate，then the membrane treated with lambda pp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67513, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP3-21516

Smad3 [p Ser423] Antibody (SR1995) - BSA Free

100 ul

NBP3-21516

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunofluorescent analysis of A549 cells treated with TGF-beta 1 , using NBP3-21516.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21516-50ul

compare

(select up to 3 products from this page to compare)

H00004088-M02

Smad3 Antibody (7F3) - Azide and BSA Free

(1 Publication)

0.1 mg

H00004088-M02

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in HeLa.

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in PC-12.

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in 293.

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in Jurkat.

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in human colon.

Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: Smad3 Antibody (7F3) [H00004088-M02] - Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

MAB4038

Smad3 Antibody (378611) [Unconjugated]

(3 Publications)

100 μg

MAB4038

CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Smad3 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 15 µg/mL Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Smad3 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 15 µg/mL Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox">

PC‑3 human prostate cancer cell line was stained with Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038, filled histogram) or isotype control antibody (Catalog # <a class=

MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Smad3 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC4038U-100UG
Alexa Fluor 405	IC4038V-100UG
Alexa Fluor 488	IC4038G-100UG
Alexa Fluor 532	FAB4038X
Alexa Fluor 594	IC4038T-100UG
Alexa Fluor 647	IC4038R-100UG
Alexa Fluor 700	IC4038N-100UG
Alexa Fluor 750	IC4038S-100UG
Alexa Fluor Plus 405	FAB4038AFP405-100UG
Alexa Fluor Plus 488	FAB4038AFP488-100UG
Alexa Fluor Plus 555	FAB4038AFP555-100UG
Alexa Fluor Plus 594	FAB4038AFP594-100UG
Alexa Fluor Plus 647	FAB4038AFP647-100UG
Alexa Fluor Plus 680	FAB4038AFP680-100UG
Allophycocyanin	FAB4038A
Biotin	FAB4038B
CoraFluor 1	FAB4038CL1
DyLight 350	FAB4038D
DyLight 405	FAB4038E
DyLight 488	FAB4038K
DyLight 550	FAB4038L
DyLight 594	FAB4038M
DyLight 650	FAB4038W
DyLight 680	FAB4038Y
DyLight 755	FAB4038Z
FITC	FAB4038F
HRP	FAB4038H
Janelia Fluor 525	FAB4038JF525
Janelia Fluor 549	FAB4038I
Janelia Fluor 646	FAB4038J
Janelia Fluor 669	FAB4038JF669
PE	FAB4038P
PerCP	FAB4038C
mFluor Violet 450 SE	FAB4038MFV450
mFluor Violet 500 SE	FAB4038MFV500
mFluor Violet 610 SE	FAB4038MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4038-SP
Carrier Free	IC4038V-100UG
Carrier Free	IC4038U-100UG
Carrier Free	IC4038T-100UG
Carrier Free	IC4038S-100UG
Carrier Free	IC4038R-100UG
Carrier Free	IC4038N-100UG
Carrier Free	IC4038G-100UG

compare

(select up to 3 products from this page to compare)

NBP3-07255

Smad3 Antibody (PCRP-SMAD3-1A2)

100 μg

NBP3-07255

Protein Array: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Smad3 Mouse Monoclonal Antibody (PCRP-Smad3-1A2).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-08287AF350
Alexa Fluor 405	NBP3-08287AF405
Alexa Fluor 488	NBP3-08287AF488
Alexa Fluor 532	NBP3-08287AF532
Alexa Fluor 594	NBP3-08287AF594
Alexa Fluor 647	NBP3-08287AF647
Alexa Fluor 700	NBP3-08287AF700
Alexa Fluor 750	NBP3-08287AF750
Alexa Fluor Plus 405	NBP3-08287AFP405
Alexa Fluor Plus 488	NBP3-08287AFP488
Alexa Fluor Plus 555	NBP3-08287AFP555
Alexa Fluor Plus 594	NBP3-08287AFP594
Alexa Fluor Plus 647	NBP3-08287AFP647
Alexa Fluor Plus 680	NBP3-08287AFP680
Allophycocyanin	NBP3-08287APC
Biotin	NBP3-08287B
CoraFluor 1	NBP3-08287CL1
DyLight 350	NBP3-08287UV
DyLight 405	NBP3-08287V
DyLight 488	NBP3-08287G
DyLight 550	NBP3-08287R
DyLight 594	NBP3-08287DL594
DyLight 650	NBP3-08287C
DyLight 680	NBP3-08287FR
DyLight 755	NBP3-08287IR
FITC	NBP3-08287F
HRP	NBP3-08287H
Janelia Fluor 525	NBP3-08287JF525
Janelia Fluor 549	NBP3-08287JF549
Janelia Fluor 646	NBP3-08287JF646
Janelia Fluor 669	NBP3-08287JF669
PE	NBP3-08287PE
PerCP	NBP3-08287PCP
mFluor Violet 450 SE	NBP3-08287MFV450
mFluor Violet 500 SE	NBP3-08287MFV500
mFluor Violet 610 SE	NBP3-08287MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-08287

compare

(select up to 3 products from this page to compare)

NBP2-20411

Smad3 Antibody - BSA Free

0.1 ml

NBP2-20411

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (NBP2-20411) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (NBP2-20411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis. <BR/>Sample: Paraffin-embedded gastric cancer. <BR/>SMAD2 / SMAD3 antibody (NBP2-20411) dilution: 1:500.<BR/> br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (NBP2-20411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, RM, Xp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-15672

Smad3 Antibody (1T1Q9)

100 ul

NBP3-15672

Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of various lysates, using Smad3 Rabbit mAb at 1:20000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.

Immunohistochemistry: Smad3 Antibody (1T1Q9) [Smad3] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] Smad3 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Smad3 Antibody (1T1Q9) [Smad3] - Immunohistochemistry analysis of paraffin-embedded Human testis using [KO Validated] Smad3 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of lysates from Rat testis, using Smad3 Rabbit mAb at 1:20000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.

<b>Genetic Strategies Validation. </b>Western Blot: Smad3 Antibody (1T1Q9) [Smad3] - Western blot analysis of lysates from wild type(WT) and Smad3 Rabbit mAb knockout (KO) HCT116(KO) cells, using Smad3 Rabbit mAb at 1:20000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP1-30284

Recombinant Human Smad3 His Protein

(1 Review)

0.1 mg

NBP1-30284

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP2-19126

Smad3 [p Thr179] Antibody - BSA Free

(2 Publications)

0.1 mg

NBP2-19126

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-57918

Smad3 Antibody - BSA Free

100 ul

NBP2-57918

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57918-25ul

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

SMAD3 Products By Gene Name

Smad3 [p Ser423, p Ser425] Antibody - BSA Free

Smad3 Antibody - BSA Free

Smad3 [p Ser423, p Ser425] Antibody (ST0493)

Smad3 [p Ser423] Antibody (SR1995) - BSA Free

Smad3 Antibody (7F3) - Azide and BSA Free

Smad3 Antibody (378611) [Unconjugated]

Smad3 Antibody (PCRP-SMAD3-1A2)

Smad3 Antibody - BSA Free

Smad3 Antibody (1T1Q9)

Recombinant Human Smad3 His Protein

Smad3 [p Thr179] Antibody - BSA Free

Smad3 Antibody - BSA Free