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Serpin E1/PAI-1 Antibody

 (10 Publications)
 (2 Reviews)
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Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.
Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.
Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
NBP1-19773AF488
Alexa Fluor 647
NBP1-19773AF647
Biotin
NBP1-19773B
DyLight 350
NBP1-19773UV
DyLight 405
NBP1-19773V
DyLight 488
NBP1-19773G
DyLight 550
NBP1-19773R
DyLight 650
NBP1-19773C
DyLight 680
NBP1-19773FR
DyLight 755
NBP1-19773IR
FITC
NBP1-19773F
HRP
NBP1-19773H
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Serpin E1/PAI-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]
Localization
Secreted.
Specificity
PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SERPINE1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in ELISA reported in scientific literature (PMID 28132883).
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-19773 in the following applications:
Publications
Read Publications using
NBP1-19773 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin E1/PAI-1 Antibody

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
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Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Paraffin-embedded colon carcinoma. tPA antibody dilution: 1:250.
Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody diluted at 1:2000.
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u-Plasminogen Activator (uPA)/Urokinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human u-Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
u-Plasminogen Activator (uPA)/Urokinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human u-Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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PRAP1 Antibody
NBP1-80633
Immunohistochemistry-Paraffin: PRAP1 Antibody [NBP1-80633] - Staining of human duodenum shows strong positivity in a subset of cells.
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TNF-alpha
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H00005555-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
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NBP1-58268
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Serpin E1/PAI-1
Antibody
NBP1-19773
Serpin E1/PAI-1 Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Serpin E1/PAI-1 Antibody (NBP1-19773)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-19773 Applications Species
Mallikarjuna P, Sitaram RT, Landstrom M, Ljungberg B. VHL status regulates transforming growth factor-b signaling pathways in renal cell carcinoma. Oncotarget Mar 27 2018 [PMID: 29662646] (WB, Human) WB Human
Gerenu G, Martisova E, Ferrero H et al. Modulation of BDNF cleavage by plasminogen-activator inhibitor-1 contributes to Alzheimer's neuropathology and cognitive deficits Biochim. Biophys. Acta Jan 26 2017 [PMID: 28132883] (ELISA, Mouse) ELISA Mouse
Sitaram RT, Mallikarjuna P, Landstrom M, Ljungberg B. Transforming growth factor-beta promotes aggressiveness and invasion of clear cell renal cell carcinoma. Oncotarget. May 4 2016 [PMID: 27166254] (WB, Human) WB Human
Lee Ji-Hye, Oh Mee-Hye, Park Jae-Seok et al. Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis. Korean J Intern Med. 2014 Feb 27 [PMID: 24648800] (Human) Human
Longchamp A, Alonso F, Dubuis C et al. The use of external mesh reinforcement to reduce intimal hyperplasia and preserve the structure of human saphenous veins. Biomaterials 2014 Mar 1 [PMID: 24429385] (WB, Human) WB Human
Dubuis C, May L, Alonso F et al. Atorvastatin-Loaded Hydrogel Affects theSmooth Muscle Cells of Human Veins. J Pharmacol Exp Ther. 2013 Sep 26 [PMID: 24071735] (WB, ICC/IF, Human) WB, ICC/IF Human
Berard X, Deglise S, Alonso F et al. Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins. J Vasc Surg 2013 Jan 23 [PMID: 23351647] (WB, Human) WB Human
Cao Y, Szabolcs A, Dutta SK et al. Neuropilin-1 mediates divergent R-Smad signaling and the myofibroblast phenotype. J Biol Chem 2010 Oct 8 [PMID: 20675371] (WB, Human) WB Human
Lee JH, Oh MH, Park JS et al. Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis. Korean J Intern Med 2014 (IHC-P, Human) IHC-P Human
Liao YC, Wang YS, Guo YC et al. Let-7g improves multiple endothelial functions through targeting TGF-beta and SIRT-1 signaling. J Am Coll Cardiol. 2013 Nov 14 [PMID: 24291274] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse

Reviews for Serpin E1/PAI-1 Antibody (NBP1-19773) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-19773:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot Serpin E1/PAI-1 NBP1-19773
Enlarge
reviewed by:
Pramod Mallikarjuna
 WB Human 11/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedHEK 293 cell line vs. HEK 293 cell line treated with TGF-beta
SpeciesHuman

Blocking

Blocking DetailsLi-Cor Odyssey blocking buffer.

Primary Anitbody

Dilution Ratio1:1000 in TBST, Incubated overnight, 4 degrees overnight.

Secondary Antibody

Secondary DescriptionIRDye® 800CW Goat anti-Rabbit IgG (H + L)
Secondary Manufacturer Cat#licor P/N 925-32211
Secondary Concentration1:10000

Details

Detection NotesOdyssey CLx, 84 µm, auto exposure
Western Blot Serpin E1/PAI-1 NBP1-19773
Enlarge
reviewed by:
Pramod Mallikarjuna
 WB Human 06/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedRenal Cell Carcinoma tissues
SpeciesHuman

Blocking

Blocking DetailsLi-Cor odyssey blocking buffer

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionLi-Cor IRDye 800CW Goat anti-Rabbit
Secondary Manufacturer Cat#926-32211
Secondary Concentration1:10000

Product General Protocols

View specific protocols for Serpin E1/PAI-1 Antibody (NBP1-19773): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serpin E1/PAI-1 Antibody (NBP1-19773) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-19773AF488
Alexa Fluor 647 Labeled NBP1-19773AF647
Biotin Labeled NBP1-19773B
DyLight 350 Labeled NBP1-19773UV
DyLight 405 Labeled NBP1-19773V
DyLight 488 Labeled NBP1-19773G
DyLight 550 Labeled NBP1-19773R
DyLight 650 Labeled NBP1-19773C
DyLight 680 Labeled NBP1-19773FR
DyLight 755 Labeled NBP1-19773IR
HRP Labeled NBP1-19773H
Learn about Custom Antibody Labeling

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Product PDFs

  Western Blot Protocol
  IHC Protocol
  ICC/IF Protocol
  SDS
  PDF Datasheet
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Recent Reviews

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Western Blot Serpin E1/PAI-1 NBP1-19773
Enlarge
 Pramod Mallikarjuna
11/26/2015
Application: WB
Species: Human
read this review
Western Blot Serpin E1/PAI-1 NBP1-19773
Enlarge
 Pramod Mallikarjuna
06/24/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SERPINE1
Entrez
Uniprot