Serpin E1/PAI-1 Antibody

Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, andI2-actin in A498 cells after treatment with TGF-i2 at given point of time. Image collected and cropped by Cite Ab from the following publication (VHL status regulates transforming growth factor-β signaling pathways in renal cell carcinoma. Oncotarget (2018)) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.

Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.

Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Immunoblots showing protein expression of pVHL, ALK5, pSMAD2/3, PAI-1, andI2-actin after transfection of indicated vectors in A498 cells followed by TGF-i2 treatment for 6 hours. Image collected and cropped by Cite Ab from the following publication (VHL status regulates transforming growth factor-β signaling pathways in renal cell carcinoma. Oncotarget (2018)) licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Serpin E1/PAI-1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]
• Localization: Secreted.
• Specificity: PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin E1/PAI-1 Antibody

• Endothelial plasminogen activator inhibitor
• Nexin
• PAI1
• PAI-1
• PAI1PAI-1
• PAISerpin E1
• PLANH1
• PLANH1plasminogen activator inhibitor 1
• serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
• Serpin E1
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin E1/PAI-1 Antibody (NBP1-19773)(19)

Publications using NBP1-19773

• Sun H, Pratt RE, Hodgkinson CP, Dzau VJ Sequential paracrine mechanisms are necessary for the therapeutic benefits of stem cell therapy Am J Physiol Cell Physiol Oct 7 2020 [PMID: 33026832]
• Lee YK, Kwon SM, Lee EB et al. Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis Cancers Sep 15 2020 [PMID: 32942643] (WB, Human)
• Ishiwata S, Iizuka H, Sonoda H et al. Upregulated miR-224-5p suppresses osteoblast differentiation by increasing the expression of Pai-1 in the lumbar spine of a rat model of congenital kyphoscoliosis Mol. Cell. Biochem. Aug 3 2020 [PMID: 32748313] (WB)
• Kimura N, Nakagami G, Minematsu T, Sanada H Non-invasive detection of local tissue responses to predict pressure ulcer development in mouse models J Tissue Viability Nov 4 2019 [PMID: 31757582] (IHC, IHC-P, Mouse)
• LaRocca TJ, Mariani A, Watkins LR et al. TDP-43 knockdown causes innate immune activation via protein kinase R in astrocytes Neurobiol. Dis. Jun 20 2019 [PMID: 31229690] (ICC/IF, Rat)
• Delfarah A, Parrish S et al. Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cells. J Biol Chem May 7 2019 [PMID: 31138644] (WB, Human)
• Zhong Y, Zhang Y, Xu J et al. Low-Intensity Focused Ultrasound-Responsive Phase-Transitional Nanoparticles for Thrombolysis without Vascular Damage: A Synergistic Nonpharmaceutical Strategy ACS Nano Mar 26 2019 [PMID: 30855938] (IHC, Rat)
• Cheng Q, Tong F, Shen Y et al. Achyranthes bidentata polypeptide k improves long-term neurological outcomes through reducing downstream microvascular thrombosis in experimental ischemic stroke. Lepr Rev. Mar 1 2018 [PMID: 30414726] (IHC, WB, Rat)
• PriYadarshini R, Hussain M, Attri P et al. BLM Potentiates c-Jun Degradation and Alters Its Function as an Oncogenic Transcription Factor. Cell Rep. Jul 24 2018 [PMID: 30044990] (WB)
• Hiebert P, Wietecha MS, Cangkrama M et al. Nrf2-Mediated Fibroblast Reprogramming Drives Cellular Senescence by Targeting the Matrisome. Dev. Cell. Jul 16 2018 [PMID: 30016619] (Mouse)
• Mallikarjuna P, Sitaram RT, Landstrom M, Ljungberg B. VHL status regulates transforming growth factor-b signaling pathways in renal cell carcinoma. Oncotarget Mar 27 2018 [PMID: 29662646] (WB, Human)
• Gerenu G, Martisova E, Ferrero H et al. Modulation of BDNF cleavage by plasminogen-activator inhibitor-1 contributes to Alzheimer's neuropathology and cognitive deficits Biochim. Biophys. Acta Jan 26 2017 [PMID: 28132883] (ELISA, Mouse)
• Sitaram RT, Mallikarjuna P, Landstrom M, Ljungberg B. Transforming growth factor-beta promotes aggressiveness and invasion of clear cell renal cell carcinoma. Oncotarget. May 4 2016 [PMID: 27166254] (WB, Human)
• Lee Ji-Hye, Oh Mee-Hye, Park Jae-Seok et al. Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis. Korean J Intern Med. 2014 Feb 27 [PMID: 24648800] (Human)
• Longchamp A, Alonso F, Dubuis C et al. The use of external mesh reinforcement to reduce intimal hyperplasia and preserve the structure of human saphenous veins. Biomaterials 2014 Mar 1 [PMID: 24429385] (WB, Human)
• Liao YC, Wang YS, Guo YC et al. Let-7g improves multiple endothelial functions through targeting TGF-beta and SIRT-1 signaling. J Am Coll Cardiol. 2013 Nov 14 [PMID: 24291274] (IHC-Fr, ICC/IF, Mouse)
• Dubuis C, May L, Alonso F et al. Atorvastatin-Loaded Hydrogel Affects theSmooth Muscle Cells of Human Veins. J Pharmacol Exp Ther. 2013 Sep 26 [PMID: 24071735] (WB, ICC/IF, Human)
• Berard X, Deglise S, Alonso F et al. Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins. J Vasc Surg 2013 Jan 23 [PMID: 23351647] (WB, Human)
• Cao Y, Szabolcs A, Dutta SK et al. Neuropilin-1 mediates divergent R-Smad signaling and the myofibroblast phenotype. J Biol Chem 2010 Oct 8 [PMID: 20675371] (WB, Human)

Reviews for Serpin E1/PAI-1 Antibody (NBP1-19773) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Pramod Mallikarjuna WB Human 11/26/2015

Summary

• Application: Western Blot
• Sample Tested: HEK 293 cell line vs. HEK 293 cell line treated with TGF-beta
• Species: Human

Blocking

• Blocking Details: Li-Cor Odyssey blocking buffer.

Primary Anitbody

• Dilution Ratio: 1:1000 in TBST, Incubated overnight, 4 degrees overnight.

Secondary Antibody

• Secondary Description: IRDye® 800CW Goat anti-Rabbit IgG (H + L)
• Secondary Manufacturer Cat#: licor P/N 925-32211
• Secondary Concentration: 1:10000

Details

• Detection Notes: Odyssey CLx, 84 µm, auto exposure

reviewed by: Pramod Mallikarjuna WB Human 06/24/2014

Summary

• Application: Western Blot
• Sample Tested: Renal Cell Carcinoma tissues
• Species: Human

Blocking

• Blocking Details: Li-Cor odyssey blocking buffer

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Li-Cor IRDye 800CW Goat anti-Rabbit
• Secondary Manufacturer Cat#: 926-32211
• Secondary Concentration: 1:10000

Bioinformatics

• Gene Symbol: SERPINE1
• Entrez:
  • 5054(Human)
  • 18787(Mouse)
  • 24617(Rat)
• Uniprot:
  • P05121()