SMAD1/5/8/9 Antibody - BSA Free

Western Blot: SMAD1/5/8/9 Antibody [NB600-962] - Analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMAD1/5/8/9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMAD1/5/8/9 Antibody - BSA Free (NB600-962) is a polyclonal antibody validated for use in WB. Anti-SMAD1/5/8/9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
• Localization: Cytoplasmic and Nuclear
• Specificity: The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: Western Blot: Detects a band of approximately 52 kDa.

Reactivity Notes

Predicted to react with Chicken, Chimpanzee, Invertebrates, Mammals, Porcine, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SMAD1/5/8/9 Antibody - BSA Free

• BSP1
• BSP-1
• hSMAD1
• JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
• MAD homolog 1
• MADH1JV41
• MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
• Mad-related protein 1
• mothers against decapentaplegic homolog 1
• Mothers against DPP homolog 1
• SMAD family member 1SMAD 1
• SMAD, mothers against DPP homolog 1
• Smad1
• TGF-beta signaling protein 1
• transforming growth factor-beta signaling protein 1

Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAD1/5/8/9 Antibody (NB600-962)(1)

Publication using NB600-962

• Yoshihara T, Dobashi S, Takaragawa M, Naito H Effect of losartan treatment on Smad signaling and recovery from hindlimb unloading-induced soleus muscle atrophy in female rats European journal of pharmacology 2022-09-15 [PMID: 35988789] (WB, Rat)

Bioinformatics

• Gene Symbol: SMAD1
• Entrez:
  • 17129(Mouse)
  • 59328(Rat)