SMAD1/5/8/9 Antibody


Western Blot: SMAD1/5/8/9 Antibody [NB600-962] - analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
0.5 mg/ml

Order Details

SMAD1/5/8/9 Antibody Summary

This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Cytoplasmic and Nuclear
The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
200 ul PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 0.5 - 2 ug/ml
Application Notes
Western Blot: Detects a band of approximately 52 kDa.

Reactivity Notes

Predicted to react with Chicken, Chimpanzee, Invertebrates, Mammals, Pig, Xenopus and Zebrafish.

Alternate Names for SMAD1/5/8/9 Antibody

  • BSP1
  • BSP-1
  • hSMAD1
  • JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
  • MAD homolog 1
  • MADH1JV41
  • MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
  • Mad-related protein 1
  • mothers against decapentaplegic homolog 1
  • Mothers against DPP homolog 1
  • SMAD family member 1SMAD 1
  • SMAD, mothers against DPP homolog 1
  • Smad1
  • TGF-beta signaling protein 1
  • transforming growth factor-beta signaling protein 1


SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: IHC, ICC
Species: Hu, Mu, Rt, Pm, Sh
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt
Applications: WB
Supplier Logo

Publications for SMAD1/5/8/9 Antibody (NB600-962) (0)

There are no publications for SMAD1/5/8/9 Antibody (NB600-962).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SMAD1/5/8/9 Antibody (NB600-962) (0)

There are no reviews for SMAD1/5/8/9 Antibody (NB600-962). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SMAD1/5/8/9 Antibody (NB600-962) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional SMAD1/5/8/9 Antibody Products

SMAD1/5/8/9 NB600-962

Related Products by Gene

Bioinformatics Tool for SMAD1/5/8/9 Antibody (NB600-962)

Discover related pathways, diseases and genes to SMAD1/5/8/9 Antibody (NB600-962). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SMAD1/5/8/9 Antibody (NB600-962)

Discover more about diseases related to SMAD1/5/8/9 Antibody (NB600-962).

Pathways for SMAD1/5/8/9 Antibody (NB600-962)

View related products by pathway.

PTMs for SMAD1/5/8/9 Antibody (NB600-962)

Learn more about PTMs related to SMAD1/5/8/9 Antibody (NB600-962).

Blogs on SMAD1/5/8/9

There are no specific blogs for SMAD1/5/8/9, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol SMAD1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-962 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought