ALK-1 Antibody

Images

 
There are currently no images for ALK-1 Antibody (AF370).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

ALK-1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALK‑1
Asp22-Gln118
Accession # P37023
Specificity
Detects human ALK-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse ALK-1 is observed and less than 1% cross-reactivity with recombinant human (rh) Activin RIA and rhActivin RIB is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACVRL1
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunoprecipitation
Reviewed Applications
Read 1 Review rated 5
using
AF370 in the following applications:

Publications
Read Publications using
AF370 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALK-1 Antibody

  • activin A receptor type II-like 1
  • activin A receptor, type II-like kinase 1
  • Activin receptor-like kinase 1
  • ACVRL1
  • ACVRLK1ORW2
  • ALK1
  • ALK-1
  • ALK1TGF-B superfamily receptor type I
  • EC 2.7.11
  • HHT
  • HHT2EC 2.7.11.30
  • serine/threonine-protein kinase receptor R3
  • SKR3
  • TSR-I

Background

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP1-85533
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56443
Species: Hu, Mu, Rt
Applications: WB
NBP2-61845
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NBP2-46157
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-37624
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-24951
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
AF637
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for ALK-1 Antibody (AF370)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, IHC-Fr, Immunoprecipitation, Neutralization, Surface Plasmon Resonance, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Neutralization
(1)
Surface Plasmon Resonance
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF370 Applications Species
Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension. PLoS ONE, 2014;9(6):e100310. 2014 [PMID: 24956016] (Western Blot, Human) Western Blot Human
van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting. J. Biol. Chem., 2012;287(22):18551-61. 2012 [PMID: 22493445] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Ricard N, Bidart M, Mallet C Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations. Blood, 2010;116(9):1604-12. 2010 [PMID: 20501893] (Flow Cytometry, Human) Flow Cytometry Human
Mahmoud M, Borthwick GM, Hislop AA, Arthur HM Endoglin and activin receptor-like-kinase 1 are co-expressed in the distal vessels of the lung: implications for two familial vascular dysplasias, HHT and PAH. Lab. Invest., 2009;89(1):15-25. 2009 [PMID: 19015642] (IHC-Fr, Mouse) IHC-Fr Mouse
Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production. Am. J. Pathol., 2008;172(4):1127-40. 2008 [PMID: 18349127] (Western Blot, Human) Western Blot Human
Korff T, Aufgebauer K, Hecker M Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response. Circulation, 2007;116(20):2288-97. 2007 [PMID: 17967972] (Neutralization, Human) Neutralization Human
David L, Mallet C, Vailhe B, Lamouille S, Feige JJ, Bailly S Activin receptor-like kinase 1 inhibits human microvascular endothelial cell migration: potential roles for JNK and ERK. J. Cell. Physiol., 2007;213(2):484-9. 2007 [PMID: 17620321] (Western Blot, Human) Western Blot Human
Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Eur. Respir. J., 2007;29(6):1094-104. 2007 [PMID: 17392319] (Western Blot, Rat) Western Blot Rat
David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells. Blood, 2007;109(5):1953-61. 2007 [PMID: 17068149] (Immunoprecipitation, Human) Immunoprecipitation Human

Review for ALK-1 Antibody (AF370) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF370:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ALK-1 AF370
Enlarge
5
reviewed by:
Anonymous
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedHuman lung cancer line H1299,NCI-H1650 human non-small cell lung cancer cell line
SpeciesHuman
LotDLU0115101

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ALK-1 Antibody (AF370) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF307
Biotin BAF370

Additional ALK-1 Products

Bioinformatics Tool for ALK-1 Antibody (AF370)

Discover related pathways, diseases and genes to ALK-1 Antibody (AF370). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ALK-1 Antibody (AF370)

Discover more about diseases related to ALK-1 Antibody (AF370).
 

Pathways for ALK-1 Antibody (AF370)

View related products by pathway.

PTMs for ALK-1 Antibody (AF370)

Learn more about PTMs related to ALK-1 Antibody (AF370).

Blogs on ALK-1

There are no specific blogs for ALK-1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACVRL1
Uniprot