ALK-1 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ALK-1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ALK‑1
  Asp22-Gln118
  Accession # P37023
• Specificity: Detects human ALK-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse ALK-1 is observed and less than 1% cross-reactivity with recombinant human (rh) Activin RIA and rhActivin RIB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVRL1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALK-1 Antibody

• activin A receptor type II-like 1
• activin A receptor, type II-like kinase 1
• Activin receptor-like kinase 1
• ACVRL1
• ACVRLK1ORW2
• ALK1
• ALK-1
• ALK1TGF-B superfamily receptor type I
• EC 2.7.11
• HHT
• HHT2EC 2.7.11.30
• serine/threonine-protein kinase receptor R3
• SKR3
• TSR-I

Background

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
2. ten Dijke, P. et al. (1994) Science 264:101.
3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALK-1 Antibody (AF370)(13)

Publications using AF370

• AB Jaykumar, S Plumber, DM Barry, D Binns, C Wichaidit, M Grzemska, S Earnest, EJ Goldsmith, O Cleaver, MH Cobb WNK1 collaborates with TGF-beta in endothelial cell junction turnover and angiogenesis Proceedings of the National Academy of Sciences of the United States of America, 2022-07-22;119(30):e2203743119. 2022-07-22 [PMID: 35867836] (Western Blot, Human)
• B Tao, JR Kraehling, S Ghaffari, CM Ramirez, S Lee, JW Fowler, WL Lee, C Fernandez-, A Eichmann, WC Sessa BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells J Biol Chem, 2020-10-23;0(0):. 2020-10-23 [PMID: 33097593] (ICC, Human)
• T Toyonaga, EC Steinbach, BP Keith, JB Barrow, MR Schaner, EA Wolber, C Beasley, J Huling, Y Wang, NL Allbritton, N Chaumont, TS Sadiq, MJ Koruda, A Jain, MD Long, EL Barnes, HH Herfarth, KL Isaacs, JJ Hansen, MT Shanahan, R Rahbar, TS Furey, P Sethupathy, SZ Sheikh Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier Integrity in Patients with Crohn&#039;s disease Cell Mol Gastroenterol Hepatol, 2020-06-16;0(0):. 2020-06-16 [PMID: 32561494] (Western Blot, Human)
• C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020-02-10;0(0):. 2020-02-10 [PMID: 32042114] (Western Blot, Transgenic Mouse)
• Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension. PLoS ONE, 2014-06-23;9(6):e100310. 2014-06-23 [PMID: 24956016] (Western Blot, Human)
• van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting. J. Biol. Chem., 2012-04-05;287(22):18551-61. 2012-04-05 [PMID: 22493445] (Surface Plasmon Resonance, Human)
• Ricard N, Bidart M, Mallet C Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations. Blood, 2010-05-25;116(9):1604-12. 2010-05-25 [PMID: 20501893] (Flow Cytometry, Human)
• Mahmoud M, Borthwick GM, Hislop AA, Arthur HM Endoglin and activin receptor-like-kinase 1 are co-expressed in the distal vessels of the lung: implications for two familial vascular dysplasias, HHT and PAH. Lab. Invest., 2008-11-17;89(1):15-25. 2008-11-17 [PMID: 19015642] (IHC-Fr, Mouse)
• Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production. Am. J. Pathol., 2008-03-18;172(4):1127-40. 2008-03-18 [PMID: 18349127] (Flow Cytometry, Western Blot, Human)
• Korff T, Aufgebauer K, Hecker M Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response. Circulation, 2007-10-29;116(20):2288-97. 2007-10-29 [PMID: 17967972] (Neutralization, Human)
• David L, Mallet C, Vailhe B, Lamouille S, Feige JJ, Bailly S Activin receptor-like kinase 1 inhibits human microvascular endothelial cell migration: potential roles for JNK and ERK. J. Cell. Physiol., 2007-11-01;213(2):484-9. 2007-11-01 [PMID: 17620321] (Western Blot, Human)
• Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Eur. Respir. J., 2007-03-28;29(6):1094-104. 2007-03-28 [PMID: 17392319] (Western Blot, Rat)
• David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells. Blood, 2006-10-26;109(5):1953-61. 2006-10-26 [PMID: 17068149] (Immunoprecipitation, Human)

Review for ALK-1 Antibody (AF370) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lei Nie WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: Human lung cancer line H1299,NCI-H1650 human non-small cell lung cancer cell line
• Species: Human
• Lot: DLU0115101

Bioinformatics

• Gene Symbol: ACVRL1
• Uniprot:
  • P37023()