Human SLPI Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human SLPI Quantikine ELISA Kit Summary

• Background: The Quantikine Human SLPI Immunoassay is a 4.5 hour solid phase ELISA designed to measure SLPI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human SLPI and antibodies raised against the recombinant factor. The kit has been shown to accurately quantitate recombinant human SLPI. Results obtained using natural human SLPI showed linear c...urves that were parallel to the curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SLPI.
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• Specificity: Natural and recombinant human SLPI
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SLPI

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human SLPI Quantikine ELISA Kit

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

Secretory leukocyte protease inhibitor (SLPI) is found in various secretory fluids including parotid secretions, bronchial, nasal, cervical mucous, and seminal fluid. SLPI is a potent inhibitor of many proteolytic enzymes including the neutrophil proteases, elastase, cathepsin G, chymotrypsin, and trypsin.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for SLPI (DPI00)(37)

Publications using DPI00

• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• Knipping, K;Kartaram, SW;Teunis, M;Zuithoff, NPA;Buurman, N;M'Rabet, L;van Norren, K;Witkamp, R;Pieters, R;Garssen, J; Salivary concentrations of secretory leukocyte protease inhibitor and matrix metallopeptidase-9 following a single bout of exercise are associated with intensity and hydration status PloS one 2023-11-22 [PMID: 37992002] (Human)
• Flerlage, T;Crawford, JC;Allen, EK;Severns, D;Tan, S;Surman, S;Ridout, G;Novak, T;Randolph, A;West, AN;Thomas, PG; Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome Nature communications 2023-06-30 [PMID: 37391405] (Human)
• Y Miyazaki, T Goto, X Li, K Nakayama, K Okasho, M Takeda, K Mizuno, H Kimura, M Uegaki, T Sumiyoshi, Y Teramoto, S Akamatsu, T Kobayashi, O Ogawa, T Inoue Up-regulation of secretory leukocyte protease inhibitor in human samples might have a potential role of predicting prostate cancer recurrence and progression after surgery and hormonal therapy Cancer Medicine, 2022-08-13;12(3):3328-3342. 2022-08-13 [PMID: 36812122] (Human)
• KT Sawicki, DR Nannini, SJ Bielinski, NB Larson, DM Lloyd-Jone, B Psaty, KD Taylor, SJ Shah, LJ Rasmussen-, JT Wilkins, EM McNally, RB Patel Secretory leukocyte protease inhibitor and risk of heart failure in the Multi-Ethnic Study of Atherosclerosis Scientific Reports, 2023-01-12;13(1):604. 2023-01-12 [PMID: 36635319] (Human)
• DL Frey, C Bridson, S Dittrich, SY Graeber, M Stahl, S Wege, F Herth, O Sommerburg, C Schultz, A Dalpke, MA Mall, S Boutin Changes in Microbiome Dominance Are Associated With Declining Lung Function and Fluctuating Inflammation in People With Cystic Fibrosis Frontiers in Microbiology, 2022-05-13;13(0):885822. 2022-05-13 [PMID: 35633718] (Human)
• O Sibila, L Perea, N Albacar, J Moisés, T Cruz, N Mendoza, B Solarat, G Lledó, G Espinosa, JA Barberà, JR Badia, A Agustí, J Sellarés, R Faner Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital discharge Respiratory Research, 2022-02-21;23(1):37. 2022-02-21 [PMID: 35189887] (Human)
• BL Dickey, B Sirak, L Martin-Gom, RR Reich, M Abrahamsen, K Isaacs-Sor, CH Chung, AR Giuliano Oral secretory leukocyte protease inhibitor (SLPI): Associations with oropharyngeal cancer and treatment outcome PLoS ONE, 2021-07-02;16(7):e0254161. 2021-07-02 [PMID: 34214131] (Human)
• T Samejima, T Nagamatsu, N Akiba, T Fujii, S Sayama, K Kawana, A Taguchi, K Kumasawa, T Iriyama, Y Osuga, T Fujii Secretory leukocyte protease inhibitor and progranulin as possible regulators of cervical remodeling in pregnancy J Reprod Immunol, 2020-11-01;143(0):103241. 2020-11-01 [PMID: 33157500] (Human)
• A Stanton, C Mowbray, M Lanz, K Brown, P Hilton, A Tyson-Capp, RS Pickard, ASM Ali, J Hall Topical Estrogen Treatment Augments the Vaginal Response to Escherichia coli Flagellin Sci Rep, 2020-05-21;10(1):8473. 2020-05-21 [PMID: 32439855] (Human)
• HJ Whitwell, J Worthingto, O Blyuss, A Gentry-Mah, A Ryan, R Gunu, J Kalsi, U Menon, I Jacobs, A Zaikin, JF Timms Improved early detection of ovarian cancer using longitudinal multimarker models Br. J. Cancer, 2020-01-15;0(0):. 2020-01-15 [PMID: 31937926] (Human)
• L Averdunk, C Fitzner, T Levkovich, DE Leaf, M Sobotta, J Vieten, A Ochi, G Moeckel, G Marx, C Stoppe Secretory Leukocyte Protease Inhibitor (SLPI)-A Novel Predictive Biomarker of Acute Kidney Injury after Cardiac Surgery: A Prospective Observational Study J Clin Med, 2019-11-09;8(11):. 2019-11-09 [PMID: 31717603] (Human)
• K Kongpol, N Nernpermpi, E Prompunt, S Kumphune Endothelial-Cell-Derived Human Secretory Leukocyte Protease Inhibitor (SLPI) Protects Cardiomyocytes against Ischemia/Reperfusion Injury Biomolecules, 2019-10-31;9(11):. 2019-10-31 [PMID: 31683729] (Human)
• Smith-McCune K, Chen J, Greenblatt R, Shanmugasundaram U, Shacklett B, Hilton J, Johnson B, Irwin J, Giudice L Unexpected Inflammatory Effects of Intravaginal Gels (Universal Placebo Gel and Nonoxynol-9) on the Upper Female Reproductive Tract: A Randomized Crossover Study. PLoS ONE, 2015-07-15;10(7):e0129769. 2015-07-15 [PMID: 26177352] (Human)
• Raundhal M, Morse C, Khare A, Oriss T, Milosevic J, Trudeau J, Huff R, Pilewski J, Holguin F, Kolls J, Wenzel S, Ray P, Ray A High IFN-gamma and low SLPI mark severe asthma in mice and humans. J Clin Invest, 2015-06-29;125(8):3037-50. 2015-06-29 [PMID: 26121748] (Human)
• Pahlman L, Jogi A, Gram M, Mori M, Egesten A Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1. BMC Pulm Med, 2015-03-07;15(0):19. 2015-03-07 [PMID: 25851169] (Human)
• Wilflingseder J, Sunzenauer J, Toronyi E, Heinzel A, Kainz A, Mayer B, Perco P, Telkes G, Langer R, Oberbauer R Molecular pathogenesis of post-transplant acute kidney injury: assessment of whole-genome mRNA and miRNA profiles. PLoS ONE, 2014-08-05;9(8):e104164. 2014-08-05 [PMID: 25093671] (Human)
• Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood, 2013-12-18;123(8):1239-49. 2013-12-18 [PMID: 24352879] (Human)
• Suh HS, Choi N, Tarassishin L, Lee SC Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12). PLoS ONE, 2012-04-11;7(4):e35115. 2012-04-11 [PMID: 22509390] (Human)
• Tse KP, Wu CS, Hsueh C The relationship between secretory leukocyte protease inhibitor expression and Epstein-Barr virus status among patients with nasopharyngeal carcinoma. Anticancer Res., 2012-04-01;32(4):1299-307. 2012-04-01 [PMID: 22493362] (Human)
• Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma. Am. J. Pathol., 2011-06-01;178(6):2866-78. 2011-06-01 [PMID: 21641406] (Human)
• Keller MJ, Madan RP, Torres NM A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel. PLoS ONE, 2011-01-25;6(1):e16475. 2011-01-25 [PMID: 21283552] (Human)
• Cheng WL, Wang CS, Huang YH, Liang Y, Lin PY, Hsueh C, Wu YC, Chen WJ, Yu CJ, Lin SR, Lin KH Overexpression of a secretory leukocyte protease inhibitor in human gastric cancer. Int. J. Cancer, 2008-10-15;123(8):1787-96. 2008-10-15 [PMID: 18688858] (Human)
• Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines. AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71. 2008-05-01 [PMID: 18462081] (Human)
• Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients. Mol. Cell Proteomics, 2008-04-03;7(10):1974-82. 2008-04-03 [PMID: 18388126] (Human)
• Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils. J. Leukoc. Biol., 2008-02-19;83(5):1155-64. 2008-02-19 [PMID: 18285402] (Human)
• Cummins JE, Denniston M, Mayer KH, Pickard R, Novak RM, Graham P, Gurwith M, Orelind K, Ackers ML, Dezzutti CS Mucosal innate immune factors in secretions from high-risk individuals immunized with a bivalent gp120 vaccine. AIDS Res. Hum. Retroviruses, 2007-05-01;23(5):748-54. 2007-05-01 [PMID: 17531002] (Human)
• Keller MJ, Guzman E, Hazrati E, Kasowitz A, Cheshenko N, Wallenstein S, Cole AL, Cole AM, Profy AT, Wira CR, Hogarty K, Herold BC PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity. AIDS, 2007-02-19;21(4):467-76. 2007-02-19 [PMID: 17301565] (Human)
• Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma. Clin. Cancer Res., 2007-01-15;13(2):458-66. 2007-01-15 [PMID: 17255266] (Human)
• Into T, Inomata M, Kanno Y, Matsuyama T, Machigashira M, Izumi Y, Imamura T, Nakashima M, Noguchi T, Matsushita K Arginine-specific gingipains from Porphyromonas gingivalis deprive protective functions of secretory leucocyte protease inhibitor in periodontal tissue. Clin. Exp. Immunol., 2006-09-01;145(3):545-54. 2006-09-01 [PMID: 16907925] (Human)
• Simpson JL, Scott RJ, Boyle MJ, Gibson PG Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma. Am. J. Respir. Crit. Care Med., 2005-05-18;172(5):559-65. 2005-05-18 [PMID: 15901607] (Human)
• Chattopadhyay A, Gray LR, Patton LL, Caplan DJ, Slade GD, Tien HC, Shugars DC Salivary secretory leukocyte protease inhibitor and oral candidiasis in human immunodeficiency virus type 1-infected persons. Infect. Immun., 2004-04-01;72(4):1956-63. 2004-04-01 [PMID: 15039315] (Human)
• Kamal AM, Corrigan CJ, Tetley TD, Alaghband-Zadeh J, Smith SF Effect of fluticasone on the elastase:antielastase profile of the normal lung. Eur. J. Clin. Invest., 2002-09-01;32(9):713-9. 2002-09-01 [PMID: 12486873] (Human)
• Farquhar C, VanCott TC, Mbori-Ngacha DA, Horani L, Bosire RK, Kreiss JK, Richardson BA, John-Stewart GC Salivary secretory leukocyte protease inhibitor is associated with reduced transmission of human immunodeficiency virus type 1 through breast milk. J. Infect. Dis., 2002-09-20;186(8):1173-6. 2002-09-20 [PMID: 12355371] (Human)
• Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study. QJM, 2001-11-01;94(11):599-607. 2001-11-01 [PMID: 11704689] (Human)
• Gompertz S, á—Óã=ÄV¾WÄ, Bayley DL, /@ðtJ‡ ã, Hill SL, Stockley RA Relationship between airway inflammation and the frequency of exacerbations in patients with smoking related COPD. `„ÞRqp±šú°†0~«Â‹9
  ê¢ü JÕÈ$GôÏf –4± beta ~lþ8Wúu“ƒ•îçÐ00©Ù °x°Ï*á|øB´Æ‘?+@“ÞQ}¨»9‡m£Íå8¬vT?[A¥±¶Üùæ¨Ü­nU™-™œŠ!Þ‰ê ÖYí—ÁjyŸÁ}®)¢2A, 2001-01-01;56(1):36-41. 2001-01-01 [PMID: 11120902] (Human)
• Hocini H, Becquart P, Bouhlal H, Adle-Biassette H, Kazatchkine MD, Belec L Secretory leukocyte protease inhibitor inhibits infection of monocytes and lymphocytes with human immunodeficiency virus type 1 but does not interfere with transcytosis of cell-associated virus across tight epithelial barriers. Clin. Diagn. Lab. Immunol., 2000-05-01;7(3):515-8. 2000-05-01 [PMID: 10799472] (Human)

Bioinformatics

• Gene Symbol: SLPI