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SLC3A1 Antibody

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Western Blot: SLC3A1 Antibody [NBP2-93116] - Analysis of extracts of various cell lines, using SLC3A1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: SLC3A1 Antibody [NBP2-93116] - Analysis of extracts of various cell lines, using SLC3A1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug ...read more
Immunocytochemistry/ Immunofluorescence: SLC3A1 Antibody [NBP2-93116] - Analysis of HeLa cells using SLC3A1. Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC3A1 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 416-685 of human SLC3A1 (NP_000332.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC3A1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SLC3A1 Antibody

  • activator of cystine, dibasic and neutral amino acid transport), member 1
  • amino acid transporter 1
  • ATR1SLC3A1 variant C
  • B(0+)-type amino acid transport protein
  • CSNU1
  • D2h
  • D2HSLC3A1 variant D
  • dibasic and neutral amino acid transporters)
  • FLJ34681
  • member 1
  • NBATneutral and basic amino acid transport protein rBAT
  • RBATSLC3A1 variant B
  • SLC3A1 variant E
  • SLC3A1 variant F
  • SLC3A1 variant G
  • solute carrier family 3 (cystine, dibasic and neutral amino acid transporters

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SLC7A9 Antibody [NBP2-93638] - Analysis of extracts of various cell lines, using SLC7A9. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: SLC7A9 Antibody [NBP2-93638] - Analysis of rat kidney using SLC7A9 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: SLC7A9 Antibody [NBP2-93638] - Analysis of human kidney cancer using SLC7A9 at dilution of 1:100. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Angiotensin II/III
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NB100-62346
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
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Western Blot: c-Myb Antibody [NBP1-80306] - WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate
Immunohistochemistry-Paraffin: c-Myb Antibody [NBP1-80306] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: c-Myb Antibody [NBP1-80306] - c-Myb in MOLT-4 cells transfected with siRNA. Image from verified customer review.
(1 Review)
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Immunohistochemistry-Paraffin: CD98 Antibody [NBP2-47515] - Analysis in human kidney and skeletal muscle tissues using NBP2-47515 antibody. Corresponding SLC3A2 RNA-seq data are presented for the same tissues.
Western Blot: CD98 Antibody [NBP2-47515] - Analysis in human cell lines SK-MEL-30 and HEK293 using anti-SLC3A2 antibody. Corresponding SLC3A2 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunocytochemistry/Immunofluorescence: CD98 Antibody [NBP2-47515] - Staining of human cell line U-2 OS shows localization to nucleus, plasma membrane and cytosol.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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NBP2-50465
Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of SLC7A5/LAT1 (BU53) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465AF647 (blue) along with a matched isotype control NB600-986AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 647.
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465 (blue) along with a matched isotype control NB600-986 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
(1 Publication)
Species: Hu
Applications: Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
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NBP1-89200
Western Blot: AGXT Antibody [NBP1-89200] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody (A) NBP1-89200 shows similar protein distribution across tissues to independent antibody NBP2-34198 (B).
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Analysis in human liver and pancreas tissues. Corresponding AGXT RNA-seq data are presented for the same tissues.
(3 Publications)
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Applications: WB, Simple Western, IHC, IHC-P
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Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
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Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry-Paraffin: MMAB Antibody (2G5) [NBP2-03417] Staining of paraffin-embedded Human lymph node tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
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NBP1-83077
Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining in human cerebral cortex and liver tissues. Corresponding ATRX RNA-seq data are presented for the same tissues.
Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP1-83077] - Staining of human cell line A549 shows localization to nucleoplasm and nuclear bodies. Antibody staining is shown in green.
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NB100-308
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
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Prolyl
endopeptidase-like An
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Western Blot: Prolyl endopeptidase-like Antibody [NBP2-93305] - Analysis of extracts of various cell lines, using Prolyl endopeptidase-like at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/Immunofluorescence: Prolyl endopeptidase-like Antibody [NBP2-93305] - Analysis of L929 cells using Prolyl endopeptidase-like. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: Prolyl endopeptidase-like Antibody [NBP2-93305] - Analysis of C6 cells using Prolyl endopeptidase-like. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
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TEF1 Antibody
NBP2-94035
Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-94035] - Analysis of MCF7 cells using TEF1.
Immunohistochemistry-Paraffin: TEF1 Antibody [NBP2-94035] - Mouse heart using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
EAAT3 Antibody
(JU39-69)
NBP2-75476
Western Blot: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: EAAT3 Antibody (JU39-69) [NBP2-75476] - Staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: EAAT3 Antibody (JU39-69) [NBP2-75476] - Analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ASAH1 Antibody
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Western Blot: ASAH1 Antibody [NBP1-89296] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ASAH1 antibody. Corresponding ASAH1 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
(6 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
DIO2 Antibody
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Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody
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Species: Hu, Mu, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
eNOS Antibody
NB300-500
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
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Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, Sh
Applications: WB, IHC, IHC-Fr, IHC-P
LAT Antibody
(LAT.10-17)
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Western Blot: LAT Antibody (LAT.10-17) [NBP2-00289] - 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 ug/mL Anti-Human LAT Purified. Bands were visualized using Anti-Mouse IgG HRP.
Immunohistochemistry-Paraffin: LAT Antibody (LAT.10-17) [NBP2-00289] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Flow (Cell Surface): LAT Antibody (LAT.10-17) [NBP2-00289] - Analysis using the Allophycocyanin conjugate of NBP2-00289. Surface staining of normal human peripheral blood cells with Anti-Human CD3 FITC followed by intracellular staining with Mouse IgG1 kappa Isotype Control APC (left) or Anti-Human LAT APC (right).
Species: Hu
Applications: WB, Flow, IHC, IHC-P, IP, Flow-CS
SERCA2 ATPase
Antibody (2A7-A1)
NB300-581
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
(2 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Po, Am, Ca, Gp, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
SLC3A1 Antibody
NBP2-93116
SLC3A1 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

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Bioinformatics

Gene Symbol SLC3A1