DIO2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Sh, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DIO2 Antibody Summary

Immunogen
Peptide with sequence EVKKHQNQEDRC corresponding to internal region according to NP_054644.1, NP_001007024.1.
Specificity
This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DIO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Frozen 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot
Publications
Read Publications using
NBP1-00178 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Use in Sheep reported in secitific publication PMID: 32403966

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIO2 Antibody

  • 5DII
  • D2
  • deiodinase, iodothyronine, type II
  • DIOII
  • EC 1.97.1
  • EC 1.97.1.10
  • ITDI2
  • SelY
  • TXDI2thyroxine deiodinase, type II
  • Type 2 DI
  • type 2 iodothyronine deiodinase
  • type II iodothyronine deiodinase
  • type-II 5'deiodinase
  • Type-II 5'-deiodinase

Background

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Sh, Rt, Po
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA

Publications for DIO2 Antibody (NBP1-00178)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Sheep
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-00178 Applications Species
Devalraju Kp, Tripathi D, Neela Vsk Et Al. Reduced thyroxine production in young household contacts of tuberculosis patients increases active tuberculosis disease risk JCI insight Jul 8 2021 [PMID: 34236051] (WB) WB
Myers DA, Singleton K, Hyatt K et al. Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb Adipocyte Dec 1 2020 [PMID: 32403966] (WB, Sheep) WB Sheep
Zuo Z, Liu Z, Gao T et al. Prolonged inorganic arsenic exposure via drinking water impairs brown adipose tissue function in mice Sci. Total Environ. Jun 10 2019 [PMID: 30852208] (WB, Mouse) WB Mouse
Kishida T, Ejima A, Yamamoto K et al. Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes. Stem Cell Reports 2015 Sep 09 [PMID: 26365511] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Vose LR, Vinukonda G, Jo S et al. Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage. J Neurosci. 2013 Oct 30 [PMID: 24174657] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Langford D, Baron D, Joy J et al. Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation Psychoneuroendocrinology 2011 Jun [PMID: 21115295] (WB, Human) WB Human
Gumieniak O, Perlstein TS, Williams JS et al. Ala92 Type 2 Deiodinase Allele Increases Risk for the Development of Hypertension. Hypertension 2007 Jan 15 [PMID: 17224473]

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Product General Protocols

View specific protocols for DIO2 Antibody (NBP1-00178): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DIO2
Entrez
Uniprot