SERCA2 ATPase Antibody (2A7-A1)

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

• Reactivity: Hu, Mu, Rt, Po, Am, Ca, Gp, Rb, Sh
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 2A7-A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SERCA2 ATPase Antibody (2A7-A1) Summary

• Immunogen: Purified canine cardiac sarcoplasmic reticulum vesicles.
• Specificity: Detects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase. NB300-581 recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP2A2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 110 kDa protein representing SERCA2 ATPase in rat cardiac tissue.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 21289077). Amphibian reactivity reported in scientific literature (PMID: 12022876). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for SERCA2 ATPase Antibody (2A7-A1)

• ATP2A2
• ATP2B
• ATP2BFLJ20293
• ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
• ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calcium pump 2
• Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
• cardiac Ca2+ ATPase
• DAR
• DD
• EC 3.6.3
• EC 3.6.3.8
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• FLJ38063
• MGC45367
• sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• SERCA2 ATPase
• SERCA2
• SERCA2DKFZp686P0211
• SR Ca(2+)-ATPase 2

Background

ATP dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are comprised of a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SERCA2 ATPase Antibody (NB300-581)(16)

Publications using NB300-581

• Tanton H, Sewastianik T, Seo HS et al. A novel beta-catenin/BCL9 complex inhibitor blocks oncogenic Wnt signaling and disrupts cholesterol homeostasis in colorectal cancer Science advances 2022-04-29 [PMID: 35486727] (ICC/IF)
• Hammad AS, Yu F, Botheju WS Et al. Phosphorylation of STIM1 at ERK/CDK sites is dispensable for cell migration and ER partitioning in mitosis Cell calcium 2021-10-23 [PMID: 34715400] (ICC/IF, Mouse)
• Uchida Y, Yamamoto Y, Sakisaka T Trans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting the Ca2+ pump SERCA2b The Journal of biological chemistry 2021-01-19 [PMID: 33482198]
• Xie D, Geng L, Xiong K et al. Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase Circ. Res. 2020-03-26 [PMID: 32212953] (WB)
• Chung YJ, Luo A, Park KC et al. Iron deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity JCI Insight 2019-02-19 [PMID: 30779710] (WB, Mouse)
• Schroeder MA, Ali MA, Hulikova A et al. Extramitochondrial domain rich in carbonic anhydrase activity improves myocardial energetics. Proc Natl Acad Sci U S A 2013-03-05 [PMID: 23431149] (WB, Rat)
• Klug D, Boule S, Wissocque L et al. Right Ventricular Pacing With Mechanical Dyssynchrony Causes Apoptosis Interruptus and Calcium Mishandling Can J Cardiol 2012-10-10 [PMID: 23062666] (WB, Porcine)
• Garcia-Perez C et al. Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle. J Biol Chem;283(47):32771-80. 2008-11-21 [PMID: 18790739] (IF/IHC)
• Anyatonwu GI et al. Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2. Proc Natl Acad Sci U S A;104(15):6454-9. 2007-04-10 [PMID: 17404231] (IP)
• Prasad AM et al. Phenylephrine hypertrophy, Ca2+-ATPase (SERCA2), and Ca2+ signaling in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol;292(6):C2269-75. 2007-06-01 [PMID: 17287366] (ICC/IF)
• Khan SA et al. Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation-contraction coupling. Proc Natl Acad Sci U S A;101(45):15944-8. 2004-11-09 [PMID: 15486091] (IP)
• Krizaj D, Liu X, Copenhagen DR et al. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004-08-01 [PMID: 15236230] (IF/IHC)
• Spragg DD et al. Regional alterations in protein expression in the dyssynchronous failing heart. Circulation;108(8):929-32. 2003-08-26 [PMID: 12925451] (WB)
• Tupling AR et al. Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs contractile function. J Biol Chem;277(47):44740-6. 2002-11-22 [PMID: 12237298] (ICC/IF)
• Asahi M et al. Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs). J Biol Chem;277(30):26725-8. 2002-07-26 [PMID: 12032137] (IP)
• Movsesian MA et al. Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium. Circulation;90(2):653-7. 1994-08-01 [PMID: 8044934] (WB)

Reviews for SERCA2 ATPase Antibody (NB300-581) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Ike Chinyere IHC-P Rat 12/16/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: cardiac tissue
• Species: Rat
• Lot: UC276383

Comments

• Comments: 1:900 dilution
  Nuclear counterstain

reviewed by: Shinobu Miyazaki ICC Human 08/01/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: hela cell
• Species: Human
• Lot: SE 248905

Bioinformatics

• Gene Symbol: ATP2A2
• Entrez:
  • 403878(Canine)
  • 488(Human)
  • 11938(Mouse)
  • 100038308(Rabbit)
  • 29693(Rat)
• Uniprot:
  • O46674(Canine)