SERCA2 ATPase Antibody (2A7-A1)

Images

 
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. Image from verified ...read more
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Ca, GP, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC/IF
Clone
2A7-A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SERCA2 ATPase Antibody (2A7-A1) Summary

Immunogen
Purified canine cardiac sarcoplasmic reticulum vesicles.
Specificity
Detects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase. NB300-581 recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP2A2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 1:250
  • Immunofluorescence 1:100 - 1:200
Application Notes
WB: Detects an approx. 110 kDa protein representing SERCA2 ATPase in rat cardiac tissue. Used in IHC-Fr.
Reviewed Applications
Read 1 Review rated 5
using
NB300-581 in the following applications:

Publications
Read Publications using
NB300-581 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • IP
    3 publications
  • WB
    4 publications

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 21289077). Amphibian (PMID: 12022876). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SERCA2 ATPase Antibody (2A7-A1)

  • ATP2BFLJ20293
  • ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
  • Calcium pump 2
  • Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform
  • cardiac Ca2+ ATPase
  • DAR
  • DD
  • EC 3.6.3
  • EC 3.6.3.8
  • Endoplasmic reticulum class 1/2 Ca(2+) ATPase
  • FLJ38063
  • MGC45367
  • sarcoplasmic/endoplasmic reticulum calcium ATPase 2
  • SERCA2DKFZp686P0211
  • SR Ca(2+)-ATPase 2

Background

ATP dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are comprised of a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SERCA2 ATPase Antibody (NB300-581)(11)

We have publications tested in 2 confirmed species: Rat, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


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ICC/IF
(2)
IHC
(2)
IP
(3)
WB
(4)
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Rat
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB300-581 Applications Species
Schroeder MA, Ali MA, Hulikova A et al. Extramitochondrial domain rich in carbonic anhydrase activity improves myocardial energetics. Proc Natl Acad Sci U S A 2013 Mar 5 [PMID: 23431149] (WB, Rat) WB Rat
Klug D, Boule S, Wissocque L et al. Right Ventricular Pacing With Mechanical Dyssynchrony Causes Apoptosis Interruptus and Calcium Mishandling Can J Cardiol 2012 Oct 10 [PMID: 23062666] (WB, Porcine) WB Porcine
Garcia-Perez C et al. Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle. J Biol Chem;283(47):32771-80. 2008 Nov 21. [PMID: 18790739] (IHC) IHC
Anyatonwu GI et al. Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2. Proc Natl Acad Sci U S A;104(15):6454-9. 2007 Apr 10. [PMID: 17404231] (IP) IP
Prasad AM et al. Phenylephrine hypertrophy, Ca2+-ATPase (SERCA2), and Ca2+ signaling in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol;292(6):C2269-75. 2007 Jun. [PMID: 17287366] (ICC/IF) ICC/IF
Khan SA et al. Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation-contraction coupling. Proc Natl Acad Sci U S A;101(45):15944-8. 2004 Nov 9. [PMID: 15486091] (IP) IP
Krizaj D et al. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol;475(4):463-80. 2004 Aug 2. [PMID: 15236230] (IHC) IHC
Spragg DD et al. Regional alterations in protein expression in the dyssynchronous failing heart. Circulation;108(8):929-32. 2003 Aug 26. [PMID: 12925451] (WB) WB
Tupling AR et al. Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs contractile function. J Biol Chem;277(47):44740-6. 2002 Nov 22. [PMID: 12237298] (ICC/IF) ICC/IF
Asahi M et al. Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs). J Biol Chem;277(30):26725-8. 2002 Jul 26. [PMID: 12032137] (IP) IP
Show All 11 Publications.

Review for SERCA2 ATPase Antibody (NB300-581) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-581:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry SERCA2 ATPase NB300-581
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reviewed by:
Anonymous
ICC Human 08/01/2018
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Summary

ApplicationImmunocytochemistry
Sample Tested hela cell
SpeciesHuman
LotSE 248905

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SERCA2 ATPase Antibody (NB300-581) (0)

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Blogs on SERCA2 ATPase.

Mending a Broken Heart: New SERCA2 Gene Therapy Fights Heart Disease
While many of the proteins on our antibody database are studied in relation to their expression in diseases; others become therapies in their own right. This is the case with SERCA2 (Sarcoplasmic reticulum Calcium-ATPase 2 pump), which recently hit th...  Read full blog post.

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Recent Reviews

5
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1
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2
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1
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Anonymous
08/01/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol ATP2A2
Entrez
Uniprot