EAAT3 Antibody (4D6.2) - BSA Free

Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747] - Mouse anti-Glutamate Transporter [EAAC1] staining of rat cerebral cortex.

• Reactivity: Rt, Mu, Bv, Rb
• Applications: WB, IHC
• Clone: 4D6.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

EAAT3 Antibody (4D6.2) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_004170.4
• Immunogen: C-terminus peptide (KDKSDTISFTQTSQF).
• Specificity: Glutamate Transporter EAAC1. Based on protein sequence the antibody is expected to react with mouse, bovine and rabbit.
• Predicted Species: Mouse (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC1A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: EAAC1 is approximately 66-70kDa; the protein can form homomultimers during preparation as well, thus often times large 200-220kDa bands can also be seen.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 20mM Phosphate (pH 7.6) and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_004170.4

Alternate Names for EAAT3 Antibody (4D6.2) - BSA Free

• EAAC1
• EAAC1excitatory amino acid carrier 1
• EAAT3
• EAAT3excitatory amino acid transporter 3
• Excitatory amino-acid carrier 1
• Neuronal and epithelial glutamate transporter
• SLC1A1
• Sodium-dependent glutamate/aspartate transporter 3
• solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1
• Solute carrier family 1 member 1

Background

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC1A1
• Uniprot:
  • P43005(Human)