LAT Antibody (LAT.10-17)

Images

 
Western Blot: LAT Antibody (LAT.10-17) [NBP2-00289] - 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 ug/mL Anti-Human LAT Purified. Bands were visualized ...read more
Immunohistochemistry-Paraffin: LAT Antibody (LAT.10-17) [NBP2-00289] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody ...read more
Flow (Cell Surface): LAT Antibody (LAT.10-17) [NBP2-00289] - Analysis using the Allophycocyanin conjugate of NBP2-00289. Surface staining of normal human peripheral blood cells with Anti-Human CD3 FITC followed by ...read more
Immunohistochemistry-Paraffin: LAT Antibody (LAT.10-17) [NBP2-00289] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P, IP
Clone
LAT.10-17
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

LAT Antibody (LAT.10-17) Summary

Immunogen
The immunogen for this antibody is LAT.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)
Application Notes
LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 ug/mL. This LAT.10-17 (10-17) antibody has also been reported for use in immunoprecipitation (IP). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for LAT Antibody (LAT.10-17)

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36

Background

This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLC?, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-00289AF350
Alexa Fluor 405 NBP2-00289AF405
Alexa Fluor 488 NBP2-00289AF488
Alexa Fluor 532 NBP2-00289AF532
Alexa Fluor 594 NBP2-00289AF594
Alexa Fluor 647 NBP2-00289AF647
Alexa Fluor 700 NBP2-00289AF700
Alexa Fluor 750 NBP2-00289AF750
Allophycocyanin NBP2-00289APC
Allophycocyanin/Cy7 NBP2-00289APCCY7
Biotin NBP2-00289B
DyLight 350 NBP2-00289UV
DyLight 405 NBP2-00289V
DyLight 488 NBP2-00289G
DyLight 550 NBP2-00289R
DyLight 594 NBP2-00289DL594
DyLight 650 NBP2-00289C
DyLight 680 NBP2-00289FR
DyLight 755 NBP2-00289IR
FITC NBP2-00289F
HRP NBP2-00289H
Janelia Fluor 549 NBP2-00289JF549
Janelia Fluor 646 NBP2-00289JF646
PE NBP2-00289PE
PE/Atto594 NBP2-00289PEATT594
PE/Cy5.5 NBP2-00289PECY55
PE/Cy7 NBP2-00289PECY7
PerCP NBP2-00289PCP

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Bioinformatics

Gene Symbol LAT