LAT Antibody (LAT.10-17)


Western Blot: LAT Antibody (LAT.10-17) [NBP2-00289] - 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 ug/mL Anti-Human LAT Purified. Bands were visualized more
Immunohistochemistry-Paraffin: LAT Antibody (LAT.10-17) [NBP2-00289] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody more
Flow (Cell Surface): LAT Antibody (LAT.10-17) [NBP2-00289] - Analysis using the Allophycocyanin conjugate of NBP2-00289. Surface staining of normal human peripheral blood cells with Anti-Human CD3 FITC followed by more
Immunohistochemistry-Paraffin: LAT Antibody (LAT.10-17) [NBP2-00289] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P, IP
0.5 mg/ml

Order Details

LAT Antibody (LAT.10-17) Summary

The immunogen for this antibody is LAT.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)
Application Notes
LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 ug/mL. This LAT.10-17 (10-17) antibody has also been reported for use in immunoprecipitation (IP). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified

Alternate Names for LAT Antibody (LAT.10-17)

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36


This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLC?, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: Simple Western, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
Species: Hu
Applications: WB, ICC
Species: Hu, Mu, Rt
Applications: WB, IHC
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for LAT Antibody (NBP2-00289) (0)

There are no publications for LAT Antibody (NBP2-00289).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LAT Antibody (NBP2-00289) (0)

There are no reviews for LAT Antibody (NBP2-00289). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LAT Antibody (NBP2-00289) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-00289AF350
Alexa Fluor 405 NBP2-00289AF405
Alexa Fluor 488 NBP2-00289AF488
Alexa Fluor 532 NBP2-00289AF532
Alexa Fluor 594 NBP2-00289AF594
Alexa Fluor 647 NBP2-00289AF647
Alexa Fluor 700 NBP2-00289AF700
Alexa Fluor 750 NBP2-00289AF750
Allophycocyanin NBP2-00289APC
Allophycocyanin/Cy7 NBP2-00289APCCY7
Biotin NBP2-00289B
DyLight 350 NBP2-00289UV
DyLight 405 NBP2-00289V
DyLight 488 NBP2-00289G
DyLight 550 NBP2-00289R
DyLight 594 NBP2-00289DL594
DyLight 650 NBP2-00289C
DyLight 680 NBP2-00289FR
DyLight 755 NBP2-00289IR
FITC NBP2-00289F
HRP NBP2-00289H
Janelia Fluor 549 NBP2-00289JF549
Janelia Fluor 646 NBP2-00289JF646
PE NBP2-00289PE
PE/Atto594 NBP2-00289PEATT594
PE/Cy5.5 NBP2-00289PECY55
PE/Cy7 NBP2-00289PECY7
PerCP NBP2-00289PCP

Additional LAT Products

Bioinformatics Tool for LAT Antibody (NBP2-00289)

Discover related pathways, diseases and genes to LAT Antibody (NBP2-00289). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LAT Antibody (NBP2-00289)

Discover more about diseases related to LAT Antibody (NBP2-00289).

Pathways for LAT Antibody (NBP2-00289)

View related products by pathway.

PTMs for LAT Antibody (NBP2-00289)

Learn more about PTMs related to LAT Antibody (NBP2-00289).

Blogs on LAT.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our LAT Antibody (LAT.10-17) and receive a gift card or discount.


Gene Symbol LAT