SLC22A3 Antibody (1K0A4)

Western Blot: SLC22A3 Antibody (1K0A4) [NBP3-15897] - Western blot analysis of extracts of various cell lines, using SLC22A3 Rabbit mAb (NBP3-15897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry-Paraffin: SLC22A3 Antibody (1K0A4) [NBP3-15897] - Immunohistochemistry of paraffin-embedded mouse kidney using SLC22A3 Rabbit mAb (NBP3-15897) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SLC22A3 Antibody (1K0A4) [NBP3-15897] - Immunohistochemistry of paraffin-embedded human liver cancer using SLC22A3 Rabbit mAb (NBP3-15897) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 1K0A4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SLC22A3 Antibody (1K0A4) Summary

• Description: Novus Biologicals Rabbit SLC22A3 Antibody (1K0A4) (NBP3-15897) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 301-450 of human SLC22A3 (O75751). GDKALQILRRIAKCNGKYLSSNYSEITVTDEEVSNPSFLDLVRTPQMRKCTLILMFAWFTSAVVYQGLVMRLGIIGGNLYIDFFISGVVELPGALLILLTIERLGRRLPFAASNIVAGVACLVTAFLPEGIAWLRTTVATLGRLGITMAF
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC22A3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SLC22A3 Antibody (1K0A4)

• EMTEMTH
• EMTH
• Extraneuronal monoamine transporter
• OCT3
• OCT3EMT organic cation transporter 3
• Organic cation transporter 3
• SLC22A3
• solute carrier family 22 (extraneuronal monoamine transporter), member 3
• solute carrier family 22 member 3

Background

SLC22A3 - solute carrier family 22 (extraneuronal monoamine transporter), member 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC22A3