SLC22A1 Antibody (2C5)

Images

 
Western Blot: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis using SLC22A1 mAb against HEK293 (1) and SLC22A1(AA: 284-347)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis of Jurkat cells using SLC22A1 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC
Clone
2C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SLC22A1 Antibody (2C5) Summary

Immunogen
Purified recombinant fragment of human SLC22A1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC22A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500 - 1:2000
Application Notes
Use in IHC reported in scientific literature (PMID: 28942964). Use in Electron Microscopy reported in scientific literature (PMID: 28362799). Use in ICC/IF reported in scientific literature (PMID: 26157489).
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-51684 in the following applications:

  • EM
    1 publication
  • 3 publications
  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28942964).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for SLC22A1 Antibody (2C5)

  • hOCT1
  • OCT1
  • oct1_cds
  • OCT1solute carrier family 22 member 1
  • Organic cation transporter 1
  • SLC22A1
  • solute carrier family 22 (organic cation transporter), member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC22A1 Antibody (NBP1-51684)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: EM, ICC/IF, IHC, IHC-P, WB.


Filter By Application
EM
(1)
ICC/IF
(3)
IHC
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-51684 Applications Species
Meyer MJ, Schreier PCF, Basaran M et al. Amino acids in transmembrane helix 1 confer major functional differences between human and mouse orthologs of the polyspecific membrane transporter OCT1 The Journal of biological chemistry Apr 21 2022 [PMID: 35469921] (ICC/IF, Human) ICC/IF Human
Kim HI, Raffler J, Lu W et al. Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport Am. J. Hum. Genet. 2017 Sep 14 [PMID: 28942964] (IHC, Mouse) IHC Mouse
Zhang Y, Boxberger KH, Hagenbuch B. Organic anion transporting polypeptide 1B3 can form homo- and hetero-oligomers PLoS ONE 2017 Jun 23 [PMID: 28644885] (Human) Human
Sekhar GN, Georgian AR, Sanderson L et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS ONE. Mar 31 2017 [PMID: 28362799] (EM, Mouse) EM Mouse
Seitz T, Stalmann R, Dalila N et al. Global genetic analyses reveal strong inter-ethnic variability in the loss of activity of the organic cation transporter OCT1. Genome Med 2015 Jul 09 [PMID: 26157489] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Boxberger KH, Hagenbuch B, Lampe JN. Common Drugs Inhibit Human Organic Cation Transporter 1 (OCT1)-Mediated Neurotransmitter Uptake. Drug Metab. Dispos. 2014 [PMID: 24688079]
Dos Santos Pereira JN et al. The role of membrane transporters in the pharmacokinetics of psychotropic drugs: in vitro studies with special focus on organic cation transporters Thesis. 2014 (IHC-P, Human) IHC-P Human
ROmer S, Meyer MJ, Klein K et al. Effects of a Common Eight Base Pairs Duplication at the Exon 7-Intron 7 Junction on Splicing, Expression, and Function of OCT1 Frontiers in pharmacology May 7 2021 [PMID: 34025422] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC22A1 Antibody (NBP1-51684). (Showing 1 - 1 of 1 FAQs).

  1. We currently have the SLC22A1 antibody (NBP1-51684), and are in the process of developing a quantitation assay with it. However, we are in need of some kind of standard for the OCT1 protein to generate a standard curve for a colorimetric ELISA. We noticed on the Novus website that the product sheet of another SLC22A1 antibody (NBP1-59464) shows the sequence of the peptide-epitope used to generate that antibody, however we cannot find any sequence data for the immunogen used to generate the antibody that we currently have. The only information we have about this immunogen is that it is a purified recombinant fragment of hSLC22A1 expressed in E. coli. So I guess our question is, do you have any information regarding this immunogen (sequence, size, etc) that you could share with us, or can we order some of the immunogen so that we may use this as a standard for quantitating expressed OCT1? Any information would be greatly appreciated!
    • The immunogen used to create this antibody lies between amino acids 284-347. Unfortunately this immunogen is not available for purchase, however.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC22A1
Entrez
Uniprot