SLC22A1 Antibody (2C5)


Western Blot: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis using SLC22A1 mAb against HEK293 (1) and SLC22A1(AA: 284-347)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis of Jurkat cells using SLC22A1 mouse mAb (green) and negative control (purple).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, MS

Order Details

SLC22A1 Antibody (2C5) Summary

Purified recombinant fragment of human SLC22A1 expressed in E. Coli.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Electron Microscopy
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500 - 1:2000
Application Notes
Use in IHC reported in scientific literature (PMID: 28942964). Use in Electron Microscopy reported in scientific literature (PMID: 28362799). Use in ICC/IF reported in scientific literature (PMID: 26157489).
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-51684 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28942964).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide

Alternate Names for SLC22A1 Antibody (2C5)

  • hOCT1
  • OCT1
  • oct1_cds
  • OCT1solute carrier family 22 member 1
  • Organic cation transporter 1
  • SLC22A1
  • solute carrier family 22 (organic cation transporter), member 1


Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. (provided by RefSeq)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: IHC, IHC-P, WB
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Publications for SLC22A1 Antibody (NBP1-51684)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: EM, ICC/IF, IF/IHC, IHC-P, WB.

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Showing Publications 1 - 9 of 9.
Publications using NBP1-51684 Applications Species
Redeker KM, Jensen O, Gebauer L et al. Atypical Substrates of the Organic Cation Transporter 1 Biomolecules 2022-11-09 [PMID: 36359014] (ICC/IF, Human) ICC/IF Human
Meyer MJ, Schreier PCF, Basaran M et al. Amino acids in transmembrane helix 1 confer major functional differences between human and mouse orthologs of the polyspecific membrane transporter OCT1 The Journal of biological chemistry 2022-04-21 [PMID: 35469921] (ICC/IF, Human) ICC/IF Human
Kim HI, Raffler J, Lu W et al. Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport Am. J. Hum. Genet. 2017-09-14 [PMID: 28942964] (IF/IHC, Mouse) IF/IHC Mouse
Zhang Y, Boxberger KH, Hagenbuch B. Organic anion transporting polypeptide 1B3 can form homo- and hetero-oligomers PLoS ONE 2017-06-23 [PMID: 28644885] (Human) Human
Sekhar GN, Georgian AR, Sanderson L et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS ONE. 2017-03-31 [PMID: 28362799] (EM, Mouse) EM Mouse
Seitz T, Stalmann R, Dalila N et al. Global genetic analyses reveal strong inter-ethnic variability in the loss of activity of the organic cation transporter OCT1. Genome Med 2015-07-09 [PMID: 26157489] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Boxberger KH, Hagenbuch B, Lampe JN. Common Drugs Inhibit Human Organic Cation Transporter 1 (OCT1)-Mediated Neurotransmitter Uptake. Drug Metab. Dispos. 2014-01-01 [PMID: 24688079]
Dos Santos Pereira JN et al. The role of membrane transporters in the pharmacokinetics of psychotropic drugs: in vitro studies with special focus on organic cation transporters Thesis. 2014-01-01 (IHC-P, Human) IHC-P Human
ROmer S, Meyer MJ, Klein K et al. Effects of a Common Eight Base Pairs Duplication at the Exon 7-Intron 7 Junction on Splicing, Expression, and Function of OCT1 Frontiers in pharmacology 2021-05-07 [PMID: 34025422] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC22A1 Antibody (NBP1-51684). (Showing 1 - 1 of 1 FAQs).

  1. We currently have the SLC22A1 antibody (NBP1-51684), and are in the process of developing a quantitation assay with it. However, we are in need of some kind of standard for the OCT1 protein to generate a standard curve for a colorimetric ELISA. We noticed on the Novus website that the product sheet of another SLC22A1 antibody (NBP1-59464) shows the sequence of the peptide-epitope used to generate that antibody, however we cannot find any sequence data for the immunogen used to generate the antibody that we currently have. The only information we have about this immunogen is that it is a purified recombinant fragment of hSLC22A1 expressed in E. coli. So I guess our question is, do you have any information regarding this immunogen (sequence, size, etc) that you could share with us, or can we order some of the immunogen so that we may use this as a standard for quantitating expressed OCT1? Any information would be greatly appreciated!
    • The immunogen used to create this antibody lies between amino acids 284-347. Unfortunately this immunogen is not available for purchase, however.

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Gene Symbol SLC22A1