Sirtuin 1/SIRT1 Antibody


Genetic Strategies: Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. more
Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.
Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KO
0.2 mg/ml
Validated by:

Genetic Strategies


Sirtuin 1/SIRT1 Antibody Summary

A genomic peptide made to an internal region of the human SIRT1 protein (within residues 400-550). [Swiss-Prot Q96EB6]
Nucleus, PML body. Note: Recruited to the nuclear bodies via its interaction with PML
Immunogen affinity purified
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  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
Application Notes
This SIRT1 antibody is useful in Immunocytochemistry/Immunfluorescence, Immunhistochemistry paraffin embedded sections and Western blot. In Western blot a band is seen ~105 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Sirtuin 1/SIRT1 Antibody

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1


SIRT1 (mammalian homologue of silent information regulator 2), is a NAD-dependent class III deacetylase which belongs to mammalian sirtuin family and generates enzymatic activity in the presence of NAD+. SIRT1 is primarily localized in the nucleus and deacetylates not only histones but also a number of other transcription factors/cofactors, such as p53, FOXO (forkhead), PPAR- gamma coactivator-1 alpha, and NFkB, thus affecting the critical cellular pathways involved in stress response. SIRT1 plays an important role in the energy metabolism by interacting with PPAR-gamma and inhibiting its transcriptional activity towards genes mediating fat storage through docking with its cofactors. SIRT1 also plays an essential function in mediating cellular survival by deacetylating substrates such as FOXO, p53, DNA repair factor Ku70 and NFkB. SIRT1 has been shown to get upregulated in response to stress-induced cell death, especially upon oxidative stress and calorie restriction, and SIRT1 knockout mice fail to extend lifespan induced by calorie restriction. Moreover, expression of SIRT1 gets increased to promote neuronal survival in Alzheimer's disease model as well as in primary neurons subjected to neurotoxic insult.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49540AF405
Alexa Fluor 488 Labeled NBP1-49540AF488
Alexa Fluor 647 Labeled NBP1-49540AF647
Alexa Fluor 700 Labeled NBP1-49540AF700
Biotin Labeled NBP1-49540B
DyLight 488 Labeled NBP1-49540G
DyLight 550 Labeled NBP1-49540R
DyLight 650 Labeled NBP1-49540C
HRP Labeled NBP1-49540H

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Gene Symbol SIRT1