Sirtuin 1/SIRT1 Antibody

Images

 
Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.
Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Sirtuin 1/SIRT1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human SIRT1 protein (within residues 400-550). [Swiss-Prot Q96EB6]
Localization
Nucleus, PML body. Note: Recruited to the nuclear bodies via its interaction with PML
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Application Notes
This SIRT1 antibody is useful in Immunocytochemistry/Immunfluorescence, Immunhistochemistry paraffin embedded sections and Western blot. In Western blot a band is seen ~105 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Sirtuin 1/SIRT1 Antibody

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Background

SIRT1 (mammalian homologue of silent information regulator 2), is a NAD-dependent class III deacetylase which belongs to mammalian sirtuin family and generates enzymatic activity in the presence of NAD+. SIRT1 is primarily localized in the nucleus and deacetylates not only histones but also a number of other transcription factors/cofactors, such as p53, FOXO (forkhead), PPAR- gamma coactivator-1 alpha, and NFkB, thus affecting the critical cellular pathways involved in stress response. SIRT1 plays an important role in the energy metabolism by interacting with PPAR-gamma and inhibiting its transcriptional activity towards genes mediating fat storage through docking with its cofactors. SIRT1 also plays an essential function in mediating cellular survival by deacetylating substrates such as FOXO, p53, DNA repair factor Ku70 and NFkB. SIRT1 has been shown to get upregulated in response to stress-induced cell death, especially upon oxidative stress and calorie restriction, and SIRT1 knockout mice fail to extend lifespan induced by calorie restriction. Moreover, expression of SIRT1 gets increased to promote neuronal survival in Alzheimer's disease model as well as in primary neurons subjected to neurotoxic insult.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sirtuin 1/SIRT1 Antibody (NBP1-49540) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49540AF405
Alexa Fluor 488 Labeled NBP1-49540AF488
Alexa Fluor 647 Labeled NBP1-49540AF647
Alexa Fluor 700 Labeled NBP1-49540AF700
Biotin Labeled NBP1-49540B
DyLight 488 Labeled NBP1-49540G
DyLight 550 Labeled NBP1-49540R
DyLight 650 Labeled NBP1-49540C
HRP Labeled NBP1-49540H

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Bioinformatics

Gene Symbol SIRT1
Entrez
Uniprot