Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-78098] - Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in buffer over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (shown as green) DyLight488 Donkey anti-Mouse IgG (shown as blue), and DyLight649 Donkey anti-Goat IgG (shown as red) at 1:10000 (in buffer 30 min RT).

Product Discontinued

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free Summary

• Description: This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins attached to a solid phase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Human Serpin A1/alpha 1-Antitrypsin (Uniprot: P01009)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:600000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:500 -1:2500
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested by LEISA, SDS-PAGE, and western blot. This product is assayed against 1.0 ug of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Alpha 1 Antitrypsin, also known as SERPINA1, is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A1/alpha 1-Antitrypsin Antibody (NBP1-78098)(1)

Publication using NBP1-78098

• Holzer M, Ljubojevic-Holzer S, Souza Junior D et al. HDL isolated by immunoaffinity, ultracentrifugation, or precipitation is compositionally and functionally distinct. Journal of Lipid Research 2022-10-01 [PMID: 36511335]

Bioinformatics

• Gene Symbol: SERPINA1
• Entrez:
  • 5265(Human)
• Uniprot:
  • P01009()
  • P01009()
  • P01009()
  • P01009()
  • P01009()