alpha 2-Macroglobulin Antibody [Unconjugated]

Images

 
Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human alpha ...read more
Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated ...read more
alpha 2‑Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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alpha 2-Macroglobulin Antibody [Unconjugated] Summary

Immunogen
Human plasma-derived alpha 2-Macroglobulin
Specificity
Detects human alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha 2-Macroglobulin-like 1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
A2M
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 1 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1938 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody [Unconjugated]

  • A2M
  • alpha 2Macroglobulin
  • alpha 2-Macroglobulin
  • alpha-2-M
  • alpha-2-macroglobulin
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
  • CPAMD5
  • CPAMD5DKFZp779B086
  • FWP007
  • S863-7

Background

Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

  1. Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
  2. Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha 2-Macroglobulin Antibody (AF1938)(2)

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Bioinformatics

Gene Symbol A2M