alpha 2-Macroglobulin Antibody [Unconjugated]

HAF017). A specific band was detected for a₂-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for a₂-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a₂-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." > HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." title="Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for a₂-Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." />

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for a₂-Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="a₂-Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

a₂-Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human a₂-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

1938-PI) was serially diluted and captured by Mouse Anti-Human ?₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ?₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 1938-PI) was serially diluted and captured by Mouse Anti-Human ?₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ?₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Human ?₂‑Macroglobulin (Catalog # 1938-PI) was serially diluted and captured by Mouse Anti-Human ?₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ?₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Human ?₂‑Macroglobulin (Catalog # <a class=

1938-PI) was serially diluted and captured by Mouse Anti-Human alpha ₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha ₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 1938-PI) was serially diluted and captured by Mouse Anti-Human alpha ₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha ₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Human alpha ₂‑Macroglobulin (Catalog # 1938-PI) was serially diluted and captured by Mouse Anti-Human alpha ₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha ₂‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Human alpha ₂‑Macroglobulin (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, Simple Western, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 2-Macroglobulin Antibody [Unconjugated] Summary

• Immunogen: Human plasma-derived alpha ₂-Macroglobulin
• Specificity: Detects human alpha ₂-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha ₂-Macroglobulin-like 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: A2M
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody [Unconjugated]

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

Human alpha ₂-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

1. Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
2. Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2-Macroglobulin Antibody (AF1938)(4)

Publications using AF1938

• S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8 The Journal of Biological Chemistry, 2021-10-21;0(0):101323. 2021-10-21 [PMID: 34687701] (Western Blot, Human)
• Y Suzuki, K Hashimoto, K Hoshi, H Ito, Y Kariya, K Miyazaki, M Sato, Y Kawasaki, M Yoshida, T Honda, Y Hashimoto, M Hosoya Ratio of Alpha 2-Macroglobulin Levels in Cerebrospinal Fluid and Serum: An Expression of Neuroinflammation in Acute Disseminated Encephalomyelitis Pediatr. Neurol., 2019-05-11;0(0):. 2019-05-11 [PMID: 31248670] (IHC, Human)
• Dalli , Jesmond, Norling , Lucy V, Montero-Melendez , Trinidad, Federici Canova , Donata, Lashin , Hazem, Pavlov , Anton M, Sukhorukov , Gleb B, Hinds , Charles, Perretti , Mauro Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis. EMBO Mol Med, 2013-12-15;6(1):27-42. 2013-12-15 [PMID: 24357647] (ICC, Human)
• Kim JC, Kim JY, Yeom SR, Jeong BY, Hwang HZ, Park KJ, Lee SW 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 2008-09-01;8(17):3632-44. 2008-09-01 [PMID: 18686296] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: A2M