Serpin A3N: Novus Biologicals

AF4709

Serpin A3N Antibody [Unconjugated]

(36 Publications)

100 μg

AF4709

Serpin A3N + labelled oligodendrocytes can be seen in the Corpus callosum of cuprizone-intoxicated mice. Primary antibody: Serpin A3N 1:1000, 18h incubation at 4°CSecondary antibody: rabbit anti goat, 1:200, 21°C, 1h incubation. Visualization via DAB. Image from a verified customer review.

HS diet increased the serpina3n expression in the hippocampus of APP/PS1 mice. (A) GSEA showed that the HS diet intervention changed the status of axon guidance, neuroactive ligand–receptor interaction, oxidoreductase activity, and blood vessel diameter. (B) A radar map of differential gene expression levels. Serpina3n is most significantly changed, and its mRNA expression is the highest among the serpin family. (C) Protein level of serpina3n was assessed using Western blotting; GAPDH was used as a control. n = 4 biologically independent animals. (D) mRNA levels of the serpin family in HS- and ND-treated APP/PS1 mice were compared; serpina3n is the most remarkably changed mRNA. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/21/11731), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4709U-100UG
Alexa Fluor 405	AF4709V-100UG
Alexa Fluor 488	AF4709G-100UG
Alexa Fluor 532	AF4709X-100UG
Alexa Fluor 594	AF4709T-100UG
Alexa Fluor 647	AF4709R-100UG
Alexa Fluor 700	AF4709N-100UG
Alexa Fluor 750	AF4709S-100UG
Alexa Fluor Plus 405	AF4709AFP405-100UG
Alexa Fluor Plus 488	AF4709AFP488-100UG
Alexa Fluor Plus 555	AF4709AFP555-100UG
Alexa Fluor Plus 594	AF4709AFP594-100UG
Alexa Fluor Plus 647	AF4709AFP647-100UG
Alexa Fluor Plus 680	AF4709AFP680-100UG
Allophycocyanin	AF4709A
Biotin	AF4709B
CoraFluor 1	AF4709CL1
DyLight 350	AF4709D
DyLight 405	AF4709E
DyLight 488	AF4709K
DyLight 550	AF4709L
DyLight 594	AF4709M
DyLight 650	AF4709W
DyLight 680	AF4709Y
DyLight 755	AF4709Z
FITC	AF4709F
HRP	AF4709H
Janelia Fluor 525	AF4709JF525
Janelia Fluor 549	AF4709I
Janelia Fluor 646	AF4709J
Janelia Fluor 669	AF4709JF669
PE	AF4709P
PerCP	AF4709C
mFluor Violet 450 SE	AF4709MFV450
mFluor Violet 500 SE	AF4709MFV500
mFluor Violet 610 SE	AF4709MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4709-SP

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MAB1268

Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]

(1 Review)

(13 Publications)

100 μg

MAB1268

Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Serpin A1/ alpha 1‑Antitrypsin knockout HepG2 cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated secondary antibody. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 46.7 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. Primary antibody dilution used: 1 ug/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

$<b>Genetic Strategies Validation. </b>HepG2 human hepatocellular carcinoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 µg of Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) pre-coupled to Dynabeads Protein G. Immunoprecipitated Serpin A1/ alpha 1‑Antitrypsin was detected in Western Blot with a rabbit anti-Serpin A1 used at 1/5000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Serpin A1/ a1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Serpin A1/ alpha 1‑Antitrypsin was detected in immersion fixed paraffin-embedded sections of Human Prostate using Mouse Anti-Human Serpin A1/ alpha 1‑Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1268U-100UG
Alexa Fluor 405	FAB1268V-100UG
Alexa Fluor 488	FAB1268G-100UG
Alexa Fluor 532	FAB1268X-100UG
Alexa Fluor 594	FAB1268T-100UG
Alexa Fluor 647	FAB1268R-100UG
Alexa Fluor 700	FAB1268N-100UG
Alexa Fluor 750	FAB1268S-100UG
Alexa Fluor Plus 405	FAB1268AFP405-100UG
Alexa Fluor Plus 488	FAB1268AFP488-100UG
Alexa Fluor Plus 555	FAB1268AFP555-100UG
Alexa Fluor Plus 594	FAB1268AFP594-100UG
Alexa Fluor Plus 647	FAB1268AFP647-100UG
Alexa Fluor Plus 680	FAB1268AFP680-100UG
Allophycocyanin	FAB1268A
Biotin	FAB1268B
CoraFluor 1	FAB1268CL1
DyLight 350	FAB1268D
DyLight 405	FAB1268E
DyLight 488	FAB1268K
DyLight 550	FAB1268L
DyLight 594	FAB1268M
DyLight 650	FAB1268W
DyLight 680	FAB1268Y
DyLight 755	FAB1268Z
FITC	FAB1268F
HRP	FAB1268H
Janelia Fluor 525	FAB1268JF525
Janelia Fluor 549	FAB1268I
Janelia Fluor 646	FAB1268J
Janelia Fluor 669	FAB1268JF669
PE	FAB1268P
PerCP	FAB1268C
mFluor Violet 450 SE	FAB1268MFV450
mFluor Violet 500 SE	FAB1268MFV500
mFluor Violet 610 SE	FAB1268MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1268-SP
Carrier Free	MAB1268-500

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DY1268

Human Serpin A1 DuoSet ELISA, 15 Plate

(19 Publications)

1 Kit (for 15 Plates)

DY1268

N/A Serpin A1/alpha 1-Antitrypsin [Biotin]

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-90309

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

(8 Publications)

0.1 ml

NBP1-90309

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis in human kidney and cerebral cortex tissues using NBP1-90309 antibody. Corresponding SERPINA1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human kidney shows moderate positivity in plasma.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human placenta shows strong positivity in plasma in blood vessels.

Staining of human testis shows moderate positivity in plasma.

Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-NBP1-90309-img0031.jpg

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Representative expression status for ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, serpine1 & IL8 levels in tumor tissue.Insert Representative expression status for ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, serpine1 & IL-8 levels in benign tissue. All images were captured at 400× magnification. Image collected & cropped by CiteAb from the following publication (//diagnosticpathology.biomedcentral.com/articles/10.1186/s13000-014-0200-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - SerpinA1 was regulated by SnailA. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), & Snail & serpinA1 protein levels were evaluated by western blot analysis. B. DLD-1 & SW480 cells were transfected with pcDNA-serpinA1 (serpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), & western blot analysis was performed for detection of Snail & SerpinA1 expression. C. DLD-1 & SW480 cells were transfected with pcDNA-Snail (Snail) or control vector pcDNA (vector), & ChIP assays were performed. The presence of the serpinA1 promoter (−516/−4) was verified in immunoprecipitates with either mouse IgG or anti-Snail antibodies, & assay inputs were analyzed using real-time PCR. The samples were loaded on agarose gels. D. Data show promoter enrichment in the anti-Snail immunoprecipitate relative to IgG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-NBP1-90309-img0030.jpg

<b>Independent Antibodies Validation. </b>Analysis using antibody NBP1-90309 (A) shows similar pattern to independent antibody NBP1-90308 (B).

Staining of human cell line Hep G2 shows localization to vesicles.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90309-25ul

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NBP2-52557

Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) - BSA Free

(1 Publication)

0.1 mg

NBP2-52557

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of paraffin-embedded bladder cancer tissues using SERPINA1 mouse mAb with DAB staining.

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - An intracellular stain was performed on A549 cells with Serpin AI/alpha I-Antitrypsin antibody (5DID2) NBP2-52557APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis using SERPINA1 mAb against human SERPINA1 (AA: 23-237) recombinant protein. (Expected MW is 50.3 kDa)

Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of GC-7901 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of HepG2 cells using SERPINA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of paraffin-embedded ovarian cancer tissues using SERPINA1 mouse mAb with DAB staining.

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Analysis of Hela cells using SERPINA1 mouse mAb (green) and negative control (red).

ELISA: Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) [NBP2-52557] - Black line: Control Antigen (100 ng); Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52557-0.025mg

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AF1268

Serpin A1/alpha 1-Antitrypsin Antibody [Unconjugated]

(3 Publications)

100 μg

AF1268

HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in Serpin A1/a1-Antitrypsin knockout (KO) HepG2 cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 9 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-55 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Serpin A1/a1-Antitrypsin HepG2 knockout cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-55 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1268U-100UG
Alexa Fluor 405	AF1268V-100UG
Alexa Fluor 488	AF1268G-100UG
Alexa Fluor 532	AF1268X-100UG
Alexa Fluor 594	AF1268T-100UG
Alexa Fluor 647	AF1268R-100UG
Alexa Fluor 700	AF1268N-100UG
Alexa Fluor 750	AF1268S-100UG
Alexa Fluor Plus 405	AF1268AFP405-100UG
Alexa Fluor Plus 488	AF1268AFP488-100UG
Alexa Fluor Plus 555	AF1268AFP555-100UG
Alexa Fluor Plus 594	AF1268AFP594-100UG
Alexa Fluor Plus 647	AF1268AFP647-100UG
Alexa Fluor Plus 680	AF1268AFP680-100UG
Allophycocyanin	AF1268A
Biotin	AF1268B
CoraFluor 1	AF1268CL1
DyLight 350	AF1268D
DyLight 405	AF1268E
DyLight 488	AF1268K
DyLight 550	AF1268L
DyLight 594	AF1268M
DyLight 650	AF1268W
DyLight 680	AF1268Y
DyLight 755	AF1268Z
FITC	AF1268F
HRP	AF1268H
Janelia Fluor 525	AF1268JF525
Janelia Fluor 549	AF1268I
Janelia Fluor 646	AF1268J
Janelia Fluor 669	AF1268JF669
PE	AF1268P
PerCP	AF1268C
mFluor Violet 450 SE	AF1268MFV450
mFluor Violet 500 SE	AF1268MFV500
mFluor Violet 610 SE	AF1268MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1268-SP

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KA0459

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

(4 Publications)

1 Kit

KA0459

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Standard Curve:

0.391 to 25 ng/mL

Sensitivity:

0.31 ng/mL

Sample Type:

Cell Culture Supernatant, Cell Lysate, CSF, Milk, Plasma, Saliva, Serum, Urine

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NBP1-90308

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-90308

<b>Orthogonal Strategies Validation. </b>Analysis in human kidney and cerebral cortex tissues using NBP1-90308 antibody. Corresponding SERPINA1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human kidney shows moderate positivity in plasma.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human placenta shows strong positivity in plasma in blood vessels.

Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Staining of human testis shows moderate positivity in plasma.

<b>Independent Antibodies Validation. </b>Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90308] - Analysis using Anti-SERPINA1 antibody NBP1-90308 (A) shows similar pattern to independent antibody NBP1-90309 (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90308-25ul

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NB600-602

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

(5 Publications)

0.1 mg

NB600-602

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
HRP	NB120-7635-0.1mg

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NBP3-12165

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

96 wells

NBP3-12165

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.24 - 200 ng/mL

Sample Type:

Serum, Plasma, Colostrum, Milk

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AF2979

Serpin A1c/alpha 1-Antitrypsin Antibody [Unconjugated]

(2 Publications)

100 μg

AF2979

HAF017). A specific band was detected for Serpin A1c/a1-Antitrypsin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin A1c/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Serpin A1c/a1-Antitrypsin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin A1c/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin A1c/a1-Antitrypsin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows mouse serum and lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1c/a1-Antitrypsin at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Serpin A1c/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Serpina1a mutants imitate intracellular distribution of human Z-A1AT. Confocal laser immunofluorescence analysis of COS-7 cells expressing wild type (wt; top row), E337K, H329D or H329D_E337K double mutant Serpina1a (second to fourth row), compared to cells overexpressing normal human M-A1AT or E342K Z-A1AT (fifth and sixth row). Mouse Serpina1a and human A1AT were stained with Alexa Fluor 568 secondary antibody (green) and exposed to 0.6% laser light transmission (LLTM). Mutant-expressing cells were additionally exposed to 0.1% LLTM, as the very strong signal resulted in over-saturation at 0.6%. ER-marker Grp78/BiP was stained with Alexa Fluor 647 secondary antibody (red) and cell nucleus was stained using DAPI (blue). Scale bar: 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31097772), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2979U-100UG
Alexa Fluor 405	AF2979V-100UG
Alexa Fluor 488	AF2979G-100UG
Alexa Fluor 532	AF2979X-100UG
Alexa Fluor 594	AF2979T-100UG
Alexa Fluor 647	AF2979R-100UG
Alexa Fluor 700	AF2979N-100UG
Alexa Fluor 750	AF2979S-100UG
Alexa Fluor Plus 405	AF2979AFP405-100UG
Alexa Fluor Plus 488	AF2979AFP488-100UG
Alexa Fluor Plus 555	AF2979AFP555-100UG
Alexa Fluor Plus 594	AF2979AFP594-100UG
Alexa Fluor Plus 647	AF2979AFP647-100UG
Alexa Fluor Plus 680	AF2979AFP680-100UG
Allophycocyanin	AF2979A
Biotin	AF2979B
CoraFluor 1	AF2979CL1
DyLight 350	AF2979D
DyLight 405	AF2979E
DyLight 488	AF2979K
DyLight 550	AF2979L
DyLight 594	AF2979M
DyLight 650	AF2979W
DyLight 680	AF2979Y
DyLight 755	AF2979Z
FITC	AF2979F
HRP	AF2979H
Janelia Fluor 525	AF2979JF525
Janelia Fluor 549	AF2979I
Janelia Fluor 646	AF2979J
Janelia Fluor 669	AF2979JF669
PE	AF2979P
PerCP	AF2979C
mFluor Violet 450 SE	AF2979MFV450
mFluor Violet 500 SE	AF2979MFV500
mFluor Violet 610 SE	AF2979MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2979-SP

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1268-PI

Recombinant Human Serpin A1/alpha-1-Antitrypsin Protein, CF

(5 Publications)

10 μg

1268-PI

ES002)." class="big_lightbox">

ES002)." alt="Recombinant Human Serpin A1/alpha-1-Antitrypsin (Catalog # 1268-PI) is measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

SERPINA1 Products By Gene Name

Serpin A3N Antibody [Unconjugated]

Serpin A1/alpha 1-Antitrypsin Antibody (202808) [Unconjugated]

Human Serpin A1 DuoSet ELISA, 15 Plate

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Serpin A1/alpha 1-Antitrypsin Antibody (5D1D2) - BSA Free

Serpin A1/alpha 1-Antitrypsin Antibody [Unconjugated]

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

Serpin A1c/alpha 1-Antitrypsin Antibody [Unconjugated]

Recombinant Human Serpin A1/alpha-1-Antitrypsin Protein, CF