Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free

Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis using SERPIN A3 mouse mAb against A549 cell lysate.

Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis of paraffin-embedded bladder cancer tissues using SERPINA3 mouse mAb with DAB staining.

Flow Cytometry: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis of A549 cells using SERPINA3 mouse mAb (green) and negative control (red).

Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis using SERPINA3 mAb against HEK293 (1) and SERPINA3 (AA: 279-432)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis using SERPINA3 mAb against human SERPINA3 (AA: 279-432) recombinant protein. (Expected MW is 42.3 kDa)

Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Analysis of paraffin-embedded endometrial cancer tissues using SERPINA3 mouse mAb with DAB staining.

ELISA: Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) [NBP2-52559] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
• Clone: 5G3C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human SERPINA3 (AA: 279-432) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 47.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free

• AACTMGC88254
• ACTGIG25
• alpha 1-Antichymotrypsin
• alpha-1-antichymotrypsin
• antitrypsin), member 3
• Cell growth-inhibiting gene 24/25 protein
• growth-inhibiting protein 24
• growth-inhibiting protein 25
• member 3
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 3
• Serpin A3
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Alpha 1 Antichymotrypsin is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variation in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A3/alpha 1-Antichymotrypsin Antibody (NBP2-52559)(1)

Publication using NBP2-52559

• Neidleman JA, Chen JC, Kohgadai N et al. Mucosal stromal fibroblasts markedly enhance HIV infection of CD4+ T cells. PLoS Pathog. 2017-02-01 [PMID: 28207890] (ELISA, Human)

Bioinformatics

• Gene Symbol: SERPINA3