Serpin A1/alpha 1-Antitrypsin Antibody [Unconjugated]

Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Serpin A1/a1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in Serpin A1/a1-Antitrypsin knockout (KO) HepG2 cell line using Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 9 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Serpin A1/a1-Antitrypsin HepG2 knockout cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Serpin A1/a1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-55 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, IP, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A1/alpha 1-Antitrypsin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha 1‑Antitrypsin
  Glu25-Lys418
  Accession # P01009
• Specificity: Detects human Serpin A1/ alpha 1‑Antitrypsin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A1/alpha 1-Antitrypsin Antibody [Unconjugated]

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency (3).

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
2. Barbour, K.W. et al. (2002) Genomics 80:515.
3. Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A1/alpha 1-Antitrypsin Antibody (AF1268)(3)

Publications using AF1268

• David Nardo, Caleb M. Akers, Nicholas E. Cheung, Cierra M. Isom, Jason T. Spaude, Daniel W. Pack et al. Cyanuric chloride as the basis for compositionally diverse lipids† RSC Advances 7/13/2021 [PMID: 34354826]
• F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 2020-12-01;33(9):108453. 2020-12-01 [PMID: 33264615] (ICC, IHC, Human)
• Grek C, Townsend D, Uys J, Manevich Y, Coker W, Pazoles C, Tew K S-glutathionylated serine proteinase inhibitors as plasma biomarkers in assessing response to redox-modulating drugs. Cancer Res, 2012-03-08;72(9):2383-93. 2012-03-08 [PMID: 22406622] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPINA1
• Uniprot:
  • P01009()