SALL4 Antibody - BSA Free

Western Blot: SALL4 Antibody [NBP1-85206] - Analysis NBP1-85206 (A) shows similar pattern to independent antibody NBP1-84247 (B).

Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP1-85206] - Staining of human cell line CACO-2 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human pancreas shows no positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human ovary shows strong nuclear positivity in follicle cells.

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human colon, lymph node, ovary and testis using Anti-SALL4 antibody NBP1-85206 (A) shows similar protein distribution across tissues to independent antibody NBP1-84247 (B).

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human colon shows no positivity in glandular cells.

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-85206] - Staining of human lymph node shows no positivity in germinal center cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SALL4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PSVNVDPVVWNQYTSMLNGGLAVKTNEISVIQSGGVPTLPVSLGATSVVNNATVSKMDGSQSGISADVEKPSATDGVPKHQFPHFLEENKIA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SALL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SALL4 Protein (NBP1-85206PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SALL4 Antibody - BSA Free

• dJ1112F19.1
• DRRS
• HSAL4
• MGC133050
• SALL4
• sal-like 4 (Drosophila)
• sal-like protein 4
• Zinc finger protein 797
• Zinc finger protein SALL4
• ZNF797
• ZNF797sal (Drosophila)-like 4

Background

Sall4 is encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SALL4