Glypican 3 Antibody (1G12)

Western Blot: Glypican 3 Antibody (1G12) [NBP2-44484] - Western Blot Analysis of HePG2 cell lysate using Glypican 3 Antibody (1G12)

Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (1G12) [NBP2-44484] - ICC/IF analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)

Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) [NBP2-44484] - Analysis of human liver tissue using Glypican 3 antibody. Fixed paraffin-embedded sections were deparaffinized. antigen retrieval was done enzymatically. Primary antibody dilution was 1:100. Image from verified customer review.

Flow Cytometry: Glypican 3 Antibody (1G12) [NBP2-44484] - Flow Cytometric Analysis of MeOH-fixed HepG2 cells using Glypican 3 Antibody (1G12) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) [NBP2-44484] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (1G12)

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, ICC/IF
• Clone: 1G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Glypican 3 Antibody (1G12) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47760)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A recombinant fragment containing amino acids 511-580 of human Glypican 3 (Uniprot: P51654)
• Localization: Cytoplasmic
• Marker: Hepatocellular Carcinoma Marker
• Specificity: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Glypican 3 Antibody (1G12)

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 3 Antibody (NBP2-44484)(1)

Publication using NBP2-44484

• Makkouk A, Yang XC, Barca T et al. Off-the-shelf V delta 1 gamma delta T cells engineered with glypican-3 (GPC-3)-specific chimeric antigen receptor (CAR) and soluble IL-15 display robust antitumor efficacy against hepatocellular carcinoma Journal for immunotherapy of cancer 2021-12-01 [PMID: 34916256] (FLOW)

Review for Glypican 3 Antibody (NBP2-44484) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/02/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver tissue
• Species: Human
• Lot: 1G12

Comments

• Comments: fixed paraffin-embedded sections were deparaffinized. antigen retrieval was done enzymatically. primary antibody dilution was 1:100.

Bioinformatics

• Gene Symbol: GPC3
• Uniprot:
  • P51654()
  • P51654()
  • P51654()
  • P51654()