| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21209-25ul | |||
| Azide and BSA Free | NBP3-44348 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21208-25ul | |||
| Azide and BSA Free | NBP3-44347 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85206-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84247-25ul | |||
HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6374-SP | |||
![Western Blot: SALL4 Antibody (6E3) [H00057167-M03] - Analysis of SALL4 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SALL4-Antibody-6E3-Western-Blot-H00057167-M03-img0011.jpg "Western Blot: SALL4 Antibody (6E3) [H00057167-M03] - Analysis of SALL4 expression in Hela S3 NE (Cat # L013V3).")
![Immunohistochemistry-Paraffin: SALL4 Antibody (6E3) [H00057167-M03] - Analysis of monoclonal antibody to SALL4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.2 ug/ml](http://images.novusbio.com/fullsize/SALL4-Antibody-6E3-Immunohistochemistry-Paraffin-H00057167-M03-img0010.jpg)
![Western Blot: SALL4 Antibody (6E3) [H00057167-M03] - Analysis of SALL4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/SALL4-Antibody-6E3-Western-Blot-H00057167-M03-img0012.jpg)
![ELISA: SALL4 Antibody (6E3) [H00057167-M03] - Detection limit for recombinant GST tagged SALL4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SALL4-Antibody-6E3-ELISA-H00057167-M03-img0005.jpg)
![Western Blot: SALL4 Antibody [NBP1-85206] - Analysis NBP1-85206 (A) shows similar pattern to independent antibody NBP1-84247 (B).](http://images.novusbio.com/fullsize/SALL4-Antibody-Western-Blot-NBP1-85206-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SALL4 Antibody [NBP1-85206] - Analysis NBP1-85206 (A) shows similar pattern to independent antibody NBP1-84247 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP1-85206] - Staining of human cell line CACO-2 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85206-img0004.jpg)
![Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunohistochemistry-Paraffin-NBP1-84247-img0014.jpg "Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human testis shows weak to moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunohistochemistry-Paraffin-NBP1-84247-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human colon, lymph node, ovary and testis using Anti-SALL4 antibody NBP1-84247 (A) shows similar protein distribution across tissues to independent antibody NBP1-85206 (B).](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunohistochemistry-Paraffin-NBP1-84247-img0011.jpg)
![Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunohistochemistry-Paraffin-NBP1-84247-img0012.jpg)
![Immunohistochemistry-Paraffin: SALL4 Antibody [NBP1-84247] - Staining of human ovary shows weak nuclear positivity in follicle cells.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunohistochemistry-Paraffin-NBP1-84247-img0013.jpg)
![Western Blot: SALL4 Antibody [NBP1-32969] - SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SALL4-Antibody-Western-Blot-NBP1-32969-img0009.jpg "Western Blot: SALL4 Antibody [NBP1-32969] - SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP1-32969] - Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SALL4 Antibody at 1:400 dilution.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32969-img0007.jpg)
![Immunohistochemistry-Paraffin: Sall4 Antibody [NBP1-32969] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Sall4-Antibody-Immunohistochemistry-Paraffin-NBP1-32969-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Sall4 Antibody [NBP1-32969] - Paraformaldehyde-fixed Human ESC, using SALL4 antibody at 1:400 dilution.](http://images.novusbio.com/fullsize/Sall4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32969-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP1-32969] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SALL4 Antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SALL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32969-img0006.jpg)
![Immunohistochemistry: SALL4 Antibody (A9G9-R) [NBP3-32926] - Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (NBP3-32926) at 1/4,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32926) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32926_mouse-sall4-mab-a9g9-r-298202414262683.jpg "Immunohistochemistry: SALL4 Antibody (A9G9-R) [NBP3-32926] - Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (NBP3-32926) at 1/4,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32926) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: SALL4 Antibody (A9G9-R) [NBP3-32926] - Immunofluorescence analysis of paraffin-embedded human seminoma tissue labeling SALL4 with Mouse anti-SALL4 antibody (NBP3-32926) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32926, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32926_mouse-sall4-mab-a9g9-r-298202414304169.jpg)
![Immunocytochemistry/ Immunofluorescence: SALL4 Antibody (A9G9-R) [NBP3-32926] - Immunocytochemistry analysis of NCCIT cells labeling SALL4 with Mouse anti-SALL4 antibody (NBP3-32926) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-SALL4 antibody (NBP3-32926) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32926_mouse-sall4-mab-a9g9-r-29202410234234.jpg)
![Flow Cytometry: SALL4 Antibody (A9G9-R) [NBP3-32926] - Flow cytometric analysis of NCCIT cells labeling SALL4.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32926, 1ug/ml) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32926_mouse-sall4-mab-a9g9-r-29202416372156.jpg)
![Western blot analysis of NCCIT cell lysates with NBP3-32926 at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 112 kDa Observed band size: 140/110/75 kDa Exposure time: 1 minute 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32926 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32926_-western-blot-639204590074974507.jpg)
![Immunohistochemistry: SALL4 Antibody (A7A7-R) [NBP3-32927] - Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (NBP3-32927) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32927) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32927_mouse-sall4-mab-a7a7-r-298202414333634.jpg "Immunohistochemistry: SALL4 Antibody (A7A7-R) [NBP3-32927] - Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (NBP3-32927) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32927) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: SALL4 Antibody (A7A7-R) [NBP3-32927] - Immunofluorescence analysis of paraffin-embedded human seminoma tissue labeling SALL4 with Mouse anti-SALL4 antibody (NBP3-32927) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32927, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32927_mouse-sall4-mab-a7a7-r-298202414262676.jpg)
![Immunocytochemistry/ Immunofluorescence: SALL4 Antibody (A7A7-R) [NBP3-32927] - Immunocytochemistry analysis of NCCIT cells labeling SALL4 with Mouse anti-SALL4 antibody (NBP3-32927) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-SALL4 antibody (NBP3-32927) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32927_mouse-sall4-mab-a7a7-r-29202410234247.jpg)
![Flow Cytometry: SALL4 Antibody (A7A7-R) [NBP3-32927] - Flow cytometric analysis of NCCIT cells labeling SALL4.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32927, 1ug/ml) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32927_mouse-sall4-mab-a7a7-r-29202416372112.jpg)
![Western blot analysis of NCCIT cell lysates with NBP3-32927 at 1/2,000 dilution. Predicted band size: 112 kDa, 20 µg/Lane Observed band size: 140/110/75 kDa, 20 µg/Lane Exposure time: 1 minute 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32927 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32927_-western-blot-639204590078746512.jpg)
![Western Blot: SALL4 Antibody [NB100-60559] - Western blot analysis of SALL4 using NB100-60559 at 0.1ug/ml in rat testis lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/SALL4-Antibody-Western-Blot-NB100-60559-img0006.jpg "Western Blot: SALL4 Antibody [NB100-60559] - Western blot analysis of SALL4 using NB100-60559 at 0.1ug/ml in rat testis lysate (35ug protein in RIPA buffer).")
![Western Blot: SALL4 Antibody [NB100-60559] - (0.1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SALL4-Antibody-Western-Blot-NB100-60559-img0004.jpg)
![Western Blot: Sall4 Overexpression Lysate (Adult Normal) [NBP2-06650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sall4.](http://images.novusbio.com/fullsize/Sall4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06650-img0001.jpg "Western Blot: Sall4 Overexpression Lysate (Adult Normal) [NBP2-06650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sall4.")
![Recombinant Human SALL4 Protein [H00057167-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00057167-Q01-1.jpg "Recombinant Human SALL4 Protein [H00057167-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
