S100B Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Goat Anti-Human S100B ...read more
S100B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) at 1.7 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

S100B Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271
Specificity
Detects human S100B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A4, and rhS100A6 is observed.
Source
N/A
Assay Type
abc
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1820 in the following applications:

Publications
Read Publications using
AF1820 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100B Antibody [Unconjugated]

  • beta (neural)
  • NEF
  • S100 beta
  • S100 calcium binding protein B
  • S100 calcium-binding protein B
  • S100 calcium-binding protein, beta (neural)
  • S-100 calcium-binding protein, beta chain, 10protein S100-B
  • S-100 protein beta chain
  • S-100 protein subunit beta
  • S100
  • S100B
  • S100beta

Background

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100B Antibody (AF1820)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, IHC.


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ELISA Development
(1)
IHC
(1)
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Human
(1)
Mouse
(1)
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Review for S100B Antibody (AF1820) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1820:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin S100B AF1820
Enlarge
5
reviewed by:
Lisa Ikariyama
IHC-P Mouse 08/18/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse skin tissue
SpeciesMouse
LotJTR021706B

Comments

Comments1.Tissue: formalin fixed paraffin embedded mouse skin tissue
2.Antigen retrieval: 0.01 M Citric Acid, 0.05% Tween-20
3.First antibody: Goat anti s100b (AF1820), 1:100 dilution overnight at 4 °C
4.Secondary antibody- Bio- Dondey anti Goat, 1:200 room temperature 1 hours
5.Kits used : Vectorstain ABC kit (cat: PK-4000) and DAB staining kit (Brown, cat: SK4100)
6.Counterstained with hematoxylin (blue), only nerve and hair follicle are positive.

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FAQs for S100B Antibody (AF1820) (0)

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Secondary Antibodies

 

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Blogs on S100B.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Lisa Ikariyama
08/18/2017
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol S100B
Uniprot