S100A9 Antibody [Unconjugated]

Images

 
Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat ...read more
S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. ...read more
S100A9 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 0.3 µg/mL for 1 hour at room temperature ...read more
S100A9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 5 µg/mL for 3 hours at room temperature. Cells were ...read more
Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 20 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

S100A9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse S100A9
Ala2-Lys113
Accession # P31725
Specificity
Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant mouse S100A10 and recombinant human S100B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100A9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2065 in the following applications:

Publications
Read Publications using
AF2065 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A9 Antibody [Unconjugated]

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9, also known as calgranulin B, migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A9 Antibody (AF2065)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: Confocal Microscopy, ELISA Development, Functional Assay, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Confocal Microscopy
(1)
ELISA Development
(1)
Functional Assay
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(1)
Mouse
(18)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF2065 Applications Species
N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021;36(11):109693. 2021 [PMID: 34525359] (IHC, Mouse) IHC Mouse
A Dufour, CL Bellac, U Eckhard, N Solis, T Klein, R Kappelhoff, N Fortelny, P Jobin, J Rozmus, J Mark, P Pavlidis, V Dive, SJ Barbour, CM Overall C-terminal truncation of IFN-? inhibits proinflammatory macrophage responses and is deficient in autoimmune disease Nat Commun, 2018;9(1):2416. 2018 [PMID: 29925830] (Western Blot, Human) Western Blot Human
E Källberg, S Tahvili, F Ivars, T Leanderson Induction of S100A9 homodimer formation in�vivo Biochem. Biophys. Res. Commun., 2018;0(0):. 2018 [PMID: 29679568] (Western Blot, Mouse) Western Blot Mouse
Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016;0(0):. 2016 [PMID: 27382022] (Western Blot, Mouse) Western Blot Mouse
Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons PLoS Pathog, 2016;12(5):e1005572. 2016 [PMID: 27149619] (Western Blot, Mouse) Western Blot Mouse
Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod-induced psoriasis-like inflammation Eur J Immunol, 2016;0(0):. 2016 [PMID: 27122058] (IHC-Fr, Mouse) IHC-Fr Mouse
Armstrong P, Kirkby N, Chan M, Finsterbusch M, Hogg N, Nourshargh S, Warner T Novel whole blood assay for phenotyping platelet reactivity in mice identifies ICAM-1 as a mediator of platelet-monocyte interaction. Blood, 2015;126(10):e11-8. 2015 [PMID: 26215112] (Confocal Microscopy, Mouse) Confocal Microscopy Mouse
Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E Loss of JUNB/AP-1 promotes invasive prostate cancer. Cell Death Differ, 2015;22(4):574-82. 2015 [PMID: 25526087] (Western Blot, Mouse) Western Blot Mouse
Jiao , Jingjing, Dragomir , Ana-Cris, Kocabayoglu , Peri, Rahman , Adeeb H, Chow , Andrew, Hashimoto , Daigo, Leboeuf , Marylene, Kraus , Thomas, Moran , Thomas, Carrasco-Avino , Gonzalo, Friedman , Scott L, Merad , Miriam, Aloman , Costica Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils. J Immunol, 2014;192(7):3374-82. 2014 [PMID: 24591364] (IHC, Mouse) IHC Mouse
Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model. PLoS ONE, 2014;9(2):e88924. 2014 [PMID: 24586443] (IHC, Mouse) IHC Mouse
Show All 20 Publications.

Reviews for S100A9 Antibody (AF2065) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2065:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/06/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 21228116
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23481567
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23481567

Details

Detection NotesSee PMID 23481567

Comments

CommentsPublished See PMID 23481567

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for S100A9 Antibody (AF2065). (Showing 1 - 2 of 2 FAQs).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Verified Customer
01/06/2015
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/06/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol S100A9
Uniprot