S100A8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen ...read more
S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

S100A8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse S100A8
Pro2-Glu89
Accession # P27005
Specificity
Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
S100A8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 3.5
using
AF3059 in the following applications:

Publications
Read Publications using
AF3059 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A8 Antibody [Unconjugated]

  • 60B8AG
  • CAGACP-10
  • Calgranulin A
  • calgranulin-A
  • Calprotectin L1L subunit
  • CFAG
  • CFAGL1Ag
  • CGLA
  • Cystic fibrosis antigen
  • Leukocyte L1 complex light chain
  • MA387
  • MIF
  • Migration inhibitory factor-related protein 8
  • MRP8
  • MRP-8
  • MRP8S100 calcium binding protein A8 (calgranulin A)
  • NIF
  • P8
  • protein S100-A8
  • S100 calcium binding protein A8
  • S100 calcium-binding protein A8 (calgranulin A)
  • S100 calcium-binding protein A8
  • S100A8
  • Urinary stone protein band A

Background

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A8 Antibody (AF3059)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Development, IHC, In vivo, WB.


Filter By Application
ELISA Development
(1)
IHC
(3)
In vivo
(1)
WB
(7)
All Applications
Filter By Species
Mouse
(12)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF3059 Applications Species
Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content Sci Rep, 2016;6(0):37871. 2016 [PMID: 27917875]
Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016;0(0):. 2016 [PMID: 27382022] (WB,, Mouse) WB Mouse
Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E Loss of JUNB/AP-1 promotes invasive prostate cancer. Cell Death Differ, 2015;22(4):574-82. 2015 [PMID: 25526087] (WB,, Mouse) WB Mouse
Yajima-Himuro, Sara, Oshima, Masamits, Yamamoto, Gou, Ogawa, Miho, Furuya, Madoka, Tanaka, Junichi, Nishii, Kousuke, Mishima, Kenji, Tachikawa, Tetsuhik, Tsuji, Takashi, Yamamoto, Matsuo The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. Sci Rep, 2014;4(0):4867. 2014 [PMID: 24785116] (IHC,, Mouse) IHC Mouse
Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways. Am J Pathol, 2014;184(6):1877-89. 2014 [PMID: 24726646] (IHC,, Mouse) IHC Mouse
Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Am J Pathol, 2014;184(5):1577-92. 2014 [PMID: 24631179] (IHC,, Mouse) IHC Mouse
Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model. PLoS ONE, 2014;9(2):e88924. 2014 [PMID: 24586443] (WB,, Mouse) WB Mouse
Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 2014;40(2):262-73. 2014 [PMID: 24508234] (WB,, Mouse) WB Mouse
Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis., 2014;0(0):. 2014 [PMID: 24478092] (In vivo,, Mouse) In vivo Mouse
Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation. Immunity, 2013;38(3):541-54. 2013 [PMID: 23477736] (WB,, Mouse) WB Mouse
Show All 13 Publications.

Reviews for S100A8 Antibody (AF3059) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF3059:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20098622
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20098622

Details

Detection NotesSee PMID 20098622

Comments

CommentsSee PMID 20098622Also works in Immunohistochemistry paraffin
 
reviewed by:
Anonymous
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23548910
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23548910

Details

Detection NotesSee PMID 23548910

Comments

CommentsPublished: See PMID 23548910Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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WB Video Protocol

FAQs for S100A8 Antibody (AF3059). (Showing 1 - 2 of 2 FAQs).

  1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
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Anonymous
01/07/2015
Application: WB
Species: Mouse

 
Anonymous
01/07/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol S100A8
Entrez
Uniprot