Melan-A/MART-1 Antibody (A19-P)

Images

 
Immunocytochemistry/ Immunofluorescence: MART-1 / Melan-A Antibody (A19-P) [NBP1-30151] - Staining of human hair follicle culture using NBP1-30151 at a dilution of 1:200. Image provided by Dr. H. Locher of Leiden ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.
Immunocytochemistry/ Immunofluorescence: MART-1 / Melan-A Antibody (A19-P) [NBP1-30151] - Staining of human adult facial skin using NBP1-30151 at a dilution of 1:200. Image provided by Dr. H. Locher of Leiden University ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - MART-1 / Melan-A Antibody (A19-P) [NBP1-30151] - human melanoma stained with anti-Melan A antibody shows positive cytoplasmic immunostaining ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
A19-P
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Melan-A/MART-1 Antibody (A19-P) Summary

Immunogen
Peptide derived from C-terminal sequence of human Melan A.
Specificity
This antibody is specific for the C terminal portions of Melan-A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MLANA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
For 0.5ml and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted. ICC/IF reactivity reported in scientific literature (PMID: 23817083).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP1-30151 in the following applications:

Publications
Read Publications using
NBP1-30151 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26547111).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The 7mL and 15mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for Melan-A/MART-1 Antibody (A19-P)

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melan-A/MART-1 Antibody (NBP1-30151)(4)

Reviews for Melan-A/MART-1 Antibody (NBP1-30151) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 application: ICC, IHC-P.

Reviews using NBP1-30151:
Filter by Applications
ICC
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Melan-A/MART-1 NBP1-30151
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reviewed by:
Anonymous
ICC Mouse 09/02/2013
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Summary

ApplicationImmunocytochemistry
Sample TestedMouse melanocyte cell line
SpeciesMouse
CommentsThis is a new species reactivity for this antibody.

Blocking

Blocking Details5% NGS for 30 minutes

Primary Anitbody

Dilution Ratio1:200 in PBS-T+1%BSA, overnight at 4degrees Celcius

Secondary Antibody

Secondary DescriptionGoat anti-rabbit 555
Secondary Manufacturer Cat#Alexa Fluor
Secondary Concentration1:500

Details

Detection Notesepifluorescence. Other panels: phase-contrast and brightfield
Fixation Details4% paraformaldehyde. 10 min permeabilization with ice-cold methanol.
Wash Description3x5min PBS-T

Comments

CommentsThis is a new species reactivity for this antibody.
Immunocytochemistry Melan-A/MART-1 NBP1-30151
Enlarge
reviewed by:
Anonymous
ICC Human 09/02/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman hair follicle culture
SpeciesHuman

Blocking

Blocking Details5% NGS for 30 minutes

Primary Anitbody

Dilution Ratio1:200 in PBS-T

Secondary Antibody

Secondary Descriptiongoat anti-rabbit
Secondary Manufacturer Cat#Alxa Fluor
Secondary Concentration1:500

Details

Detection Notesepifluorescence. neg contol: omission of primary antibody
Fixation Details4% paraformaldehyde. 10 min permeabilization with ice-cold methanol.
Wash Description3x5min PBS-T
Immunohistochemistry-Paraffin Melan-A/MART-1 NBP1-30151
Enlarge
reviewed by:
Anonymous
IHC-P Human 09/02/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman adult facial skin
SpeciesHuman

Blocking

Blocking Details1% BSA in PBS-0.05%Tween20, 30 min

Primary Anitbody

Dilution Ratio1:200 in PBS-T+1%BSA, overnight at RT

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit 488
Secondary Manufacturer Cat#Alexa Fluor
Secondary Concentration1:500

Details

Detection NotesConfocal, Leica SP5. Neg control: omission of primary antibody.
Fixation Details4% paraformaldehyde. Paraffin. Antigen retrieval with citrate buffer (pH 6.0), 12min at 97celcius
Wash Description3x5min PBS-T

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP1-30151) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Melan-A/MART-1 Antibody (NBP1-30151)

Discover related pathways, diseases and genes to Melan-A/MART-1 Antibody (NBP1-30151). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
09/02/2013
Application: ICC
Species: Mouse

Anonymous
09/02/2013
Application: ICC
Species: Human

Anonymous
09/02/2013
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MLANA
Entrez
Uniprot