Melan-A/MART-1 Antibody (A19-P)

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.

• Reactivity: Hu, Mu, Ca
• Applications: ICC/IF, IHC
• Clone: A19-P
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Melan-A/MART-1 Antibody (A19-P) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Peptide derived from C-terminal sequence of human Melan-A/MART-1. Antibody recognizes the epitope between Glu105 - Pro115.
• Epitope: Glu105 - Pro115
• Specificity: This antibody is specific for the C terminal portions of Melan-A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MLANA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26547111). Use in Canine reported in secitific publication PMID: 32423311

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Melan-A/MART-1 Antibody (A19-P)

• Antigen LB39-AA
• Antigen SK29-AA
• Mart 1 Melan A
• MART-1
• MART1MART-1
• MelanA
• Melan-A
• melanoma antigen recognized by T-cells 1
• MLANA
• Protein Melan-A

Background

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melan-A/MART-1 Antibody (NBP1-30151)(20)

Publications using NBP1-30151

• Kruse B, Buzzai AC, Shridhar N et al. CD4+ T cell-induced inflammatory cell death controls immune-evasive tumours Nature 2023-06-01 [PMID: 37316667] (IHC-P, Human)
  
  Details:
  IHC-P: 1:500
• Dietsch, B;Weller, C;Sticht, C;de la Torre, C;Kramer, M;Goerdt, S;G�raud, C;Wohlfeil, SA; Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern BMC cancer 2023-05-13 [PMID: 37179302] (ICC/IF, Mouse)
• Estrada J, Zhan J, Mitchell P et al. OncoVEXmGM-CSFexpands tumor antigen-specific CD8+ T-cell response in preclinical models Journal for immunotherapy of cancer 2023-05-01 [PMID: 37164449] (IHC-P, Mouse)
• Hosoya M, Kitama T, Iwabu K et al. Development of the stria vascularis in the common marmoset, a primate model Scientific reports 2022-11-17 [PMID: 36396805] (IHC-Fr, Monkey)
  
  Details:
  Dilution used in IHC-Fr 1:500
• Lee JH, Ahn Y, Lee HE et al. Wearable Surface-Lighting Micro-Light-Emitting Diode Patch for Melanogenesis Inhibition Advanced healthcare materials 2022-10-03 [PMID: 36189834] (IF/IHC)
• Eshiba S, Namiki T, Mohri Y Et al. Stem cell spreading dynamics intrinsically differentiate acral melanomas from nevi Cell reports 2021-08-03 [PMID: 34348144]
• van Beelen ESA, van der Valk WH, de Groot JCMJ et al. Migration and fate of vestibular melanocytes during the development of the human inner ear Dev Neurobiol 2020-10-19 [PMID: 33075185] (IF/IHC, Human)
• Nakano S, Abe Y, Nakajima K et al. Establishment of a mouse model for post-inflammatory hyperpigmentation Pigment Cell Melanoma Res 2020-07-04 [PMID: 32623834] (IF/IHC)
• Rahman H, Kumar D, Liu T et al. Aspirin protects melanocytes and keratinocytes against UVB-induced DNA damage in vivo J. Invest. Dermatol. 2020-06-19 [PMID: 32569596]
• Touil Y, Segaoula Z, Thuru X et al. Aggressiveness potential of spontaneous canine mucosal melanoma can dictate distinct cancer stem cell compartment behaviors in regard to their initial size and expansion abilities Stem Cells Dev. 2020-05-18 [PMID: 32423311] (IF/IHC, Canine)
• Boukhedouni N, Martins C, Darrigade AS et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo JCI Insight 2020-06-04 [PMID: 32369451] (IHC-P, Human)
• Pai CS, Huang JT, Lu X et al. Clonal Deletion of Tumor-Specific T Cells by Interferon-g Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade Immunity 2019-02-19 [PMID: 30737146] (IF/IHC, Mouse)
• Kimeswenger S, Sterniczky B, kramer A et al. Impact of infrared radiation on UVB-induced skin tumourigenesis in wild type C57BL/6 mice. Photochem. Photobiol. Sci. 2018-10-24 [PMID: 30357250] (Mouse)
• De Luca DA, Sterniczky B, Kimeswenger S et al. Ultraviolet radiation induces Melan-A-expressing cells in interfollicular epidermis in wild-type mice Arch. Dermatol. Res. 2018-05-17 [PMID: 29774387] (IHC-P, Mouse)
• Cassidy PB, Liu T, Florell SR et al. A phase II randomized placebo-controlled trial of oral N-acetylcysteine for protection of melanocytic nevi against UV-induced oxidative stress in vivo Cancer Prev Res (Phila). 2016-12-04 [PMID: 27920018] (IHC-P, Human)
• Goldschmidt BS, Viator JA. Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry. Methods Mol. Biol. 2015-12-13 [PMID: 26659796]
• Abe Y, Okamura K, Kawaguchi M et al. Rhododenol-induced leukoderma in a mouse model mimicking Japanese skin. J. Dermatol. Sci. 2015-10-27 [PMID: 26547111] (IHC-P, Mouse)
• Locher H, de Groot JC, van Iperen L et al. Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss. Dev Neurobiol 2015-11-01 [PMID: 25663387] (IHC-P, Human)
• Locher H, Frijns J H et al. TUBB3: neuronal marker or melanocyte mimic? Cell Transplant 2013-10-25 [PMID: 24152858] (IF/IHC, Human)
• Locher H, de Rooij KE, de Groot JC et al. Class III beta-tubulin, a novel biomarker in the human melanocyte lineage. Differentiation. 2013-04-01 [PMID: 23817083] (IHC-P, ICC/IF, Mouse, Human)

Reviews for Melan-A/MART-1 Antibody (NBP1-30151) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Edward van Beelen IHC-P Human 10/30/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human fetal inner ear
• Species: Human
• Lot: DB050-01-06B

reviewed by: H Locher ICC Mouse 09/02/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse melanocyte cell line
• Species: Mouse

reviewed by: H Locher ICC Human 09/02/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: Human hair follicle culture
• Species: Human

reviewed by: H Locher IHC-P Human 09/02/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human adult facial skin
• Species: Human

Bioinformatics

• Gene Symbol: MLANA
• Entrez:
  • 2315(Human)
• Uniprot:
  • Q16655()