Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7.Lane 1: S100A4 transfected lysate(11.7 KDa).Lane ...read more
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of monoclonal antibody to S100A4 on HeLa cell. Antibody concentration 15 ug/ml.
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 expression in NIH/3T3 ( Cat # L018V1).
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7 Analysis of S100A4 expression in Hela.
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7. Analysis of S100A4 expression in Raw 264.7.
ELISA: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Detection limit for recombinant GST tagged S100A4 is approximately 0.1ng/ml as a capture antibody.
S100A4 (AAH16300, 1 a.a. ~ 101 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCDEFFEGFPDKQPRKK
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA. IHC was reported in scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID: 24067916).
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
In 1x PBS, pH 7.4
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for S100A4 Antibody (1F12-1G7)
CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.