RIPK3/RIP3 Antibody Blocking Peptide

Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP] - Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of RIPK3/RIP3 Antibody blocking peptide [NBP1-77299PEP] with RIP3 antibody at 1 ug/mL. TMW 53 kda, observed molecular weight ~60 kda.

• Reactivity: Mu
• Applications: WB, AC
• Concentration: 0.2 mg/ml

RIPK3/RIP3 Antibody Blocking Peptide Summary

• Description: A blocking peptide containing 14 amino acids of mouse RIP3.

  Source: Synthetic

  (Accession #: AAF03133)

• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: RIPK3
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • Antibody Competition
  • Western Blot
• Application Notes: This peptide is useful as a blocking peptide for NBP1-77299. For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: N/A

Alternate Names for RIPK3/RIP3 Antibody Blocking Peptide

• EC 2.7.11.1
• Receptor Interacting Serine/Threonine Kinase 3
• Receptor-interacting protein 3
• receptor-interacting serine/threonine-protein kinase 3
• receptor-interacting serine-threonine kinase 3
• RIP3
• RIP-3
• RIPK3
• RIP-like protein kinase 3

Background

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for RIPK3/RIP3 Protein (NBP1-77299PEP). (Showing 1 - 3 of 3 FAQ).

1. What research areas can this product be used in?
   • All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
2. What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
   • This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

Bioinformatics

• Gene Symbol: RIPK3