Reg3B Antibody

Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg3B Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Reg3B
  Glu27-Gly175
  Accession # P35230
• Specificity: Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Reg3b
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3B Antibody

• PAP1
• Reg3B

Background

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. The mouse Reg3B precursor is 175 amino acids (aa) in length. It contains a 26 aa signal sequence plus a 149 aa mature region that possesses one C-type lectin domain (aa 38‑173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed RegA, G, and D, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg3B Antibody (AF5110)(13)

Publications using AF5110

• Janeckova, L;Stastna, M;Hrckulak, D;Berkova, L;Kubovciak, J;Onhajzer, J;Kriz, V;Dostalikova, S;Mullerova, T;Vecerkova, K;Tenglerova, M;Coufal, S;Kostovcikova, K;Blumberg, RS;Filipp, D;Basler, K;Valenta, T;Kolar, M;Korinek, V; The fate of secretory cells during intestinal homeostasis, regeneration, and tumor formation is regulated by Tcf4 bioRxiv : the preprint server for biology 2024-07-11 [PMID: 39026783] (Immunohistochemistry, Mouse)
• Huairong Zhang, Andrea Liliam Gomez Corredor, Julia Messina-Pacheco, Qing Li, George Zogopoulos, Nancy Kaddour et al. REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway Communications Biology 2021-06-07 [PMID: 34099862]
• FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Flow Cytometry, Mouse)
• R Shindo, T Katagiri, S Komazawa-S, M Ohmuraya, W Takeda, Y Nakagawa, N Nakagata, T Sakuma, T Yamamoto, C Nishiyama, T Nishina, S Yamazaki, H Kameda, H Nakano Regenerating islet-derived protein (Reg)3&beta plays a crucial role in attenuation of ileitis and colitis in mice Biochem Biophys Rep, 2020-02-07;21(0):100738. 2020-02-07 [PMID: 32072024] (IHC, Mouse)
• JA Neil, Y Matsuzawa-, E Kernbauer-, SL Schuster, S Sota, M Venzon, S Dallari, A Galvao Net, A Hine, D Hudesman, P Loke, TJ Nice, K Cadwell IFN-I and IL-22 mediate protective effects of intestinal viral infection Nat Microbiol, 2019-06-10;0(0):. 2019-06-10 [PMID: 31182797] (IHC, Mouse)
• Natalie A. Terry, Lucie V. Ngaba, Benjamin J. Wilkins, Danielle Pi, Nishi Gheewala, Klaus H. Kaestner Lipid malabsorption from altered hormonal signaling changes early gut microbial responses American Journal of Physiology-Gastrointestinal and Liver Physiology 2018-10-01 [PMID: 29953253]
• S Tremblay, G Romain, M Roux, XL Chen, K Brown, DL Gibson, S Ramanathan, A Menendez Bile acid administration elicits an intestinal antimicrobial program and reduces the bacterial burden in two mouse models of enteric infection Infect. Immun, 2017-05-23;0(0):. 5/23/2017 [PMID: 28348052]
• Hemmerling J, Heller K, Hormannsperger G, Bazanella M, Clavel T, Kollias G, Haller D Fetal exposure to maternal inflammation does not affect postnatal development of genetically-driven ileitis and colitis. PLoS ONE, 2014-05-21;9(5):e98237. 2014-05-21 [PMID: 24849654] (IHC-Fr, IHC-P, Mouse)
• Laurence Richard, Nicolas Védrenne, Jean-Michel Vallat, Benoît Funalot Characterization of Endoneurial Fibroblast-like Cells from Human and Rat Peripheral Nerves Journal of Histochemistry & Cytochemistry 2014-06-01 [PMID: 24670794]
• Zhang L, Zhang J, Shea K, Xu L, Tobin G, Knapton A, Sharron S, Rouse R Autophagy in pancreatic acinar cells in caerulein-treated mice: immunolocalization of related proteins and their potential as markers of pancreatitis. Toxicol Pathol, 2013-05-02;42(2):435-57. 5/2/2013 [PMID: 23640381]
• Egea , Laia, McAllister , Christop, Lakhdari , Omar, Minev , Ivelina, Shenouda , Steve, Kagnoff , Martin F GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa. J Immunol, 2013-01-16;190(4):1702-13. 2013-01-16 [PMID: 23325885] (IHC-P, Mouse)
• Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4. PLoS ONE, 2012-06-19;7(6):e38798. 2012-06-19 [PMID: 22723890] (IHC-P, Mouse)
• Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats. Kidney Int., 2010-10-27;79(5):518-28. 2010-10-27 [PMID: 20980976] (Western Blot, Rat)

Review for Reg3B Antibody (AF5110) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23064197
• Species: Mouse

Bioinformatics

• Gene Symbol: Reg3b
• Uniprot:
  • P35230()