Reg3B Antibody

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Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Reg3B Antibody Summary

Immunogen
E. coli-derived recombinant mouse Reg3B
Glu27-Gly175
Accession # P35230
Specificity
Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Reg3b
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF5110 in the following applications:

Publications
Read Publications using
AF5110 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3B Antibody

  • PAP1
  • Reg3B

Background

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. The mouse Reg3B precursor is 175 amino acids (aa) in length. It contains a 26 aa signal sequence plus a 149 aa mature region that possesses one C-type lectin domain (aa 38‑173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed RegA, G, and D, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Reg3B Antibody (AF5110)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
Western Blot
(1)
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(6)
Rat
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Showing Publications 1 - 7 of 7.
Publications using AF5110 Applications Species
FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 33676046] (Flow Cytometry, Mouse) Flow Cytometry Mouse
R Shindo, T Katagiri, S Komazawa-S, M Ohmuraya, W Takeda, Y Nakagawa, N Nakagata, T Sakuma, T Yamamoto, C Nishiyama, T Nishina, S Yamazaki, H Kameda, H Nakano Regenerating islet-derived protein (Reg)3&beta plays a crucial role in attenuation of ileitis and colitis in mice Biochem Biophys Rep, 2020;21(0):100738. 2020 [PMID: 32072024] (IHC, Mouse) IHC Mouse
JA Neil, Y Matsuzawa-, E Kernbauer-, SL Schuster, S Sota, M Venzon, S Dallari, A Galvao Net, A Hine, D Hudesman, P Loke, TJ Nice, K Cadwell IFN-I and IL-22 mediate protective effects of intestinal viral infection Nat Microbiol, 2019;0(0):. 2019 [PMID: 31182797] (IHC, Mouse) IHC Mouse
Hemmerling J, Heller K, Hormannsperger G, Bazanella M, Clavel T, Kollias G, Haller D Fetal exposure to maternal inflammation does not affect postnatal development of genetically-driven ileitis and colitis. PLoS ONE, 2014;9(5):e98237. 2014 [PMID: 24849654] (IHC-Fr, Mouse) IHC-Fr Mouse
Egea , Laia, McAllister , Christop, Lakhdari , Omar, Minev , Ivelina, Shenouda , Steve, Kagnoff , Martin F GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa. J Immunol, 2013;190(4):1702-13. 2013 [PMID: 23325885] (IHC-P, Mouse) IHC-P Mouse
Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4. PLoS ONE, 2012;7(6):e38798. 2012 [PMID: 22723890] (IHC-P, Mouse) IHC-P Mouse
Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats. Kidney Int., 2011;79(5):518-28. 2011 [PMID: 20980976] (Western Blot, Rat) Western Blot Rat

Review for Reg3B Antibody (AF5110) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5110:
Filter by Applications
Western Blot
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
Western Blot Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23064197
SpeciesMouse

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Anonymous -
01/08/2015
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol Reg3b
Uniprot