Donkey anti-Sheep IgG Secondary Antibody [HRP]

Images

 
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). ...read more
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was ...read more
Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP

Donkey anti-Sheep IgG Secondary Antibody [HRP] Summary

Immunogen
Sheep IgG
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Simple Western
  • Western Blot
Reviewed Applications
Read 2 Reviews rated 5
using
HAF016 in the following applications:

Publications
Read Publications using
HAF016 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [HRP]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF016)(42)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Cercopithecus aethiops, Gerbil, Ovine, Primate - Chlorocebus sabaeus (Green Monkey).

We have publications tested in 6 applications: IHC, IHC-Fr, IHC-P, Immunoprecipitation, Secondary Antibody, Western Blot.


Filter By Application
IHC
(2)
IHC-Fr
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Secondary Antibody
(1)
Western Blot
(35)
All Applications
Filter By Species
Human
(22)
Mouse
(15)
Rat
(1)
Bacteria - E. Coli
(1)
Cercopithecus aethiops
(1)
Gerbil
(1)
Ovine
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using HAF016 Applications Species
X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome Cell Reports, 2023-02-16;42(2):112133. 2023-02-16 [PMID: 36800288] (Western Blot, Mouse) Western Blot Mouse
E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity Nature Communications, 2022-11-25;13(1):7269. 2022-11-25 [PMID: 36433953] (Western Blot, Human, Mouse) Western Blot Human, Mouse
G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361539] (Western Blot, Human) Western Blot Human
S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36345944] (Western Blot, Human) Western Blot Human
N Pattaracho, N Sornkaew, W Warayanon, P Rangsinth, C Sillapacha, W Vongthip, S Chuchawank, A Prasansukl, T Tencomnao Aquilaria crassna Leaf Extract Ameliorates Glucose-Induced Neurotoxicity In Vitro and Improves Lifespan in Caenorhabditis elegans Nutrients, 2022-09-05;14(17):. 2022-09-05 [PMID: 36079924] (Western Blot, Human) Western Blot Human
W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion Nature Immunology, 2022-07-06;23(7):1021-1030. 2022-07-06 [PMID: 35794369] (Western Blot, Mouse) Western Blot Mouse
L Zhou, W Zhou, AM Joseph, C Chu, GG Putzel, B Fang, F Teng, M Lyu, H Yano, KI Andreasson, E Mekada, G Eberl, GF Sonnenberg Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation Nature Immunology, 2022-01-31;23(2):251-261. 2022-01-31 [PMID: 35102343] (Western Blot, Mouse) Western Blot Mouse
SM Præstholm, CM Correia, VE Goitea, MS Siersbæk, M Jørgensen, JF Havelund, TÅ Pedersen, NJ Færgeman, L Grøntved Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage Cell Reports, 2021-11-02;37(5):109938. 2021-11-02 [PMID: 34731602] (Western Blot, Mouse) Western Blot Mouse
PR Elliott, D Leske, J Wagstaff, L Schlicher, G Berridge, S Maslen, F Timmermann, B Ma, R Fischer, SMV Freund, D Komander, M Gyrd-Hanse Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains Cell Reports, 2021-10-05;37(1):109777. 2021-10-05 [PMID: 34610306] (Western Blot, Human) Western Blot Human
MS Szymczak, WK Reeves, MM Miller Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays Viruses, 2021-08-30;13(9):. 2021-08-30 [PMID: 34578299] (Western Blot, Cercopithecus aethiops) Western Blot Cercopithecus aethiops
Show All 42 Publications.

Reviews for IgG Antibody (HAF016) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using HAF016:
Filter by Applications
ELISA
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
ELISA IgG HAF016
Enlarge
5
reviewed by:
Verified Customer
ELISA Human 08/12/2023
View

Summary

ApplicationELISA
Sample TestedHuman serum
SpeciesHuman
LotXDP1522051

Comments

CommentsELISA for human serum
Western Blot IgG HAF016
Enlarge
5
reviewed by:
Raminder Singh
WB Human 08/16/2021
View

Summary

ApplicationWestern Blot
Sample TestedC2BBe1 cell lysate
SpeciesHuman
LotXDP1319111

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF016) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
08/12/2023
Application: ELISA
Species: Human

Raminder Singh
08/16/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG1