Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This ...read more
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Sheep IgG
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Reviewed Applications
Read 1 Review rated 5
using
HAF016 in the following applications:

Publications
Read Publications using
HAF016 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF016)(36)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Cercopithecus aethiops, Gerbil, Ovine, Primate - Chlorocebus sabaeus (Green Monkey).

We have publications tested in 5 applications: IHC, IHC-Fr, Immunoprecipitation, Secondary Antibody, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Secondary Antibody
(1)
Western Blot
(29)
All Applications
Filter By Species
Human
(18)
Mouse
(11)
Rat
(1)
Bacteria - E. Coli
(1)
Cercopithecus aethiops
(1)
Gerbil
(1)
Ovine
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using HAF016 Applications Species
L Zhou, W Zhou, AM Joseph, C Chu, GG Putzel, B Fang, F Teng, M Lyu, H Yano, KI Andreasson, E Mekada, G Eberl, GF Sonnenberg Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation Nature Immunology, 2022;23(2):251-261. 2022 [PMID: 35102343] (Western Blot, Mouse) Western Blot Mouse
SM Præstholm, CM Correia, VE Goitea, MS Siersbæk, M Jørgensen, JF Havelund, TÅ Pedersen, NJ Færgeman, L Grøntved Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage Cell Reports, 2021;37(5):109938. 2021 [PMID: 34731602] (Western Blot, Mouse) Western Blot Mouse
PR Elliott, D Leske, J Wagstaff, L Schlicher, G Berridge, S Maslen, F Timmermann, B Ma, R Fischer, SMV Freund, D Komander, M Gyrd-Hanse Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains Cell Reports, 2021;37(1):109777. 2021 [PMID: 34610306] (Western Blot, Human) Western Blot Human
MS Szymczak, WK Reeves, MM Miller Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays Viruses, 2021;13(9):. 2021 [PMID: 34578299] (Western Blot, Cercopithecus aethiops) Western Blot Cercopithecus aethiops
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021;9(1):57. 2021 [PMID: 33795008] (Western Blot, Mouse) Western Blot Mouse
T Hoefer, A Rana, B Niego, S Jagdale, HJ Albers, EE Gardiner, RK Andrews, AD Van der Me, CE Hagemeyer, E Westein Targeting shear gradient activated von Willebrand factor by the novel single-chain antibody A1 reduces occlusive thrombus formation in vitro Haematologica, 2020;0(0):. 2020 [PMID: 33054112] (Western Blot, Human) Western Blot Human
X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020;5(19):. 2020 [PMID: 33004686] (IHC, Mouse) IHC Mouse
A Celebi, C Orhan, B Seyhan, N Buyru Silencing of Wwox Increases Nuclear Import of Dvl proteins in Head and Neck Cancer J Cancer, 2020;11(14):4030-4036. 2020 [PMID: 32368285] (Western Blot, Human) Western Blot Human
W Luo, H Zhang, X Liang, R Xia, H Deng, Q Yi, L Lv, L Qian DNA methylation?regulated miR?155?5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10 Oncol. Rep., 2020;43(5):1692-1704. 2020 [PMID: 32323857] (Western Blot, Human) Western Blot Human
C Peris-Torr, MDC Plaza-Calo, R López-Domí, S Domínguez-, A Barrientos, P Carmona-Sá, JC Rodríguez- Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells Cancers (Basel), 2020;12(4):. 2020 [PMID: 32230715] (Western Blot, Human) Western Blot Human
Show All 36 Publications.

Review for IgG Antibody (HAF016) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using HAF016:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IgG HAF016
Enlarge
5
reviewed by:
Verified Customer
WB Human 08/16/2021
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Summary

ApplicationWestern Blot
Sample TestedC2BBe1 cell lysate
SpeciesHuman
LotXDP1319111

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FAQs for IgG Antibody (HAF016) (0)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
08/16/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGHG1