Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This ...read more
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Sheep IgG
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1

Applications/Dilutions

Publications
Read Publications using
HAF016 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Sodium Phosphate and Proclin 300.
Preservative
Proclin 300

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF016)(20)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bacteria - E. Coli, Ovine, Primate - Chlorocebus sabaeus (Green Monkey).

We have publications tested in 3 applications: IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(15)
All Applications
Filter By Species
Human
(11)
Mouse
(4)
Rat
(1)
Bacteria - E. Coli
(1)
Ovine
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using HAF016 Applications Species
TP Sandberg, MPME Stuart, J Oosting, RAEM Tollenaar, CFM Sier, WE Mesker Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer BMC Cancer, 2019;19(1):284. 2019 [PMID: 30922247] (Western Blot, Human) Western Blot Human
PJ Stanczyk, M Seidel, J White, C Viero, CH George, S Zissimopou, FA Lai Association of cardiac myosin binding protein-C with the ryanodine receptor channel: putative retrograde regulation? J. Cell. Sci., 2018;0(0):. 2018 [PMID: 29930088] (Western Blot, Human) Western Blot Human
JM Van Kampen, DG Kay Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease PLoS ONE, 2017;12(8):e0182896. 2017 [PMID: 28837568] (Western Blot, Mouse) Western Blot Mouse
Y Ge, TK Paisie, JRB Newman, LM McIntyre, P Concannon UBASH3A Mediates Risk for Type 1 Diabetes through Inhibition of T-Cell Receptor-Induced NF-?B Signaling Diabetes, 2017;0(0):. 2017 [PMID: 28607106] (Western Blot, Human) Western Blot Human
S Mary, HY Small, J Siwy, W Mullen, A Giri, C Delles Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat Hypertension, 2017;69(5):910-918. 2017 [PMID: 28348009] (Western Blot, Rat) Western Blot Rat
Targeting tachykinin receptors in neuroblastoma Oncotarget, 2016;0(0):. 2016 [PMID: 27888795] (Western Blot, Human) Western Blot Human
Rnd3-induced cell rounding requires interaction with Plexin-B2 J Cell Sci, 2016;0(0):. 2016 [PMID: 27656111] (Western Blot, Primate - Chlorocebus sabaeus (Green Monkey)) Western Blot Primate - Chlorocebus sabaeus (Green Monkey)
Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function J Neurosci, 2016;36(35):9201-16. 2016 [PMID: 27581460] (IHC-Fr, Mouse) IHC-Fr Mouse
Noy P, Swain R, Khan K, Lodhia P, Bicknell R Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2). FASEB J, 0;30(6):2311-23. 0 [PMID: 26939791] (Western Blot, Human) Western Blot Human
Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. PLoS ONE, 2015;10(8):e0136563. 2015 [PMID: 26305890] (Western Blot, Human) Western Blot Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol IGHG1