CD155/PVR Antibody (300907) [Unconjugated]

Images

 
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
CD155/PVR was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB25301) at 15 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
300907
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CD155/PVR Antibody (300907) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD155/PVR
Gly27-Asn343
Accession # AAH15542
Specificity
Detects human CD155/PVR in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PVR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Control
CD155/PVR Lysate (NBP2-65506)
Reviewed Applications
Read 1 Review rated 4
using
MAB25301 in the following applications:

Publications
Read Publications using
MAB25301 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD155/PVR Antibody (300907) [Unconjugated]

  • CD155 antigen
  • CD155
  • HVED
  • NECL5
  • Necl-5
  • nectin-like 5
  • Nectin-like protein 5
  • poliovirus receptor
  • PVR
  • PVS
  • PVSFLJ25946
  • Tage4

Background

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

  1. Takai, Y. et al. (2003) Cancer Sci. 94:655.
  2. Mendelsohn, C.L. et al. (1989) Cell 56:855.
  3. Koike, H. et al. (1990) EMBO J. 9:3217.
  4. Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
  5. Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
  6. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Lange, R. et al. (2001) Virology 285:218.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD155/PVR Antibody (MAB25301)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow, IHC Not Specified.


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Flow
(4)
IHC Not Specified
(1)
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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using MAB25301 Applications Species
Gur C, Ibrahim Y, Isaacson B, Yamin R, Abed J, Gamliel M, Enk J, Bar-On Y, Stanietsky-Kaynan N, Coppenhagen-Glazer S, Shussman N, Almogy G, Cuapio A, Hofer E, Mevorach D, Tabib A, Ortenberg R, Markel G, Miklic K, Jonjic S, Brennan C, Garrett W, Bachrach G, Mandelboim O Binding of the Fap2 protein of Fusobacterium nucleatum to human inhibitory receptor TIGIT protects tumors from immune cell attack. Immunity, 2015;42(2):344-55. 2015 [PMID: 25680274] (IHC Not Specified,, Human) IHC Not Specified Human
Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. Eur J Immunol, 2013;43(8):2138-50. 2013 [PMID: 23677581] (Flow,, Human) Flow Human
Madrid, Alexis S, Ganem, Don Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 2012;86(16):8693-704. 2012 [PMID: 22674989] (Flow,, Human) Flow Human
Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012;8(1):e1002486. 2012 [PMID: 22253598] (Flow,, Human) Flow Human
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene, 2011;30(23):2622-32. 2011 [PMID: 21258414] (Flow,, Human) Flow Human

Review for CD155/PVR Antibody (MAB25301) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB25301:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 02/19/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23933496
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23933496

Details

Detection NotesSee PMID 23933496

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23933496

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Recent Reviews

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Anonymous
02/19/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol PVR
Entrez
Uniprot