Home » PAPD1 » PAPD1 Research Products » PAPD1 Antibody (1D3)

PAPD1 Antibody (1D3)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of 30 ug whole cell lysate. A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000.
Western Blot: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of 30 ug whole cell lysate. A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of Hepatoma, using mtPAP antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
1D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

PAPD1 Antibody (1D3) Summary

Immunogen
The exact sequence is proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MTPAP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Publications
Read Publication using
NBP2-43686 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for PAPD1 Antibody (1D3)

  • EC 2.7.7.19
  • FLJ10486
  • mitochondrial poly(A) polymerase
  • mtPAP
  • PAP associated domain containing 1
  • PAP
  • PAP-associated domain-containing protein 1
  • PAPD1SPAX4
  • poly(A) RNA polymerase, mitochondrial
  • Polynucleotide adenylyltransferase
  • Terminal uridylyltransferase 1
  • TUTase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Reg3A Antibody
NBP2-24763
Western Blot: Reg3A Antibody [NBP2-24763] - Human intestine lysate probed with REG3A antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Reg3A Antibody [NBP2-24763] - Human intestine stained with REG3A antibody (2.5 ug/ml), peroxidase-conjugate and DAB chromogen.
(2 Publications)
Species: Hu, Pm
Applications: WB, IHC, IHC-P
PDAP1 Antibody
NBP2-38621
Immunocytochemistry/Immunofluorescence: PDAP1 Antibody [NBP2-38621] - Staining of human cell line U-251 MG shows positivity in plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: PDAP1 Antibody [NBP2-38621] - Staining of human tonsil shows cytoplasmic positivity mainly in germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Prostatic Acid
Phosphatase/ACPP Anti ...
AF6240
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human Prostatic Acid Phosphatase/ACPP protein was serially diluted 2-fold and captured by Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
(1 Publication)
Species: Hu
Applications: WB, ELISA, IHC
PAPOLA Antibody
NBP1-87345
Immunocytochemistry/Immunofluorescence: PAPOLA Antibody [NBP1-87345] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345] - Staining of human prostate shows moderate nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MRPS30 Antibody
NBP1-87343
Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MRPS30 Antibody [NBP1-87343] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Species: Hu, Rt
Applications: WB, IHC, IHC-P
PAG3 Antibody
NBP2-24614
Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. 1.0 ug/ml.
Species: Hu, Mu, Rt, Op, Pm
Applications: WB
ASAP1 Antibody
NBP2-24643
Western Blot: ASAP1 Antibody [NBP2-24643] - Analysis of ASAP1 in mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24643 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Species: Mu, Rt
Applications: WB
TUSC2 Antibody
NBP1-86794
Western Blot: TUSC2 Antibody [NBP1-86794] - Analysis in control (vector only transfected HEK293T lysate) and TUSC2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: TUSC2 Antibody [NBP1-86794] - Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.
Species: Hu
Applications: WB, ICC/IF
methyltransferase
like 9 Antibody
NBP2-31564
Western Blot: methyltransferase like 9 Antibody [NBP2-31564] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30
Immunohistochemistry-Paraffin: methyltransferase like 9 Antibody [NBP2-31564] - Staining of human prostate shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: methyltransferase like 9 Antibody [NBP2-31564] - Staining of human endometrium shows weak to moderate cytoplasmic and membranous positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
Lipin 1 Antibody
NB110-57150
Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.
Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
PNPT1 Antibody
H00087178-B01P
Western Blot: PNPT1 Antibody [H00087178-B01P] - Analysis of PNPT1 expression in rat brain.
Immunocytochemistry/Immunofluorescence: PNPT1 Antibody [H00087178-B01P] - Analysis of purified antibody to PNPT1 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: PNPT1 Antibody [H00087178-B01P] - Analysis of PNPT1 expression in IMR-32.
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF
CPEB1 Antibody
H00064506-D01P
Western Blot: CPEB1 Antibody [H00064506-D01P] - Analysis of CPEB1 expression in mouse liver.
Western Blot: CPEB1 Antibody [H00064506-D01P] - Analysis of CPEB1 expression in Hela S3 NE.
Western Blot: CPEB1 Antibody [H00064506-D01P] - Analysis of CPEB1 expression in mouse lung.
Species: Hu, Mu
Applications: WB, ELISA
NIPP1 Antibody
NBP1-90113
Western Blot: NIPP1 Antibody [NBP1-90113] - Analysis using Anti-PPP1R8 antibody NBP1-90113 (A) shows similar pattern to independent antibody NBP1-90112 (B).
Immunocytochemistry/Immunofluorescence: NIPP1 Antibody [NBP1-90113] - Staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human cerebral cortex.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
PAPD4 Antibody
NBP1-53057
Western Blot: PAPD4 Antibody [NBP1-53057] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
Exosome component
10 Antibody
NBP1-32870
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
(2 Reviews)
(3 Publications)
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
DNA polymerase
sigma Antibody
NB100-56356
Western Blot: DNA polymerase sigma Antibody [NB100-56356] - Analysis of DNA polymerase sigma in HeLa cell lysate using NB100-56356 at A) 2 ug/ml and B) 5 ug/ml.
Species: Hu
Applications: WB
Recombinant Human
PAPOLB Protein
H00056903-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
PAPD1 Antibody
(1D3)
NBP2-43686
PAPD1 Antibody (1D3)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PAPD1 Antibody (NBP2-43686)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for PAPD1 Antibody (NBP2-43686) (0)

There are no reviews for PAPD1 Antibody (NBP2-43686). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PAPD1 Antibody (NBP2-43686) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional PAPD1 Products

Bioinformatics Tool for PAPD1 Antibody (NBP2-43686)

Discover related pathways, diseases and genes to PAPD1 Antibody (NBP2-43686). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PAPD1 Antibody (NBP2-43686)

Discover more about diseases related to PAPD1 Antibody (NBP2-43686).
 

Pathways for PAPD1 Antibody (NBP2-43686)

View related products by pathway.

Blogs on PAPD1

There are no specific blogs for PAPD1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PAPD1 Antibody (1D3) and receive a gift card or discount.


Bioinformatics

Gene Symbol MTPAP
Novus 100% Guarantee