PMEL17/SILV Antibody (HMB45)

Images

 
Western Blot: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV antibody (HMB45).
Immunohistochemistry: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a ...read more
Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV Antibody [HMB45] NBP2-34638B (blue) and a matched isotype control (orange). Both ...read more
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human melanoma stained with gp100/Melanosome Monoclonal Antibody (HMB45).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human testis stained with gp100/Melanosome Monoclonal Antibody (HMB45).
Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Flow Cytometry: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV ...read more

Product Details

Summary
Reactivity Hu, Ca(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, Func, ICC/IF
Clone
HMB45
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PMEL17/SILV Antibody (HMB45) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34638)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Extract of pigmented melanoma metastases from lymph nodes
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (6038kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This monoclonal antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Multiplex Immunoassay
  • Western Blot 2-4 ug/ml
Application Notes
Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Western blot reported in scientific literature (PMID: 30561643).
Publications
Read Publications using
NBP2-44520 in the following applications:

  • 1 publication
  • MI
    1 publication
  • WB
    2 publications

Reactivity Notes

Does not react with Canine or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (HMB45)

  • D12S53EP1
  • Gp100
  • ME20
  • ME20M
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL
  • PMEL17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • SILV
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMEL17/SILV Antibody (NBP2-44520)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, MI, WB.


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FLOW
(1)
MI
(1)
WB
(2)
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(3)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PMEL
Uniprot