Immunohistochemistry: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a ...read more
Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV Antibody [HMB45] NBP2-34638B (blue) and a matched isotype control (orange). Both ...read more
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human melanoma stained with gp100/Melanosome Monoclonal Antibody (HMB45).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human testis stained with gp100/Melanosome Monoclonal Antibody (HMB45).
Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Flow Cytometry: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV ...read more
Extract of pigmented melanoma metastases from lymph nodes
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Protein A or G purified
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Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Use in Western blot reported in scientific literature (PMID: 30561643).
Read Publications using NBP2-44520 in the following applications:
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