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PDXP Antibody

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Western Blot: PDXP Antibody [NBP2-27461] - 0.1 ug/ml staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PDXP Antibody [NBP2-27461] - 0.1 ug/ml staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PDXP Antibody [NBP2-27461] - 5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Western Blot: PDXP Antibody [NBP2-27461] - NBP2-27461 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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PDXP Antibody Summary

Immunogen
Peptide with sequence C-QHDLVPHYYVES corresponding to C-Terminus according to NP_064711.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDXP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.1-0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
Western blot: Approx 32kDa band observed in Human and Rat Heart lysates and in Mouse and Rat Brain lysates (calculated MW of 31.7kDa according to NP_064711.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Tonsil.
Publications
Read Publication using NBP2-27461.

Reactivity Notes

Expected from sequence similarity: Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDXP Antibody

  • chronophin
  • CIN
  • dJ37E16.5
  • EC 3.1.3
  • EC 3.1.3.74
  • FLJ32703
  • PLP phosphatase
  • PLP
  • PLPP
  • pyridoxal (pyridoxine, vitamin B6) phosphatase
  • pyridoxal phosphate phosphatase

Background

Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP (Jang et al., 2003 (PubMed 14522954)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDXP Antibody (NBP2-27461)(1)


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Bioinformatics

Gene Symbol PDXP
Entrez
Uniprot