CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
CEA31
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free Summary

Immunogen
Human colon carcinoma extract
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-34594 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEA Antibody (NBP2-34594) (0)

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Review for CEA Antibody (NBP2-34594) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34594:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CEACAM5/CD66e NBP2-34594
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reviewed by:
Anonymous
ICC Human 04/13/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman cell
SpeciesHuman
Lot1048-4PABX180320

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEA Antibody (NBP2-34594) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34594AF350
Alexa Fluor 405 NBP2-34594AF405
Alexa Fluor 488 NBP2-34594AF488
Alexa Fluor 532 NBP2-34594AF532
Alexa Fluor 594 NBP2-34594AF594
Alexa Fluor 647 NBP2-34594AF647
Alexa Fluor 700 NBP2-34594AF700
Alexa Fluor 750 NBP2-34594AF750
Allophycocyanin NBP2-34594APC
Allophycocyanin/Cy7 NBP2-34594APCCY7
Biotin NBP2-34594B
DyLight 350 NBP2-34594UV
DyLight 405 NBP2-34594V
DyLight 488 NBP2-34594G
DyLight 550 NBP2-34594R
DyLight 594 NBP2-34594DL594
DyLight 650 NBP2-34594C
DyLight 680 NBP2-34594FR
DyLight 755 NBP2-34594IR
HRP NBP2-34594H
Janelia Fluor 549 NBP2-34594JF549
Janelia Fluor 646 NBP2-34594JF646
PE NBP2-34594PE
PE/Atto594 NBP2-34594PEATT594
PE/Cy5.5 NBP2-34594PECY55
PE/Cy7 NBP2-34594PECY7
PerCP NBP2-34594PCP

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Recent Reviews

4
5
0
4
1
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0
2
0
1
0

Anonymous
04/13/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CEACAM5