Recombinant Human CD44 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human CD44 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind biotinylated hyaluronan in a functional ELISA with an estimated Kd <0.6 nM.
Source
Mouse myeloma cell line, NS0-derived human CD44 protein
Human CD44
(Gln21-Pro220)
Accession # P16070
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln21
Protein/Peptide Type
Recombinant Proteins
Gene
CD44
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
48.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
72-86 kDa, reducing conditions
Publications
Read Publications using
3660-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD44 Fc Chimera Protein, CF

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.

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Publications for CD44 (3660-CD)(8)

We have publications tested in 3 confirmed species: Human, Bacteria, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, Cell Culture, Enzyme Assay Substrate.


Filter By Application
Binding Assay
(1)
Bioassay
(5)
Cell Culture
(1)
Enzyme Assay Substrate
(1)
All Applications
Filter By Species
Human
(6)
Bacteria
(1)
N/A
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 3660-CD Applications Species
JY Guo, CH Chiu, MJ Wang, FA Li, JY Chen Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44 J. Biomed. Sci., 2020;27(1):2. 2020 [PMID: 31898491] (Cell Culture, Human) Cell Culture Human
LL Eggink, KF Roby, R Cote, J Kenneth Ho An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides J Immunother Cancer, 2018;6(1):28. 2018 [PMID: 29665849] (Binding Assay, Human) Binding Assay Human
M Qadri, S Almadani, GD Jay, KA Elsaid Role of CD44 in Regulating TLR2 Activation of Human Macrophages and Downstream Expression of Proinflammatory Cytokines J. Immunol., 2017;0(0):. 2017 [PMID: 29196459] (Bioassay, Human) Bioassay Human
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016;6(0):37953. 2016 [PMID: 27892504] (Bioassay, N/A) Bioassay N/A
Kellett-Clarke H, Stegmann M, Barclay A, Metcalfe C CD44 Binding to Hyaluronic Acid Is Redox Regulated by a Labile Disulfide Bond in the Hyaluronic Acid Binding Site. PLoS ONE, 0;10(9):e0138137. 0 [PMID: 26379032] (Bioassay, Human) Bioassay Human
van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1. J Immunol, 2015;195(4):1763-73. 2015 [PMID: 26170391] (Bioassay, Human) Bioassay Human
Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE, 2014;9(9):e107920. 2014 [PMID: 25251283] (Enzyme Assay Substrate, Bacteria) Enzyme Assay Substrate Bacteria
Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma. Cancer Res., 2009;69(20):8050-7. 2009 [PMID: 19826051] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CD44
Uniprot