Recombinant Human Amphiregulin Protein, CF


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Amphiregulin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is 5-15 ng/mL.
E. coli-derived human Amphiregulin protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
262-AR/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Amphiregulin Protein, CF

  • Amphiregulin
  • AR
  • AREG
  • Colorectum cell-derived growth factor
  • MGC13647
  • schwannoma-derived growth factor
  • SDGF


Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid residues transmembrane precursor. Multiple forms of native AR containing either 78 or 84 amino acid residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 amino acid residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 amino acid residue form of recombinant amphiregulin in an in vitro proliferation assay using Balb/3T3 fibroblasts.

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Publications for Amphiregulin (262-AR/CF)(31)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Developmet, Western Blot, exosome culture.

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Cell Culture
ELISA (Standard)
ELISA Developmet
Western Blot
exosome culture
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using 262-AR/CF Applications Species
B Ehst, Z Wang, J Leitenberg, D McClanahan, R De La Torr, E Sawka, AG Ortega-Loa, J Strunck, T Greiling, E Simpson, Y Liu Synergistic induction of IL-23 by TNF&amp;alpha, IL-17A, and EGF in keratinocytes Cytokine, 2020;0(0):155357. 2020 [PMID: 33153894] (Cell Culture, Human) Cell Culture Human
S Liu, Y Wang, Y Han, W Xia, L Zhang, S Xu, H Ju, X Zhang, G Ren, L Liu, W Ye, Z Zhang, J Hu EREG-driven oncogenesis of Head and Neck Squamous Cell Carcinoma exhibits higher sensitivity to Erlotinib therapy Theranostics, 2020;10(23):10589-10605. 2020 [PMID: 32929368] (Bioassay, Human) Bioassay Human
M Oka, S Shimo, N Ohno, H Imai, Y Abekura, H Koseki, H Miyata, K Shimizu, M Kushamae, I Ono, K Nozaki, A Kawashima, T Kawamata, T Aoki Dedifferentiation of smooth muscle cells in intracranial aneurysms and its potential contribution to the pathogenesis Sci Rep, 2020;10(1):8330. 2020 [PMID: 32433495] (Bioassay, Human) Bioassay Human
L Wang, L Wang, H Zhang, J Lu, Z Zhang, H Wu, Z Liang AREG mediates the epithelial?mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF?&amp;kappaB signalling pathway Oncol. Rep., 2020;0(0):. 2020 [PMID: 32323797] (Bioassay, Human) Bioassay Human
S Lefort, S Tan, S Balani, B Rafn, D Pellacani, M Hirst, PH Sorensen, CJ Eaves Initiation of human mammary cell tumorigenesis by mutant KRAS requires YAP inactivation Oncogene, 2019;0(0):. 2019 [PMID: 31772328] (Bioassay, Human) Bioassay Human
Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey Transfer of Functional Cargo in Exomeres Cell Rep, 2019;0(0):. 2019 [PMID: 30956133] (Western Blot, Human) Western Blot Human
S Raimondo, L Saieva, E Vicario, M Pucci, D Toscani, M Manno, S Raccosta, N Giuliani, R Alessandro Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis J Hematol Oncol, 2019;12(1):2. 2019 [PMID: 30621731] (exosome culture, Human) exosome culture Human
C Platen, S Dreschers, LK Reiss, J Wappler, TW Orlikowsky Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases Mediators Inflamm., 2018;2018(0):4310419. 2018 [PMID: 30524196] (Bioassay, Human) Bioassay Human
J Jia, C Li, J Yang, X Wang, R Li, S Luo, Z Li, J Liu, Z Liu, Y Zheng Yes-associated protein promotes the abnormal proliferation of psoriatic keratinocytes via an amphiregulin dependent pathway Sci Rep, 2018;8(1):14513. 2018 [PMID: 30323299] (Bioassay, Human) Bioassay Human
NL Ta, K Chakraband, S Huault, AO Hueber The tyrosine phosphorylated pro-survival form of Fas intensifies the EGF-induced signal in colorectal cancer cells through the nuclear EGFR/STAT3-mediated pathway Sci Rep, 2018;8(1):12424. 2018 [PMID: 30127519] (Bioassay, Human) Bioassay Human
Show All 31 Publications.

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Gene Symbol AREG