Rad17 Antibody - BSA Free

Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Rad17 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to a N-terminal region of human RAD17.
• Specificity: Reacts with residues 2-15 [NQVTDWVDPSFDDF] of the human RAD17 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot 1:500 -1:1000
• Application Notes: This antibody is useful for Western Blot. Flow Cytometry was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Rad17 Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad17 Antibody - BSA Free

• CCYC
• cell cycle checkpoint protein (RAD17)
• cell cycle checkpoint protein RAD17
• FLJ41520
• HRAD17
• R24L
• RAD1 (S. pombe) homolog
• RAD1 homolog
• RAD17 homolog (S. pombe)
• Rad17
• Rad17-like protein
• RAD17SP
• RAD24
• RF-C activator 1 homolog
• RF-C/activator 1 homolog

Background

Signals originating from damaged DNA activate checkpoint pathways and target the mitotic apparatus via a number a distinct mechanisms. These checkpoints link damage to DNA with the components of the cell cycle through signal transduction systems. In mammals, five genes have been identified as components of DNA damage-induced checkpoint pathways: ATM, p53, p21/WAF1/CIP1, hCHK1, and 14-3-3sigma. When overexpressed in mammalian cells, hRAD17 activates p53 and causes an accumulation of G1 phase cells, suggesting an involvement of the hRAD17 gene in this checkpoint pathway.(1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad17 Antibody (NB100-173)(2)

Publications using NB100-173

• Bao, S et al. ATR/ATM-mediated phosphorylation of human Rad17 is required for fentoxic stress responses. Nature 411:969-974. 2001-01-01 [PMID: 11418864] (FLOW, Human)
• Li, L et al. hRAD17, a structural homolog of the Schizosaccharomyces pombe RAD17 cell cycle checkpoint gene, stimulates p53 accumulation. Oncogene 18: 1689-1699. 1999-01-01 [PMID: 10208430] (FLOW, WB, Human)

Bioinformatics

• Gene Symbol: RAD17
• Entrez:
  • 5884(Human)