Rad9 Antibody (93A535) - BSA Free

Images

 
Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.
Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
93A535
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Rad9 Antibody (93A535) - BSA Free Summary

Immunogen
The antibody was raised against fusion protein of human full length Rad9.
Localization
Nuclear
Specificity
In recombinant Rad9, a ~50 kDa band is observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD9A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence: see Kotmatsu et al. Immunohistochemistry-Paraffin: see Lee et al (2007) for details.
Control
Rad9 Overexpression Lysate
Publications
Read Publications using
NB120-13600 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Rad9 Antibody (93A535) - BSA Free

  • cell cycle checkpoint control protein RAD9A
  • DNA repair exonuclease rad9 homolog A
  • EC 3.1.11.2
  • hRAD9
  • RAD9 (S. pombe) homolog
  • RAD9 homolog A (S. pombe)
  • RAD9

Background

Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24457
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB
H00005810-M01J
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, ELISA(Cap), S-ELISA, WB
NBP1-33589
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-27500
Species: Mu
Applications: KO, PEP-ELISA, WB
NB100-308
Species: Hu, Mu
Applications: Func, ICC/IF, IP, In vitro, KO, WB
NBP2-01020
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-464
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NB100-104
Species: Ca, Hu, Ma, Mu, Rt
Applications: ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, WB
NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NBP2-03417
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NB100-56585
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-304
Species: Bt, Bv, Ca, Fi, Gt, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO, WB
NBP2-58116
Species: Hu
Applications: ICC/IF, KD, WB
NB100-148
Species: Ce, Ch, Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, PLA, WB
NB100-217
Species: Hu, Mu
Applications: IP, WB
NBP1-80695
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-598
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IP, WB
AF2535
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Supplier Logo

Publications for Rad9 Antibody (NB120-13600)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB120-13600 Applications Species
De Haro LP, Wray J, Williamson EA et al. Metnase promotes restart and repair of stalled and collapsed replication forks. Nucleic Acids Res. 2010 Sep [PMID: 20457750] (WB)

Details:
HEk293T cells overexpressing Metnase:1. IP/WB, Fig 6B 2. IP, Fig 6C. The RAD9 IMG-164 mAb (clone 93A535) co-IP'd V5-tagged Metnase. Metnase was detected by WB with a V5 antibody.
WB
Bai H, Madabushi A, Guan X, Lu AL. Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1. DNA Repair (Amst). 2010 May 4 [PMID: 20188637] (WB)

Details:
1. IF ( HeLaS3, HCT15, LoVo cell lines), Figs 5-7. In MNNG (DNA methylating agent) treated cells RAD9 formed discrete nuclear foci. This is in comparison to unteated cells where RAD9 appeared granulated in faint spots throughout the nucleus. In Fig 7, HeL
WB
Kedar PS, Stefanick DF, Horton JK, Wilson SH. Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition. DNA Repair (Amst). 2008 Nov 1 [PMID: 18691676] (WB)

Details:
WB (M16tsA mouse fibroblasts), Fig 2C. An ATR antibody was used for WB, and the RAD9 IMG-164 antibody was used for WB. The results showed the RAD9 co-IP'd with ATR.
WB
Guan X, Madabushi A, Chang DY et al. The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates DNA repair enzyme TDG glycosylase. Nucleic Acids Res. 2007 [PMID: 17855402]
Lee HS, Cho SB, Lee HE et al. Protein expression profiling and molecular classification of gastric cancer by the tissue array method. Clin Cancer Res. 2007 Jul 15 [PMID: 17634543]

Details:
Products cited: 1. Gastric Carcinoma Tissue Microarray (IMH-316): IHC (paraffin), Tables 1,2; Fig.1. 2. Stomach Tissue Microarray (IMH-341), matching normal tissue of IMH-316: IHC (paraffin), Tables 1,2; Fig. 1 3. Caspase-3 (IMG-144A): IHC (paraffin) on g
Shi G, Chang DY, Cheng CC et al. Physical and functional interactions between MutY glycosylase homologue (MYH) and checkpoint proteins Rad9-Rad1-Hus1. Biochem J. 2006 Nov 15 [PMID: 16879101]

Details:
IF (HeLa), Fig 9. In H202 treated HeLa cells Rad9 formed discrete nuclear foci. This is in comparison to untreated HeLa cells where Rad9 appeared granulated in faint spots throughout the nucleus.
Lee HS, Chang MS, Yang HK et al. Epstein-barr virus-positive gastric carcinoma has a distinct protein expression profile in comparison with epstein-barr virus-negative carcinoma. Clin Cancer Res. 2004 Mar 1 [PMID: 15014022] (IHC-P)

Details:
IHC (paraffin): gastric carcinoma (Table 1, Fig 3). RAD9 was expressed in the nucleus of nonneoplastic gastric mucosa. There was a loss of RAD9 expression in some neoplastic gastric cases.
IHC-P
Komatsu K, Wharton W, Hang H et al. PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. Oncogene. 2000 Nov 2 [PMID: 11077446] (ICC/IF, Human)

Details:
IF (Flow2000 human fibroblasts cells), Fig 4.
ICC/IF Human

Reviews for Rad9 Antibody (NB120-13600) (0)

There are no reviews for Rad9 Antibody (NB120-13600). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad9 Antibody (NB120-13600) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Rad9 Products

Bioinformatics Tool for Rad9 Antibody (NB120-13600)

Discover related pathways, diseases and genes to Rad9 Antibody (NB120-13600). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rad9 Antibody (NB120-13600)

Discover more about diseases related to Rad9 Antibody (NB120-13600).
 

Pathways for Rad9 Antibody (NB120-13600)

View related products by pathway.

PTMs for Rad9 Antibody (NB120-13600)

Learn more about PTMs related to Rad9 Antibody (NB120-13600).
 

Research Areas for Rad9 Antibody (NB120-13600)

Find related products by research area.

Blogs on Rad9

There are no specific blogs for Rad9, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Rad9 Antibody (93A535) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol RAD9A
Entrez
Uniprot