| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86561-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1926-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3866-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1374-SP | |||
AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation." alt="Rad17 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2-3 µg Mouse Anti-Human Rad17 Monoclonal Antibody (Catalog # MAB1926) for 1 hour on ice. Rad17-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Rad17 (left panel) was detected by Western blot using 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1926-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-173SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21602-50ul | |||
![Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NBP2-24457] - Western blot analysis of phosphorylated Rad17 (Ser645) in recombinant fusion protein containing 1) a phosphorylated serine at position 645 and 2) an unphosphorylated serine at position 645 using NBP2-24457 at 0.025 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad17 [p Ser645] Antibody [NBP2-24457] - Analysis of phosphorylated Rad17 in formalin-fixed, paraffin-embedded mouse liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-Paraffin-NBP2-24457-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86561-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Rad17 Antibody [NBP1-86561] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunohistochemistry-Paraffin-NBP1-86561-img0001.jpg)
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg)
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg)
![Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NBP2-27106-img0002.jpg "Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.")
![Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Western-Blot-NBP3-13072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17 with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-2920241114833.jpg "Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Immunocytochemistry analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17 with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad17 antibody (NBP3-32792) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Flow Cytometry: Rad17 [p Ser656] Antibody (JE63-30) [NBP3-32792] - Flow cytometric analysis of HeLa cells treated with or without 100ng/mL Calyculin A for 30 minutes labeling Rad17.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32792, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32792_rabbit-rad17-p-ser656-mab-je63-30-29202416453256.jpg)
![Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NB100-173-img0001.jpg "Western Blot: Rad17 Antibody [NB100-173] - WB detection of RAD17 protein in human heart lysate using NB100-173 at a dilution of 1:500")
![Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21602_rabbit-rad17-p-ser-656-mab-sr2259-67202310620.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.")
