XPF Antibody (OTI4E11)

Western Blot: XPF Antibody (4E11) [NBP2-01020] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.

Immunocytochemistry/Immunofluorescence: XPF Antibody (4E11) [NBP2-01020] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human tonsil using anti-XPF mouse monoclonal antibody.

Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.

Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human Kidney tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human lymphoma tissue using anti-XPF mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human prostate tissue using anti-XPF mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.84 mg/ml

XPF Antibody (OTI4E11) Summary

• Immunogen: Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERCC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 104.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.84 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XPF Antibody (OTI4E11)

• DNA excision repair protein ERCC-4
• DNA repair protein complementing XP-F cells
• EC 3.1
• ERCC11
• ERCC4
• excision repair cross-complementing rodent repair deficiency, complementationgroup 4
• xeroderma pigmentosum, complementation group F
• XPF
• XPFcomplementing defective, in Chinese hamster

Background

The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERCC4
• Entrez:
  • 2072(Human)