XPF Antibody (OTI4E11) Summary
Immunogen |
Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
ERCC4 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:100
- Immunocytochemistry/Immunofluorescence 1:100
- Immunohistochemistry 1:150
- Immunohistochemistry-Paraffin 1:150
- Western Blot 1:500-2000
|
Theoretical MW |
104.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
Preservative |
0.02% Sodium Azide |
Concentration |
0.84 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for XPF Antibody (OTI4E11)
Background
The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for XPF Antibody (NBP2-01020) (0)
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Product General Protocols
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Video Protocols
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Secondary Antibodies
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Isotype Controls
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Read the
Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on XPF.
NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair... Read full blog post.
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