XPF Antibody (OTI4E11)

Images

 
Western Blot: XPF Antibody (4E11) [NBP2-01020] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: XPF Antibody (4E11) [NBP2-01020] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human tonsil using anti-XPF mouse monoclonal antibody.
Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by ...read more
Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human Kidney tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human lymphoma tissue using anti-XPF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human prostate tissue using anti-XPF mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

XPF Antibody (OTI4E11) Summary

Immunogen
Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ERCC4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
104.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.84 mg/ml
Purity
Affinity purified

Alternate Names for XPF Antibody (OTI4E11)

  • DNA excision repair protein ERCC-4
  • DNA repair protein complementing XP-F cells
  • EC 3.1
  • ERCC11
  • ERCC4
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 4
  • xeroderma pigmentosum, complementation group F
  • XPF
  • XPFcomplementing defective, in Chinese hamster

Background

The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-704
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74611
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB100-477
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-13600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-33589
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-93321
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-41208
Species: Hu
Applications: WB
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NB100-74457
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-61060
Species: Hu, Mu
Applications: WB, ChIP, IP
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NBP2-24457
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
NB100-148
Species: Hu, Mu, Rt, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, KD
NB100-1768
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87154
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB100-2064
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
NB100-308
Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP, In vitro, KO
NB100-165
Species: Hu, Dr, Ha
Applications: ICC/IF (-), WB, IP

Publications for XPF Antibody (NBP2-01020) (0)

There are no publications for XPF Antibody (NBP2-01020).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for XPF Antibody (NBP2-01020) (0)

There are no reviews for XPF Antibody (NBP2-01020). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XPF Antibody (NBP2-01020) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74883AF350
Alexa Fluor 405 NBP2-74883AF405
Alexa Fluor 488 NBP2-74883AF488
Alexa Fluor 532 NBP2-74883AF532
Alexa Fluor 594 NBP2-74883AF594
Alexa Fluor 647 NBP2-74883AF647
Alexa Fluor 700 NBP2-74883AF700
Alexa Fluor 750 NBP2-74883AF750
Allophycocyanin NBP2-74883APC
Allophycocyanin/Cy7 NBP2-74883APCCY7
Biotin NBP2-74883B
DyLight 350 NBP2-74883UV
DyLight 405 NBP2-74883V
DyLight 488 NBP2-74883G
DyLight 550 NBP2-74883R
DyLight 594 NBP2-74883DL594
DyLight 650 NBP2-74883C
DyLight 680 NBP2-74883FR
DyLight 755 NBP2-74883IR
FITC NBP2-74883F
HRP NBP2-74883H
Janelia Fluor 549 NBP2-74883JF549
Janelia Fluor 646 NBP2-74883JF646
PE NBP2-74883PE
PE/Atto594 NBP2-74883PEATT594
PE/Cy5.5 NBP2-74883PECY55
PE/Cy7 NBP2-74883PECY7
PerCP NBP2-74883PCP

Additional XPF Products

Bioinformatics Tool for XPF Antibody (NBP2-01020)

Discover related pathways, diseases and genes to XPF Antibody (NBP2-01020). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for XPF Antibody (NBP2-01020)

Discover more about diseases related to XPF Antibody (NBP2-01020).
 

Pathways for XPF Antibody (NBP2-01020)

View related products by pathway.

PTMs for XPF Antibody (NBP2-01020)

Learn more about PTMs related to XPF Antibody (NBP2-01020).
 

Research Areas for XPF Antibody (NBP2-01020)

Find related products by research area.

Blogs on XPF.

NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our XPF Antibody (OTI4E11) and receive a gift card or discount.

Bioinformatics

Gene Symbol ERCC4
Entrez