Claspin Antibody

Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Claspin Antibody [NB100-247] - Detection of human Claspin on HeLa whole cell lysate using NB100-247.

Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Claspin Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLSPN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1 - 5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Claspin Antibody

• claspin homolog (Xenopus laevis)
• claspin homolog
• Claspin
• CLSPN
• hClaspin
• MGC131612
• MGC131613
• MGC131615

Background

Claspin is a cell cycle regulated protein. It plays a critical role in replication checkpoint control and interacts with the checkpoint proteins ATR and Rad9. It also associates with Chk1, but only following replication stress or other types of DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claspin Antibody (NB100-247)(12)

Publications using NB100-247

• Speroni J, Federico MB, Mansilla SF et al. Kinase-independent function of checkpoint kinase 1 (Chk1) in the replication of damaged DNA. Proc Natl Acad Sci U S A 2012 May [PMID: 22529391]
• Guervilly JH, Renaud E, Takata M et al. USP1 deubiquitinase maintains phosphorylated CHK1 by limiting its DDB1-dependent degradation. Hum Mol Genet 2011 Jun [PMID: 21389083]
• Despras E, Daboussi F, Hyrien O et al. ATR/Chk1 pathway is essential for resumption of DNA synthesis and cell survival in UV-irradiated XP variant cells. Hum Mol Genet 2010 May [PMID: 20123862]
• Castriconi R, Daga A, Dondero A et al. NK cells recognize kill human glioblastoma cells with stem cell-like properties. J Immunol;182(6):3530-9. 2009 Mar 15. [PMID: 19265131]
• Yang XH, Shiotani B, Classon M et al. Chk1 and Claspin potentiate PCNA ubiquitination. Genes Dev 2008 May [PMID: 18451105]
• Guervilly JH, Mace-Aime G, Rosselli F et al. Loss of CHK1 function impedes DNA damage-induced FANCD2 monoubiquitination but normalizes the abnormal G2 arrest in Fanconi anemia. Hum Mol Genet 2008 Mar [PMID: 18029388]
• Buchsbaum S, Morris C, Bochard V, Jalinot P. Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle. Oncogene 2007 Aug 2 [PMID: 17310990] (Human)
• Ge Li, RT et al. Phosphatase type 2A-dependent -independent pathways for ATR phosphorylation of Chk1. J Biol Chem 282(10): 7287-7298. 2007 [PMID: 17210576]
• Wang X, Zou L, Lu T et al. Rad17 phosphorylation is required for claspin recruitment and Chk1 activation in response to replication stress. Mol Cell 2006 Aug [PMID: 16885023]
• MailN, Bekker-Jensen S, Bartek J et al. Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress. Mol Cell 2006 Aug [PMID: 16885021]
• Bennett LN, Clarke PR. Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation. FEBS Lett 2006 Jul [PMID: 16828751]
• Clarke CA, Bennett LN, Clarke PR et al. Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway. J Biol Chem 2005 Oct [PMID: 16123041]

Bioinformatics

• Gene Symbol: CLSPN
• Entrez:
  • 63967(Human)
• Uniprot:
  • Q9HAW4()