POLR1A Antibody - BSA Free

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Western Blot: POLR1A Antibody - BSA Free [NBP3-47093] - HeLa cells were subjected to SDS PAGE followed by western blot with (POLR1A antibody) at dilution of 1:1000

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

POLR1A Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit POLR1A Antibody - BSA Free (NBP3-47093) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
polymerase(RNA) I polypeptide A, 194kDa. (Uniprot# O95602)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR1A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500-1:2000
Application Notes
Theoretical Molecular Weight is 190-195 kDa

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% Glycerol, pH (7.3)
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLR1A Antibody - BSA Free

  • DNA-directed RNA polymerase I subunit RPA1
  • A190
  • POLR1A
  • polymerase (RNA) I polypeptide A, 194kDa
  • RPA1
  • RPA194
  • RPO14
  • RPO1-4

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POLR1A