RPA14 Antibody (11.1)

Images

 
Western Blot: RPA14 Antibody (11.1) [NB100-159] - A. 30 ug A549 whole cell lysate/extract B. 30 ug MCF-7 whole cell lysate/extract 15 % SDS-PAGE RPA14 antibody [11.1] dilution: 1:1000
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Paraffin-embedded Mahlarvu xenograft. RPA14 antibody [11.1] dilution: 1:200.
Western Blot: RPA14 Antibody (11.1) [NB100-159] - Sample (30 ug of whole cell lysate) A: MCF7 15% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
11.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

RPA14 Antibody (11.1) Summary

Immunogen
Full-length human RPA-14 expressed in E. coli..
Localization
Nuclear
Specificity
NB100-159 is specific for human and mouse RPA14.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 0.1-2 ug/ml
Application Notes
WB: Predicted MW is 14 kDa.
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-159 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RPA14 Antibody (11.1)

  • REPA3Replication factor A protein 3
  • replication protein A 14 kDa subunit
  • replication protein A3 (14kD)
  • replication protein A3, 14kDa
  • RF-A protein 3
  • RP-A p14
  • RPA14

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPA14 Antibody (NB100-159)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Human
(2)
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Bioinformatics

Gene Symbol RPA3
Entrez
Uniprot