RPA14 Antibody (11.1)

Images

 
Western Blot: RPA14 Antibody (11.1) [NB100-159] - A: 30 ug A549 whole cell lysate/extract. B: 30 ug MCF-7 whole cell lysate/extract. Separated by 15% SDS-PAGE. RPA14 antibody [11.1] at 1:1000.
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Human osteosarcoma section, 4% formaldehyde fixed. Incubated with the primary antibody for 10-15 hours. IHC-P image submitted by a verified customer ...read more
Western Blot: RPA14 Antibody (11.1) [NB100-159] - 30 ug of MCF7 whole cell lysate separated by 15% SDS-PAGE. Antibody at 1:500.
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Mahlarvu xenograft. RPA14 antibody [11.1] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
11.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RPA14 Antibody (11.1) Summary

Immunogen
Full-length human RPA-14 expressed in E. coli..
Localization
Nuclear
Specificity
NB100-159 is specific for human and mouse RPA14.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 0.1-2 ug/ml
  • Western Blot 0.1-2 ug/ml
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-159 in the following applications:

Publications
Read Publications using
NB100-159 in the following applications:

  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RPA14 Antibody (11.1)

  • REPA3Replication factor A protein 3
  • replication protein A 14 kDa subunit
  • replication protein A3 (14kD)
  • replication protein A3, 14kDa
  • RF-A protein 3
  • RP-A p14
  • RPA14

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2204
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-23017
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
NBP2-32016
Species: Hu
Applications: IHC, IHC-P
H00084172-M10
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NBP1-48002
Species: Ca, Hu, Pm, Pm
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF6457
Species: Hu
Applications: WB
NB100-464
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NB600-1031
Species: Hu, Mu, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00029935-B01P
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-16182
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-73857
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, IHC, WB
NB100-308
Species: Hu, Mu
Applications: Func, ICC/IF, IP, In vitro, KO, WB
NBP1-89342
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-45946
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32716
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Publications for RPA14 Antibody (NB100-159)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species

Review for RPA14 Antibody (NB100-159) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-159:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin RPA14 NB100-159
Enlarge
5
reviewed by:
Michael Nowak
Immunohistochemistry-Paraffin Human 10/10/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedOsteosarcoma
SpeciesHuman
Lot11.1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RPA14 Antibody (NB100-159) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RPA14 Products

Bioinformatics Tool for RPA14 Antibody (NB100-159)

Discover related pathways, diseases and genes to RPA14 Antibody (NB100-159). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RPA14 Antibody (NB100-159)

Discover more about diseases related to RPA14 Antibody (NB100-159).
 

Pathways for RPA14 Antibody (NB100-159)

View related products by pathway.

PTMs for RPA14 Antibody (NB100-159)

Learn more about PTMs related to RPA14 Antibody (NB100-159).
 

Research Areas for RPA14 Antibody (NB100-159)

Find related products by research area.

Blogs on RPA14

There are no specific blogs for RPA14, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Michael Nowak
10/10/2021
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol RPA3
Entrez
Uniprot