XPA Antibody

Images

 
Western Blot: XPA Antibody [NB100-93321] - Detection of Human XPA by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa, cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: XPA Antibody [NB100-93321] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-93321 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 ...read more
Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - FFPE section of human ovarian tumor. Affinity purified rabbit anti-XPA used at a dilution of 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

XPA Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human xeroderma pigmentosum, complementation group A using the numbering given in entry NP_000371.1 (GeneID 7507).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Control
XPA Lysate (NBL1-17907)
XPA Lysate (NBP2-64623)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPA Antibody

  • DNA repair protein complementing XP-A cells
  • excision repair-controlling
  • Xeroderma pigmentosum group A-complementing protein
  • xeroderma pigmentosum, complementation group A
  • XP1
  • XPA
  • XPACXP1

Background

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for XPA Antibody (NB100-93321). (Showing 1 - 1 of 1 FAQ).

  1. What's the size of protein that your XPA antibody (NB100-93321) detects?
    • Xeroderma pigmentosum group A-complementing protein (XPA) has an expected molecular weight of approximately 31 kD and this protein undergoes post-translational modifications (PTMs) such as phosphorylation as well as ubiquitination that leads to small increase in its observed molecular weight in Western blots. Our XPA antibody (NB100-93321) has been validated in whole cell lysate from HeLa cells, wherein it detected specific single band at 39kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for XPA Antibody (NB100-93321)

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Diseases for XPA Antibody (NB100-93321)

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Pathways for XPA Antibody (NB100-93321)

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PTMs for XPA Antibody (NB100-93321)

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Research Areas for XPA Antibody (NB100-93321)

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Blogs on XPA.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Bioinformatics

Gene Symbol XPA
Entrez
Uniprot