XPA Antibody

Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.

Immunoprecipitation: XPA Antibody [NB100-93321] - Detection of human XPA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPA antibody NB100-93321 (lot NB100-93321-3) used for IP at 6 ug per reaction. XPA was also immunoprecipitated by a previous lot of this antibody (lot NB100-93321-2) and another rabbit anti-XPA antibody. For blotting immunoprecipitated XPA, NB100-93321 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

XPA Antibody Summary

• Description: Novus Biologicals Rabbit XPA Antibody (NB100-93321) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human xeroderma pigmentosum, complementation group A using the numbering given in entry NP_000371.1 (GeneID 7507).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  XPA Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XPA Antibody

• DNA repair protein complementing XP-A cells
• excision repair-controlling
• Xeroderma pigmentosum group A-complementing protein
• xeroderma pigmentosum, complementation group A
• XP1
• XPA
• XPACXP1

Background

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for XPA Antibody (NB100-93321). (Showing 1 - 1 of 1 FAQ).

1. What's the size of protein that your XPA antibody (NB100-93321) detects?
   • Xeroderma pigmentosum group A-complementing protein (XPA) has an expected molecular weight of approximately 31 kD and this protein undergoes post-translational modifications (PTMs) such as phosphorylation as well as ubiquitination that leads to small increase in its observed molecular weight in Western blots. Our XPA antibody (NB100-93321) has been validated in whole cell lysate from HeLa cells, wherein it detected specific single band at 39kDa.

Bioinformatics

• Gene Symbol: XPA
• Entrez:
  • 7507(Human)
• Uniprot:
  • NP_000371.1(Human)