POLR1B Antibody (4H6) - Azide and BSA Free

Western Blot: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody (M10), clone 4H6. Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of monoclonal antibody to POLR1B on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: POLR1B Antibody (4H6) [H00084172-M10] - Detection limit for recombinant GST tagged POLR1B is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POLR1B Antibody (4H6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse POLR1B Antibody (4H6) - Azide and BSA Free (H00084172-M10) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: POLR1B (NP_061887, 963 a.a. ~ 1072 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVK
• Specificity: POLR1B (4H6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR1B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLR1B Antibody (4H6) - Azide and BSA Free

• DNA-directed RNA polymerase I 135 kDa polypeptide
• DNA-directed RNA polymerase I 135kDa polypeptide
• DNA-directed RNA polymerase I subunit RPA2
• EC 2.7.7.6
• FLJ10816
• FLJ21921
• MGC131780
• polymerase (RNA) I polypeptide B, 128kDa
• RNA polymerase I subunit 2
• RPA116
• RPA135
• RPA2
• Rpo1-2

Background

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR1B
• Entrez:
  • 84172(Human)
• Uniprot:
  • NP_061887(Human)