RPA2 [p Ser4, p Ser8] Antibody

Images

 
Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). ...read more
Immunocytochemistry/ Immunofluorescence: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and EPE treated cells (right). Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 ...read more
Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In ...read more
Biological Strategies: Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM ...read more
Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of ...read more
Independent Antibodies: Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm-OrSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RPA2 [p Ser4, p Ser8] Antibody Summary

Immunogen
Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID
Modification
p Ser4, p Ser8
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:5000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Knockdown Validated
  • Simple Western
  • Western Blot 1:2000-1:10000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Simple Western reported by an internal validation. Separated by Charge, antibody dilution of 1:50.
Publications
Read Publications using
NBP1-23017 in the following applications:

  • 3 publications
  • KD
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPA2 [p Ser4, p Ser8] Antibody

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Background

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPA2 Antibody (NBP1-23017)(37)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, KD, WB.


Filter By Application
ICC/IF
(3)
KD
(1)
WB
(7)
All Applications
Filter By Species
Human
(9)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NBP1-23017 Applications Species
de Krijger I, FOhr B, PErez SH et al. MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair Nature communications Sep 14 2021 [PMID: 34521823] (WB, Human) WB Human
Kumar S, Buon L, Talluri S et al. Integrated genomics and comprehensive validation reveal drivers of genomic evolution in esophageal adenocarcinoma Communications biology May 24 2021 [PMID: 34031527]
Li H, Ma L, Bian X et al. FK228 sensitizes radioresistant small cell lung cancer cells to radiation Clinical epigenetics Feb 25 2021 [PMID: 33632300] (Human) Human
Bian X, Wang X, Zhang Q et al. The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer Front. Oncol. Oct 8 2020 [PMID: 33134168] (WB, Human) WB Human
Bartek J, Merchut-Maya JM, Maya-Mendoza A et al. Cancer cell stemness, responses to experimental genotoxic treatments, cytomegalovirus protein expression and DNA replication stress in pediatric medulloblastomas Cell Cycle Feb 13 2020 [PMID: 32054408] (Human) Human
Wang H, Zhang S et al. Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells. Oncogene Jan 4 2020 [PMID: 32029902] (ICC/IF, Human) ICC/IF Human
Fagan-Solis KD, Simpson DA, Kumar RJ et al. A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability Cell Rep Feb 4 2020 [PMID: 32023457] (ICC/IF, Mouse) ICC/IF Mouse
Warren N, Eastman A. Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe. J. Biol. Chem. Dec 20 2018 [PMID: 30573684] (WB, Human) WB Human
Simonetta M, de Krijger I, Serrat J et al. H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2. Cell Cycle. [PMID: 29160738] (WB, Human) WB Human
Qu J, Sun W, Zhong J et al. Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells J. Cell Biol. 2017 Jul 24 [PMID: 28739677] (WB, Human) WB Human
Show All 37 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPA2 Antibody (NBP1-23017) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-23017AF350
Alexa Fluor 405 NBP1-23017AF405
Alexa Fluor 488 NBP1-23017AF488
Alexa Fluor 532 NBP1-23017AF532
Alexa Fluor 594 NBP1-23017AF594
Alexa Fluor 647 NBP1-23017AF647
Alexa Fluor 700 NBP1-23017AF700
Alexa Fluor 750 NBP1-23017AF750
DyLight 405 NBP1-23017V
DyLight 488 NBP1-23017G
DyLight 550 NBP1-23017R
DyLight 594 NBP1-23017DL594
DyLight 680 NBP1-23017FR
DyLight 755 NBP1-23017IR
Janelia Fluor 549 NBP1-23017JF549
Janelia Fluor 646 NBP1-23017JF646
PE NBP1-23017PE

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Bioinformatics

Gene Symbol RPA2
Entrez
Uniprot