SSB Antibody (OTI2C8)

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Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.
Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.
Immunoprecipitation: SSB Antibody (OTI2C8) [NBP1-48002] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T ...read more

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
OTI2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.8 mg/ml

SSB Antibody (OTI2C8) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SSB expression vector
Specificity
This antibody is specific for Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000-2000
Application Notes
IP was tested using 2ug of antibody per 500ul at 0.5mg/ml overexpression lysate. Optimal dilutions for other samples will need to be determined by the end user.
Theoretical MW
46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SSB Antibody (OTI2C8)

  • La autoantigen
  • La ribonucleoprotein
  • lupus La protein
  • member 3
  • Sjoegren syndrome type B antigen
  • Sjogren syndrome antigen B (autoantigen La)
  • SS-B
  • SS-B/La protein

Background

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaper

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SSB
Entrez